Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes

Producción Científica

Detalhes bibliográficos
Autores: Eugui, Daniel, Velasco, Pablo, Abril Urías, Patricia, Escobar, Carolina, Gómez Torres, Óscar, Caballero, Sara, Poveda Arias, Jorge
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Universidad de Valladolid
Repositório:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/59561
Acesso em linha:https://doi.org/10.1016/j.indcrop.2023.116841
https://uvadoc.uva.es/handle/10324/59561
Access Level:Acceso aberto
Palavra-chave:Gestión forestal
Plantas - Enfermedades y plagas
Sustainable agriculture
Isothiocyanates
Brassicaceae
Agricultura sostenible
Isotiocianatos
Brasicáceas
3106 Ciencia Forestal
2417.09 Fitopatología
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spelling Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodesEugui, DanielVelasco, PabloAbril Urías, PatriciaEscobar, CarolinaGómez Torres, ÓscarCaballero, SaraPoveda Arias, JorgeGestión forestalPlantas - Enfermedades y plagasSustainable agricultureIsothiocyanatesBrassicaceaeAgricultura sostenibleIsotiocianatosBrasicáceas3106 Ciencia Forestal2417.09 FitopatologíaProducción CientíficaThis study aimed to standarize a protocol for obtaining a bioactive extract from broccoli (Brassica oleracea var. italica) crop residues, that is suitable for application on an industrial scale and effective in reducing plant disease incidence. For this purpose, the influence of several extraction factors in the glucosinolate (GSL) content was studied with leaves collected from two conventional broccoli fields and two organic broccoli fields. The analysis showed that lyophilization had no influence on the GSL content. Storage of plant material under two different temperatures (− 20ºC and − 80ºC) had no influence on the GSLs content of the extracts. Phytotoxicity of the extracts was studied with six different plant seeds, and also cytotoxicity was determined with human liver cells in vitro. The extracts were phytotoxic at dilutions above 10%, while cell toxicity was low. Extracts concentrations of 0.1%, 1% and 2% were tested in vitro against eight plant pathogenic fungi and two oomycetes in solid and in liquid media. The extracts reduced the growth of several plant pathogenic fungi at 2% dilution by up to 38.37% against Alternaria alternata and up to 46.55% against Sclerotinia sclerotiorum. When combined with myrosinase enzyme the effect of the extracts was enhanced, reaching inhibition values of 67.06% against A. alternata in solid medium and 68.52% against Rhizoctonia solani in liquid medium. In contrast, the same extracts increased the growth of the plant pathogenic oomycetes Pythium ultimum and Phytophthora cactorum. The effect of the same extracts in the free leaving larvae, J2s, of the plant-parasitic nematode (PPN) Meloidogyne javanica was not obvious. Minor significant differences were obtained but with no clear dose-response in nematode mortality, and no inhibition of eggs hatching was observed. These results show the industrial potential of using broccoli residues to obtain extracts with biopesticide activity against plant pathogenic fungi.Agencia Estatal de Investigación (DIN2018-009852)Elsevier2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.indcrop.2023.116841https://uvadoc.uva.es/handle/10324/59561reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/pii/S0926669023006064info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/595612026-06-13T12:44:47Z
dc.title.none.fl_str_mv Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes
title Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes
spellingShingle Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes
Eugui, Daniel
Gestión forestal
Plantas - Enfermedades y plagas
Sustainable agriculture
Isothiocyanates
Brassicaceae
Agricultura sostenible
Isotiocianatos
Brasicáceas
3106 Ciencia Forestal
2417.09 Fitopatología
title_short Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes
title_full Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes
title_fullStr Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes
title_full_unstemmed Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes
title_sort Glucosinolate-extracts from residues of conventional and organic cultivated broccoli leaves (Brassica oleracea var. italica) as potential industrially-scalable efficient biopesticides against fungi, oomycetes and plant parasitic nematodes
dc.creator.none.fl_str_mv Eugui, Daniel
Velasco, Pablo
Abril Urías, Patricia
Escobar, Carolina
Gómez Torres, Óscar
Caballero, Sara
Poveda Arias, Jorge
author Eugui, Daniel
author_facet Eugui, Daniel
Velasco, Pablo
Abril Urías, Patricia
Escobar, Carolina
Gómez Torres, Óscar
Caballero, Sara
Poveda Arias, Jorge
author_role author
author2 Velasco, Pablo
Abril Urías, Patricia
Escobar, Carolina
Gómez Torres, Óscar
Caballero, Sara
Poveda Arias, Jorge
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Gestión forestal
Plantas - Enfermedades y plagas
Sustainable agriculture
Isothiocyanates
Brassicaceae
Agricultura sostenible
Isotiocianatos
Brasicáceas
3106 Ciencia Forestal
2417.09 Fitopatología
topic Gestión forestal
Plantas - Enfermedades y plagas
Sustainable agriculture
Isothiocyanates
Brassicaceae
Agricultura sostenible
Isotiocianatos
Brasicáceas
3106 Ciencia Forestal
2417.09 Fitopatología
description Producción Científica
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.indcrop.2023.116841
https://uvadoc.uva.es/handle/10324/59561
url https://doi.org/10.1016/j.indcrop.2023.116841
https://uvadoc.uva.es/handle/10324/59561
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0926669023006064
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.228081

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