Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities

The recovery of polysaccharides (PS) from red grape marc and white grape pomace by enzymatic degradation of their cell walls is an interesting green extraction technique that preserves the structure and bioactivity of PS. The type and dose of enzyme, and the liquid/solid (L/S) ratio in PS extraction...

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Detalles Bibliográficos
Autores: Garaigordobil, Ekhiñe, Martínez-Lapuente, Leticia, Guadalupe, Zenaida, Pérez-Magariño, Silvia, Ayestarán, Belén
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/383618
Acceso en línea:http://hdl.handle.net/10261/383618
https://api.elsevier.com/content/abstract/scopus_id/85216099760
Access Level:acceso abierto
Palabra clave:By-products
Efficiency
Enzyme-assisted extraction
Grape marc/pomace
Grape polysaccharides from grapes
Molecular weight distribution of polysaccharides
Purity
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spelling Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different ActivitiesGaraigordobil, EkhiñeMartínez-Lapuente, LeticiaGuadalupe, ZenaidaPérez-Magariño, SilviaAyestarán, BelénBy-productsEfficiencyEnzyme-assisted extractionGrape marc/pomaceGrape polysaccharides from grapesMolecular weight distribution of polysaccharidesPurityThe recovery of polysaccharides (PS) from red grape marc and white grape pomace by enzymatic degradation of their cell walls is an interesting green extraction technique that preserves the structure and bioactivity of PS. The type and dose of enzyme, and the liquid/solid (L/S) ratio in PS extraction were studied using four commercial enzymes. Four different doses per enzyme were used, with tartaric acid as solvent and L/S ratios of 1.3/1 and 4/1 for 24 h at 20 °C, compared with a control. The highest dose of enzyme E1, polygalacturonase + pectin lyase + pectin-methyl-esterase (with the highest activity) was the most effective in the degradation of high and medium molecular weight PS. At the lower L/S ratio, the fact that the highest dose of E1 degraded a higher percentage of high and medium molecular weight PS in the marc was explained by the difference in cell wall deconstruction between pomace and marc. The highest total PS purity was achieved in pomace with E1 at the maximum dose in both ratios, and in marc at the 1.3/1 ratio. The extraction efficiency of total PS was low for all enzymes. In the future, extraction with E1 combined with other green extraction techniques will be studied.Project PID2021-123361OR-C22 funded by MICIU/AEI/10.13039/501100011033 and by FEDER, EU.Peer reviewedMultidisciplinary Digital Publishing InstituteAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)European CommissionMartínez-Lapuente, Leticia [0000-0001-7064-8257]Guadalupe, Zenaida [0000-0002-6490-1428]Pérez-Magariño, Silvia [0000-0002-0880-7154]Ayestarán, Belén [0000-0002-2903-1332]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/383618https://api.elsevier.com/content/abstract/scopus_id/85216099760reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.3390/molecules30020213https://doi.org/10.3390/molecules30020213Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3836182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities
title Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities
spellingShingle Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities
Garaigordobil, Ekhiñe
By-products
Efficiency
Enzyme-assisted extraction
Grape marc/pomace
Grape polysaccharides from grapes
Molecular weight distribution of polysaccharides
Purity
title_short Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities
title_full Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities
title_fullStr Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities
title_full_unstemmed Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities
title_sort Recovery of Polysaccharides from Red Grape Marc and White Grape Pomace by Degradation of Cell Walls by Enzymes with Different Activities
dc.creator.none.fl_str_mv Garaigordobil, Ekhiñe
Martínez-Lapuente, Leticia
Guadalupe, Zenaida
Pérez-Magariño, Silvia
Ayestarán, Belén
author Garaigordobil, Ekhiñe
author_facet Garaigordobil, Ekhiñe
Martínez-Lapuente, Leticia
Guadalupe, Zenaida
Pérez-Magariño, Silvia
Ayestarán, Belén
author_role author
author2 Martínez-Lapuente, Leticia
Guadalupe, Zenaida
Pérez-Magariño, Silvia
Ayestarán, Belén
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Martínez-Lapuente, Leticia [0000-0001-7064-8257]
Guadalupe, Zenaida [0000-0002-6490-1428]
Pérez-Magariño, Silvia [0000-0002-0880-7154]
Ayestarán, Belén [0000-0002-2903-1332]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv By-products
Efficiency
Enzyme-assisted extraction
Grape marc/pomace
Grape polysaccharides from grapes
Molecular weight distribution of polysaccharides
Purity
topic By-products
Efficiency
Enzyme-assisted extraction
Grape marc/pomace
Grape polysaccharides from grapes
Molecular weight distribution of polysaccharides
Purity
description The recovery of polysaccharides (PS) from red grape marc and white grape pomace by enzymatic degradation of their cell walls is an interesting green extraction technique that preserves the structure and bioactivity of PS. The type and dose of enzyme, and the liquid/solid (L/S) ratio in PS extraction were studied using four commercial enzymes. Four different doses per enzyme were used, with tartaric acid as solvent and L/S ratios of 1.3/1 and 4/1 for 24 h at 20 °C, compared with a control. The highest dose of enzyme E1, polygalacturonase + pectin lyase + pectin-methyl-esterase (with the highest activity) was the most effective in the degradation of high and medium molecular weight PS. At the lower L/S ratio, the fact that the highest dose of E1 degraded a higher percentage of high and medium molecular weight PS in the marc was explained by the difference in cell wall deconstruction between pomace and marc. The highest total PS purity was achieved in pomace with E1 at the maximum dose in both ratios, and in marc at the 1.3/1 ratio. The extraction efficiency of total PS was low for all enzymes. In the future, extraction with E1 combined with other green extraction techniques will be studied.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/383618
https://api.elsevier.com/content/abstract/scopus_id/85216099760
url http://hdl.handle.net/10261/383618
https://api.elsevier.com/content/abstract/scopus_id/85216099760
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.3390/molecules30020213
https://doi.org/10.3390/molecules30020213
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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