Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time

Prostaglandin F2α (PGF2α) has been used to improve reproductive performance in swine. The goal of the present work was to determine how the addition of PGF2α affects boar sperm quality. Eleven different treatments were evaluated: eight with only PGF2α (0.625, 1.25, 2.50, 5, 10, 12.50, 25 and 50 mg P...

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Autores: Yeste Oliveras, Marc, Briz González, Maria Dolors, Pinart Nadal, Elisabeth, Sancho Badell, Sílvia, Garcia Gil, Núria, Badia Brea, Maria Elena, Bassols Casadevall, Judit, Pruneda Sais, Anna, Bussalleu Muntada, Eva, Casas Roqueta, Isabel, Bonet, Sergi
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2008
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/25981
Acesso em linha:http://hdl.handle.net/10256/25981
Access Level:Acceso aberto
Palavra-chave:Senglar -- Espermatozoides
Wild boar -- Spermatozoa
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spelling Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling timeYeste Oliveras, MarcBriz González, Maria DolorsPinart Nadal, ElisabethSancho Badell, SílviaGarcia Gil, NúriaBadia Brea, Maria ElenaBassols Casadevall, JuditPruneda Sais, AnnaBussalleu Muntada, EvaCasas Roqueta, IsabelBonet, SergiSenglar -- EspermatozoidesWild boar -- SpermatozoaProstaglandin F2α (PGF2α) has been used to improve reproductive performance in swine. The goal of the present work was to determine how the addition of PGF2α affects boar sperm quality. Eleven different treatments were evaluated: eight with only PGF2α (0.625, 1.25, 2.50, 5, 10, 12.50, 25 and 50 mg PGF2α/100 ml) and three binary treatments (0.625 mg PGF2α/100 ml + 200 μg/ml hyaluronic acid (HA), 1.25 mg PGF2α/100 ml + 200 μg/ml HA, 0.625 mg PGF2α/100 ml + 7.5 μM caffeine (Caf)). All these substances were added to 16 ejaculates from 16 healthy and sexually mature boars (n = 16), and each ejaculate was considered as a replicate. Our study also assessed the effects of these 11 treatments over different periods of preservation. Sperm quality was tested immediately after the addition of treatments (time 0), and after 1, 3, 6 and 10 days of cooling at 15 °C. To evaluate sperm quality, five parameters were analysed: (1) sperm viability, acrosome and mitochondrial sheath integrity (using a multiple fluorochrome-staining test), (2) sperm motility, (3) sperm morphology and (4) agglutination (using a computer assisted system) and (5) osmotic resistance (using the ORT). Parametric (analysis of variance for repeated measures) and non-parametric tests (Friedman test) were used as statistical analyses. Treatments with PGF2α concentrations higher than 12.5 mg/100 ml were cytotoxic while the others did not damage boar spermatozoa. Thus, the other treatments may be used to produce profitable effects without adverse effects. Moreover, the addition of PGF2α at 5 mg/100 ml to sperm diluted in BTS may maintain sperm viability and motility better after 6 days of cooling, because significant differences were observed (P < 0.05) compared with control at the same timeElsevier2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionpeer-reviewed16 p.application/pdfhttp://hdl.handle.net/10256/25981http://hdl.handle.net/10256/25981© Animal Reproduction Science, 2008, vol. 108, núm. 1-2, p. 180-195Articles publicats (D-B)Yeste Oliveras, Marc Briz González, Maria Dolors Pinart Nadal, Elisabeth Sancho Badell, Sílvia Garcia Gil, Núria Badia Brea, Maria Elena Bassols Casadevall, Judit Pruneda Sais, Anna Bussalleu Muntada, Eva Casas Roqueta, Isabel Bonet, Sergi 2008 Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time Animal Reproduction Science 108 1-2 180 195reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2007.08.008info:eu-repo/semantics/altIdentifier/issn/0378-4320info:eu-repo/semantics/altIdentifier/eissn/1873-2232Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/259812026-05-29T05:05:01Z
dc.title.none.fl_str_mv Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
title Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
spellingShingle Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
Yeste Oliveras, Marc
Senglar -- Espermatozoides
Wild boar -- Spermatozoa
title_short Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
title_full Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
title_fullStr Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
title_full_unstemmed Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
title_sort Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time
dc.creator.none.fl_str_mv Yeste Oliveras, Marc
Briz González, Maria Dolors
Pinart Nadal, Elisabeth
Sancho Badell, Sílvia
Garcia Gil, Núria
Badia Brea, Maria Elena
Bassols Casadevall, Judit
Pruneda Sais, Anna
Bussalleu Muntada, Eva
Casas Roqueta, Isabel
Bonet, Sergi
author Yeste Oliveras, Marc
author_facet Yeste Oliveras, Marc
Briz González, Maria Dolors
Pinart Nadal, Elisabeth
Sancho Badell, Sílvia
Garcia Gil, Núria
Badia Brea, Maria Elena
Bassols Casadevall, Judit
Pruneda Sais, Anna
Bussalleu Muntada, Eva
Casas Roqueta, Isabel
Bonet, Sergi
author_role author
author2 Briz González, Maria Dolors
Pinart Nadal, Elisabeth
Sancho Badell, Sílvia
Garcia Gil, Núria
Badia Brea, Maria Elena
Bassols Casadevall, Judit
Pruneda Sais, Anna
Bussalleu Muntada, Eva
Casas Roqueta, Isabel
Bonet, Sergi
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Senglar -- Espermatozoides
Wild boar -- Spermatozoa
topic Senglar -- Espermatozoides
Wild boar -- Spermatozoa
description Prostaglandin F2α (PGF2α) has been used to improve reproductive performance in swine. The goal of the present work was to determine how the addition of PGF2α affects boar sperm quality. Eleven different treatments were evaluated: eight with only PGF2α (0.625, 1.25, 2.50, 5, 10, 12.50, 25 and 50 mg PGF2α/100 ml) and three binary treatments (0.625 mg PGF2α/100 ml + 200 μg/ml hyaluronic acid (HA), 1.25 mg PGF2α/100 ml + 200 μg/ml HA, 0.625 mg PGF2α/100 ml + 7.5 μM caffeine (Caf)). All these substances were added to 16 ejaculates from 16 healthy and sexually mature boars (n = 16), and each ejaculate was considered as a replicate. Our study also assessed the effects of these 11 treatments over different periods of preservation. Sperm quality was tested immediately after the addition of treatments (time 0), and after 1, 3, 6 and 10 days of cooling at 15 °C. To evaluate sperm quality, five parameters were analysed: (1) sperm viability, acrosome and mitochondrial sheath integrity (using a multiple fluorochrome-staining test), (2) sperm motility, (3) sperm morphology and (4) agglutination (using a computer assisted system) and (5) osmotic resistance (using the ORT). Parametric (analysis of variance for repeated measures) and non-parametric tests (Friedman test) were used as statistical analyses. Treatments with PGF2α concentrations higher than 12.5 mg/100 ml were cytotoxic while the others did not damage boar spermatozoa. Thus, the other treatments may be used to produce profitable effects without adverse effects. Moreover, the addition of PGF2α at 5 mg/100 ml to sperm diluted in BTS may maintain sperm viability and motility better after 6 days of cooling, because significant differences were observed (P < 0.05) compared with control at the same time
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
peer-reviewed
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/25981
http://hdl.handle.net/10256/25981
url http://hdl.handle.net/10256/25981
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2007.08.008
info:eu-repo/semantics/altIdentifier/issn/0378-4320
info:eu-repo/semantics/altIdentifier/eissn/1873-2232
dc.rights.none.fl_str_mv Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv © Animal Reproduction Science, 2008, vol. 108, núm. 1-2, p. 180-195
Articles publicats (D-B)
Yeste Oliveras, Marc Briz González, Maria Dolors Pinart Nadal, Elisabeth Sancho Badell, Sílvia Garcia Gil, Núria Badia Brea, Maria Elena Bassols Casadevall, Judit Pruneda Sais, Anna Bussalleu Muntada, Eva Casas Roqueta, Isabel Bonet, Sergi 2008 Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time Animal Reproduction Science 108 1-2 180 195
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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