Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes

20 pages, 8 figures, 4 tables, supplementary data https://doi.org/10.1093/femsec/fiab114

Detalhes bibliográficos
Autores: Fermani, Paulina, Metz, Sebastian, Balagué, Vanessa, Descy, Jean-Pierre, Morana, Cedric, Logares, Ramiro, Massana, Ramon, Sarmento, Hugo
Formato: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/252518
Acesso em linha:http://hdl.handle.net/10261/252518
Access Level:acceso abierto
Palavra-chave:Protists
Metabarcoding
Diversity
Novelty
Great Lakes
África
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spelling Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great LakesFermani, PaulinaMetz, SebastianBalagué, VanessaDescy, Jean-PierreMorana, CedricLogares, RamiroMassana, RamonSarmento, HugoProtistsMetabarcodingDiversityNoveltyGreat LakesÁfrica20 pages, 8 figures, 4 tables, supplementary data https://doi.org/10.1093/femsec/fiab114East African Great Lakes are old and unique natural resources heavily utilized by their bordering countries. In those lakes, ecosystem functioning is dominated by pelagic processes, where microorganisms are key components; however, protistan diversity is barely known. We investigated the community composition of small eukaryotes (<10 µm) in surface waters of four African Lakes (Kivu, Edward, Albert and Victoria) by sequencing the 18S rRNA gene. Moreover, in the meromictic Lake Kivu, two stations were vertically studied. We found high protistan diversity distributed in 779 operational taxonomic units (OTUs), spanning in 11 high-rank lineages, being Alveolata (31%), Opisthokonta (20%) and Stramenopiles (17%) the most represented supergroups. Surface protistan assemblages were associated with conductivity and productivity gradients, whereas depth had a strong effect on protistan community in Kivu, with higher contribution of heterotrophic organisms. Approximately 40% of OTUs had low similarity (<90%) with reported sequences in public databases; these were mostly coming from deep anoxic waters of Kivu, suggesting a high extent of novel diversity. We also detected several taxa so far considered exclusive of marine ecosystems. Our results unveiled a complex and largely undescribed protistan community, in which several lineages have adapted to different niches after crossing the salinity boundarThis study was supported by the Spanish Ministry of Science and Technology (AGLOM project CGL2010-11556-E), the Belgian Federal Science Policy Office—BELSPO (Project EAGLES), the Fonds de la Recherche Scientifique—FNRS (Project MICKI—Microbial diversity and processes in Lake Kivu), ‘Damas’ mission, by FNRS and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP process 2014/14139-3). PF was supported by the fellow ‘Bourse de séjour scientifique’, by FNRS and Programa de Financiamiento Parcial de Estadías Breves en el Exterior para Becarios Postdoctorales by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET—Argentina). HS was supported by Conselho Nacional de Desenvolvimento e Pesquisa Tecnológica (CNPq productivity grant 309514/2017-7)With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewedFederation of European Microbiological SocietiesMinisterio de Ciencia y Tecnología (España)Belgian Science Policy OfficeFonds de la Recherche Scientifique (Fédération Wallonie-Bruxelles)Fundação de Amparo à Pesquisa do Estado de São PauloConsejo Nacional de Investigaciones Científicas y Técnicas (Argentina)Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/252518reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1093/femsec/fiab114Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2525182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes
title Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes
spellingShingle Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes
Fermani, Paulina
Protists
Metabarcoding
Diversity
Novelty
Great Lakes
África
title_short Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes
title_full Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes
title_fullStr Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes
title_full_unstemmed Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes
title_sort Microbial eukaryote assemblages and potential novel diversity in four tropical East African Great Lakes
dc.creator.none.fl_str_mv Fermani, Paulina
Metz, Sebastian
Balagué, Vanessa
Descy, Jean-Pierre
Morana, Cedric
Logares, Ramiro
Massana, Ramon
Sarmento, Hugo
author Fermani, Paulina
author_facet Fermani, Paulina
Metz, Sebastian
Balagué, Vanessa
Descy, Jean-Pierre
Morana, Cedric
Logares, Ramiro
Massana, Ramon
Sarmento, Hugo
author_role author
author2 Metz, Sebastian
Balagué, Vanessa
Descy, Jean-Pierre
Morana, Cedric
Logares, Ramiro
Massana, Ramon
Sarmento, Hugo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia y Tecnología (España)
Belgian Science Policy Office
Fonds de la Recherche Scientifique (Fédération Wallonie-Bruxelles)
Fundação de Amparo à Pesquisa do Estado de São Paulo
Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Protists
Metabarcoding
Diversity
Novelty
Great Lakes
África
topic Protists
Metabarcoding
Diversity
Novelty
Great Lakes
África
description 20 pages, 8 figures, 4 tables, supplementary data https://doi.org/10.1093/femsec/fiab114
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/252518
url http://hdl.handle.net/10261/252518
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1093/femsec/fiab114
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Federation of European Microbiological Societies
publisher.none.fl_str_mv Federation of European Microbiological Societies
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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