A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology

Endomyocardial biopsies are the gold standard for surveillance of graft rejection following heart transplantation, and are assessed by classical histopathology using a limited number of previously stained slices from several biopsies. Synchrotron propagation-based X-ray phase contrast imaging is a n...

Descripción completa

Detalles Bibliográficos
Autores: Planinc, Ivo, Ilic, Ivana, Dejea i Velardo, Hèctor, García-Canadilla, Patricia, Gasparovic, Hrvoje, Jurin, Hrvoje, Milicic, Davor, Skoric, Bosko, Stampanoni, Marco, Bijnens, Bart, Bonnin, Anne, Cikes, Maja
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/71551
Acceso en línea:http://hdl.handle.net/10230/71551
http://dx.doi.org/10.3389/ti.2023.11046
Access Level:acceso abierto
Palabra clave:Heart transplantation
Synchrotron
Histopathology
Graft rejection
X-ray phase contrast imaging
id ES_7f4d518eee764d955a117db7d1a91fe9
oai_identifier_str oai:repositori.upf.edu:10230/71551
network_acronym_str ES
network_name_str España
repository_id_str
spelling A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathologyPlaninc, IvoIlic, IvanaDejea i Velardo, HèctorGarcía-Canadilla, PatriciaGasparovic, HrvojeJurin, HrvojeMilicic, DavorSkoric, BoskoStampanoni, MarcoBijnens, BartBonnin, AnneCikes, MajaHeart transplantationSynchrotronHistopathologyGraft rejectionX-ray phase contrast imagingEndomyocardial biopsies are the gold standard for surveillance of graft rejection following heart transplantation, and are assessed by classical histopathology using a limited number of previously stained slices from several biopsies. Synchrotron propagation-based X-ray phase contrast imaging is a non-destructive method to image biological samples without tissue preparation, enabling virtual 2D and 3D histopathology. We aimed to show the feasibility of this method to assess acute cellular rejection and its agreement to classical histopathology. Right ventricular biopsies were sampled from 23 heart transplantation recipients (20 males, mean age 54±14 years) as part of standard follow-up. The clinical diagnosis of potential rejection was made using classical histopathology. One additional study sample was harvested and imaged by X-ray phase contrast imaging, producing 3D datasets with 0.65 μm pixel size, and up to 4,320 images per sample. An experienced pathologist graded both histopathological and X-ray phase contrast images in a blinded fashion. The agreement between methods was assessed by weighted kappa, showing substantial agreement (kappa up to 0.80, p < 0.01) between X-ray phase contrast imaging and classical histopathology. X-ray phase contrast imaging does not require tissue processing, allows thorough analysis of a full myocardial sample and allows identification of acute cellular rejection.This project was supported by the grant #2017-303 of the Strategic Focal Area “Personalized Health and Related Technologies (PHRT)” of the ETH Domain, the Spanish Ministry of Economy and Competitiveness (grant TIN2014-52923-R, the Maria de Maeztu Units of Excellence Programme MDM-2015-0502) and Fondo Europeo de Desarrollo Regional (FEDER). This project was supported by the Scientific Support programme of the University of Zagreb for year 2018: “Analysis of myocardial structure in heart failure with X-ray phase-contrast cardiac imaging.” This project was supported by the Scientific Support programme of the University of Zagreb for year 2019: “The specificities of myocardial structure based on heart failure aetiology—an X-ray phase contrast cardiac imaging analysis.” This project was supported by the Croatian Science Foundation through the project IP-2020-02-5572 [New Paradigm in Graft Rejection Detection in Heart Transplantation Recipients: Diagnostic Utility of Synchrotron X-ray Phase Contrast Imaging (GRAFT-XPCI)].Frontiers202520252023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/71551http://dx.doi.org/10.3389/ti.2023.11046reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésTransplant International. 2023 Jan 24;36:11046info:eu-repo/grantAgreement/ES/1PE/TIN2014-52923-R© 2023 Planinc, Ilic, Dejea, Garcia-Canadilla, Gasparovic, Jurin, Milicic, Skoric, Stampanoni, Bijnens, Bonnin and Cikes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/715512026-06-12T07:21:37Z
dc.title.none.fl_str_mv A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
title A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
spellingShingle A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
Planinc, Ivo
Heart transplantation
Synchrotron
Histopathology
Graft rejection
X-ray phase contrast imaging
title_short A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
title_full A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
title_fullStr A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
title_full_unstemmed A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
title_sort A novel three-dimensional approach towards evaluating endomyocardial biopsies for follow-up after heart transplantation: x-ray phase contrast imaging and its agreement with classical histopathology
dc.creator.none.fl_str_mv Planinc, Ivo
Ilic, Ivana
Dejea i Velardo, Hèctor
García-Canadilla, Patricia
Gasparovic, Hrvoje
Jurin, Hrvoje
Milicic, Davor
Skoric, Bosko
Stampanoni, Marco
Bijnens, Bart
Bonnin, Anne
Cikes, Maja
author Planinc, Ivo
author_facet Planinc, Ivo
Ilic, Ivana
Dejea i Velardo, Hèctor
García-Canadilla, Patricia
Gasparovic, Hrvoje
Jurin, Hrvoje
Milicic, Davor
Skoric, Bosko
Stampanoni, Marco
Bijnens, Bart
Bonnin, Anne
Cikes, Maja
author_role author
author2 Ilic, Ivana
Dejea i Velardo, Hèctor
García-Canadilla, Patricia
Gasparovic, Hrvoje
Jurin, Hrvoje
Milicic, Davor
Skoric, Bosko
Stampanoni, Marco
Bijnens, Bart
Bonnin, Anne
Cikes, Maja
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Heart transplantation
Synchrotron
Histopathology
Graft rejection
X-ray phase contrast imaging
topic Heart transplantation
Synchrotron
Histopathology
Graft rejection
X-ray phase contrast imaging
description Endomyocardial biopsies are the gold standard for surveillance of graft rejection following heart transplantation, and are assessed by classical histopathology using a limited number of previously stained slices from several biopsies. Synchrotron propagation-based X-ray phase contrast imaging is a non-destructive method to image biological samples without tissue preparation, enabling virtual 2D and 3D histopathology. We aimed to show the feasibility of this method to assess acute cellular rejection and its agreement to classical histopathology. Right ventricular biopsies were sampled from 23 heart transplantation recipients (20 males, mean age 54±14 years) as part of standard follow-up. The clinical diagnosis of potential rejection was made using classical histopathology. One additional study sample was harvested and imaged by X-ray phase contrast imaging, producing 3D datasets with 0.65 μm pixel size, and up to 4,320 images per sample. An experienced pathologist graded both histopathological and X-ray phase contrast images in a blinded fashion. The agreement between methods was assessed by weighted kappa, showing substantial agreement (kappa up to 0.80, p < 0.01) between X-ray phase contrast imaging and classical histopathology. X-ray phase contrast imaging does not require tissue processing, allows thorough analysis of a full myocardial sample and allows identification of acute cellular rejection.
publishDate 2023
dc.date.none.fl_str_mv 2023
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/71551
http://dx.doi.org/10.3389/ti.2023.11046
url http://hdl.handle.net/10230/71551
http://dx.doi.org/10.3389/ti.2023.11046
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Transplant International. 2023 Jan 24;36:11046
info:eu-repo/grantAgreement/ES/1PE/TIN2014-52923-R
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411813772230656
score 15.228081

Similares en LA Referencia