Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters

[EN] Live yeast cultures and yeast hydrolyzates can be used as rumen fermentation modifiers, but their effects and mode of action are different. Whereas the effects of live yeast cultures on rumen fermentation are well documented, yeast hydrolyzates have received much less attention. The influence o...

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Autores: Díaz Reyes, Alexey, Ranilla García, María José, Saro Higuera, Cristina, Tejido, María Luisa, Pérez Quintana, Manuel, Carro Travieso, María Dolores
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2017
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:dnet:buleria_____::cbf8fd2dba70ca4a3f0bae9955235600
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0377840117303115?via%3Dihub
https://hdl.handle.net/10612/27973
Access Level:acceso abierto
Palabra clave:Producción animal
Yeasts hydrolyzate
Rusitec
Rumen fermentation
ARISA
Batch cultures
3104 Producción Animal
3109 Ciencias veterinarias
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oai_identifier_str oai:dnet:buleria_____::cbf8fd2dba70ca4a3f0bae9955235600
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters
title Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters
spellingShingle Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters
Díaz Reyes, Alexey
Producción animal
Yeasts hydrolyzate
Rusitec
Rumen fermentation
ARISA
Batch cultures
3104 Producción Animal
3109 Ciencias veterinarias
title_short Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters
title_full Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters
title_fullStr Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters
title_full_unstemmed Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters
title_sort Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermenters
dc.creator.none.fl_str_mv Díaz Reyes, Alexey
Ranilla García, María José
Saro Higuera, Cristina
Tejido, María Luisa
Pérez Quintana, Manuel
Carro Travieso, María Dolores
author Díaz Reyes, Alexey
author_facet Díaz Reyes, Alexey
Ranilla García, María José
Saro Higuera, Cristina
Tejido, María Luisa
Pérez Quintana, Manuel
Carro Travieso, María Dolores
author_role author
author2 Ranilla García, María José
Saro Higuera, Cristina
Tejido, María Luisa
Pérez Quintana, Manuel
Carro Travieso, María Dolores
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Producción Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Producción animal
Yeasts hydrolyzate
Rusitec
Rumen fermentation
ARISA
Batch cultures
3104 Producción Animal
3109 Ciencias veterinarias
topic Producción animal
Yeasts hydrolyzate
Rusitec
Rumen fermentation
ARISA
Batch cultures
3104 Producción Animal
3109 Ciencias veterinarias
description [EN] Live yeast cultures and yeast hydrolyzates can be used as rumen fermentation modifiers, but their effects and mode of action are different. Whereas the effects of live yeast cultures on rumen fermentation are well documented, yeast hydrolyzates have received much less attention. The influence of a yeast hydrolyzate from Saccharomyces cerevisiae, obtained after ethanol production from sugarcane (YHS), on in vitro rumen fermentation was investigated using both batch cultures and Rusitec fermenters inoculated with ruminal fluid from sheep. Batch cultures (300 mg dry matter (DM)) with two mixed diets (AHC, 0.5:0.5 alfalfa hay:concentrate; BSC, 0.15:0.85 barley straw:concentrate) as substrate were supplemented with increasing doses of YHS (0, 3.3, 6.7, 10.0 and 13.3 ml/l) and incubated for 16.5 h at 39 °C. Supplementation of increasing amounts of YHS to AHC-cultures increased (P < 0.05) linearly total volatile fatty acid (VFA) production and butyrate molar proportion, and decreased (P < 0.001) acetate proportion and acetate: propionate ratio. In contrast, only subtle effects of YHS on NH3-N concentrations and molar proportions of isovalerate and caproate were observed for the BSC diet. Longer-term effects of YHS supplementation on rumen fermentation of AHC diet were investigated using four Rusitec fermenters in a cross-over experimental design with two 14-day incubation periods. Fermenters were given daily 30 g of diet DM, and in each period half of them were supplemented daily with 5 ml of YHS (10.0 ml/l) Supplementing with YHS did not affect (P > 0.05) total VFA production, lactate concentrations, DM and aNDFom disappearance or enzymatic activities (amylase, xylanase and carboxymethylcellulase). Compared with the unsupplemented fermenters, YHS treatment increased (P < 0.001) NH3-N concentrations and molar proportions of propionate and butyrate at the expense of acetate, and decreased (P < 0.001) acetate:propionate ratio. In addition, YHS supplementation tended (P < 0.07) to reduce CH4/total VFA ratio and to increase microbial growth in the liquid phase of the fermenters. The automated ribosomal intergenic spacer analysis (ARISA) of samples taken on days 3, 8 and 14 of incubation from solid and liquid content of fermenters revealed that YHS supplementation increased (P < 0.02) bacterial diversity in the liquid phase and tended to increase (P < 0.08) it in the solid phase. The results indicate that YHS at a dose of 10 ml/l may be a useful dietary additive for ruminants, because it promoted a shift in fermentation toward propionate production, reduced the CH4/total VFA ratio and increased microbial growth with a 50:50 alfalfa hay:concentrate diet.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0377840117303115?via%3Dihub
https://hdl.handle.net/10612/27973
url https://www.sciencedirect.com/science/article/pii/S0377840117303115?via%3Dihub
https://hdl.handle.net/10612/27973
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ Ministerio de Ciencia e Innovación/ Programa Nacional de Investigación Fundamental / AGL2008-04707-C02-02
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Influence of increasing doses of a yeast hydrolyzate obtained from sugarcane processing on in vitro rumen fermentation of two different diets and bacterial diversity in batch cultures and Rusitec fermentersDíaz Reyes, AlexeyRanilla García, María JoséSaro Higuera, CristinaTejido, María LuisaPérez Quintana, ManuelCarro Travieso, María DoloresProducción animalYeasts hydrolyzateRusitecRumen fermentationARISABatch cultures3104 Producción Animal3109 Ciencias veterinarias[EN] Live yeast cultures and yeast hydrolyzates can be used as rumen fermentation modifiers, but their effects and mode of action are different. Whereas the effects of live yeast cultures on rumen fermentation are well documented, yeast hydrolyzates have received much less attention. The influence of a yeast hydrolyzate from Saccharomyces cerevisiae, obtained after ethanol production from sugarcane (YHS), on in vitro rumen fermentation was investigated using both batch cultures and Rusitec fermenters inoculated with ruminal fluid from sheep. Batch cultures (300 mg dry matter (DM)) with two mixed diets (AHC, 0.5:0.5 alfalfa hay:concentrate; BSC, 0.15:0.85 barley straw:concentrate) as substrate were supplemented with increasing doses of YHS (0, 3.3, 6.7, 10.0 and 13.3 ml/l) and incubated for 16.5 h at 39 °C. Supplementation of increasing amounts of YHS to AHC-cultures increased (P < 0.05) linearly total volatile fatty acid (VFA) production and butyrate molar proportion, and decreased (P < 0.001) acetate proportion and acetate: propionate ratio. In contrast, only subtle effects of YHS on NH3-N concentrations and molar proportions of isovalerate and caproate were observed for the BSC diet. Longer-term effects of YHS supplementation on rumen fermentation of AHC diet were investigated using four Rusitec fermenters in a cross-over experimental design with two 14-day incubation periods. Fermenters were given daily 30 g of diet DM, and in each period half of them were supplemented daily with 5 ml of YHS (10.0 ml/l) Supplementing with YHS did not affect (P > 0.05) total VFA production, lactate concentrations, DM and aNDFom disappearance or enzymatic activities (amylase, xylanase and carboxymethylcellulase). Compared with the unsupplemented fermenters, YHS treatment increased (P < 0.001) NH3-N concentrations and molar proportions of propionate and butyrate at the expense of acetate, and decreased (P < 0.001) acetate:propionate ratio. In addition, YHS supplementation tended (P < 0.07) to reduce CH4/total VFA ratio and to increase microbial growth in the liquid phase of the fermenters. The automated ribosomal intergenic spacer analysis (ARISA) of samples taken on days 3, 8 and 14 of incubation from solid and liquid content of fermenters revealed that YHS supplementation increased (P < 0.02) bacterial diversity in the liquid phase and tended to increase (P < 0.08) it in the solid phase. The results indicate that YHS at a dose of 10 ml/l may be a useful dietary additive for ruminants, because it promoted a shift in fermentation toward propionate production, reduced the CH4/total VFA ratio and increased microbial growth with a 50:50 alfalfa hay:concentrate diet.SIFunding was provided by the Spanish Ministry of Economy and Competitiveness (Project AGL2008-04707-C02-02 and Acción Integrada AIB2010NZ-00190). A. Díaz gratefully acknowledges the receipt of grants from the Spanish AECID and the Junta de Castilla y León. C. Saro gratefully acknowledges the receipt of scholarship from the Spanish Ministry of Education and Science (AP2006-03049).ElsevierProducción AnimalFacultad de Veterinaria2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttps://www.sciencedirect.com/science/article/pii/S0377840117303115?via%3Dihubhttps://hdl.handle.net/10612/27973reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/ Ministerio de Ciencia e Innovación/ Programa Nacional de Investigación Fundamental / AGL2008-04707-C02-02http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:dnet:buleria_____::cbf8fd2dba70ca4a3f0bae99552356002026-06-24T12:43:27Z
score 15.812455

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