Efecto del Taxol sobre la autofagia en células tumorales de colon
[EN]Paclitaxel is an anticancer drug used in the treatment of ovarian, breast, non small cell lung, and Kaposi’s sarcoma associated with AIDS. Its mechanism of action is based on blocking β-tubulin subunit of microtubules, decreasing depolymerisation and stabilizing them; stopping the cell cycle at...
| Autores: | , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2017 |
| País: | España |
| Recursos: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/133708 |
| Acesso em linha: | http://hdl.handle.net/10366/133708 |
| Access Level: | acceso abierto |
| Palavra-chave: | paclitaxel cáncer colon autofagia p62 LC3-II Beclin 1 autophagy |
| Resumo: | [EN]Paclitaxel is an anticancer drug used in the treatment of ovarian, breast, non small cell lung, and Kaposi’s sarcoma associated with AIDS. Its mechanism of action is based on blocking β-tubulin subunit of microtubules, decreasing depolymerisation and stabilizing them; stopping the cell cycle at the G2 / M phase leading to apoptosis. Furthermore, the autophagy, in which process microtubules are very important, is a set of catabolic processes crucial for the maintenance of cell viability; but also is a programmed cell death. The target of this article is to study the drug cytotoxicity and its effect on 6 autophagy related proteins: P62, PKC ζ, mTOR, TRAF6, LC3 and Beclin 1; on two colon cancer cell lines: HT29 and HCT-116. The techniques used were the MTT and Western Blot tests. Our results indicate that paclitaxel inhibits the growth of both lines at 10 nM; and increases the expression of Beclin 1, LC3-II and p62 proteins, which indicates an induction of autophagy, and a later block of autolisosoma. In conclusion, we can say that paclitaxel blocks autophagy. Key words: paclitaxel; cáncer; colon; autophagy; p62; LC3-II; Beclin 1. |
|---|