Microglial cells in astroglial cultures: a cautionary note

Primary rodent astroglial-enriched cultures are the most popular model to study astroglial biology in vitro. From the original methods described in the 1970's a great number of minor modifications have been incorporated into these protocols by different laboratories. These protocols result in c...

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Autor: Saura Martí, Josep
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/7284
Acceso en línea:https://hdl.handle.net/2445/7284
Access Level:acceso abierto
Palabra clave:Diferenciació cel·lular
Cultius cel·lulars
Cell culture techniques
Microglia
Cell differentiation
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spelling Microglial cells in astroglial cultures: a cautionary noteSaura Martí, JosepDiferenciació cel·lularCultius cel·lularsCell culture techniquesMicrogliaCell differentiationPrimary rodent astroglial-enriched cultures are the most popular model to study astroglial biology in vitro. From the original methods described in the 1970's a great number of minor modifications have been incorporated into these protocols by different laboratories. These protocols result in cultures in which the astrocyte is the predominant cell type, but astrocytes are never 100% of cells in these preparations. The aim of this review is to bring attention to the presence of microglia in astroglial cultures because, in my opinion, the proportion of and the role that microglial cells play in astroglial cultures are often underestimated. The main problem with ignoring microglia in these cultures is that relatively minor amounts of microglia can be responsible for effects observed on cultures in which the astrocyte is the most abundant cell type. If the relative contributions of astrocytes and microglia are not properly assessed an observed effect can be erroneously attributed to the astrocytes. In order to illustrate this point the case of NO production in activated astroglial-enriched cultures is examined. Lipopolysaccharide (LPS) induces nitric oxide (NO) production in astroglial-enriched cultures and this effect is very often attributed to astrocytes. However, a careful review of the published data suggests that LPS-induced NO production in rodent astroglial-enriched cultures is likely to be mainly microglial in origin. This review considers cell culture protocol factors that can affect the proportion of microglial cells in astroglial cultures, strategies to minimize the proportion of microglia in these cultures, and specific markers that allow the determination of such microglial proportions.BioMed Central2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/7284Articles publicats en revistes (Ciències Fisiològiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a http://dx.doi.org/10.1186/1742-2094-4-26Journal of Neuroinflammation, 2007, vol. 4, núm. 26http://dx.doi.org/10.1186/1742-2094-4-26cc-by, (c) Saura et al., 2007http://creativecommons.org/licenses/by/2.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/72842026-05-27T06:46:51Z
dc.title.none.fl_str_mv Microglial cells in astroglial cultures: a cautionary note
title Microglial cells in astroglial cultures: a cautionary note
spellingShingle Microglial cells in astroglial cultures: a cautionary note
Saura Martí, Josep
Diferenciació cel·lular
Cultius cel·lulars
Cell culture techniques
Microglia
Cell differentiation
title_short Microglial cells in astroglial cultures: a cautionary note
title_full Microglial cells in astroglial cultures: a cautionary note
title_fullStr Microglial cells in astroglial cultures: a cautionary note
title_full_unstemmed Microglial cells in astroglial cultures: a cautionary note
title_sort Microglial cells in astroglial cultures: a cautionary note
dc.creator.none.fl_str_mv Saura Martí, Josep
author Saura Martí, Josep
author_facet Saura Martí, Josep
author_role author
dc.subject.none.fl_str_mv Diferenciació cel·lular
Cultius cel·lulars
Cell culture techniques
Microglia
Cell differentiation
topic Diferenciació cel·lular
Cultius cel·lulars
Cell culture techniques
Microglia
Cell differentiation
description Primary rodent astroglial-enriched cultures are the most popular model to study astroglial biology in vitro. From the original methods described in the 1970's a great number of minor modifications have been incorporated into these protocols by different laboratories. These protocols result in cultures in which the astrocyte is the predominant cell type, but astrocytes are never 100% of cells in these preparations. The aim of this review is to bring attention to the presence of microglia in astroglial cultures because, in my opinion, the proportion of and the role that microglial cells play in astroglial cultures are often underestimated. The main problem with ignoring microglia in these cultures is that relatively minor amounts of microglia can be responsible for effects observed on cultures in which the astrocyte is the most abundant cell type. If the relative contributions of astrocytes and microglia are not properly assessed an observed effect can be erroneously attributed to the astrocytes. In order to illustrate this point the case of NO production in activated astroglial-enriched cultures is examined. Lipopolysaccharide (LPS) induces nitric oxide (NO) production in astroglial-enriched cultures and this effect is very often attributed to astrocytes. However, a careful review of the published data suggests that LPS-induced NO production in rodent astroglial-enriched cultures is likely to be mainly microglial in origin. This review considers cell culture protocol factors that can affect the proportion of microglial cells in astroglial cultures, strategies to minimize the proportion of microglia in these cultures, and specific markers that allow the determination of such microglial proportions.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/7284
url https://hdl.handle.net/2445/7284
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a http://dx.doi.org/10.1186/1742-2094-4-26
Journal of Neuroinflammation, 2007, vol. 4, núm. 26
http://dx.doi.org/10.1186/1742-2094-4-26
dc.rights.none.fl_str_mv cc-by, (c) Saura et al., 2007
http://creativecommons.org/licenses/by/2.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by, (c) Saura et al., 2007
http://creativecommons.org/licenses/by/2.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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