Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain
Infections caused by carbapenem-resistant Acinetobacter baumannii are a global threat causing a high number of fatal infections. This microorganism can also easily acquire antibiotic resistance determinants, making the treatment of infections a big challenge, and has the ability to persist in the ho...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad del País Vasco |
| Repositorio: | Addi. Archivo Digital para la Docencia y la Investigación |
| OAI Identifier: | oai:addi.ehu.eus:10810/66603 |
| Acceso en línea: | http://hdl.handle.net/10810/66603 |
| Access Level: | acceso abierto |
| Palabra clave: | Acinetobacter baumannii carbapenem resistance hospital outbreaks sequencing plasmid persistence |
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Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern SpainAranzamendi, MaitaneXanthopoulou, KyriakiSánchez Urtaza, SandraBurgwinkel, TessaArazo del Pino, RocíoLucaßen, KaiPérez Vázquez, M.Oteo Iglesias, JesúsSota, MercedesMarimón Ortiz de Zarate, José MaríaSeifert, HaraldHiggins, Paul G.Gallego Andrés, LucíaAcinetobacter baumanniicarbapenem resistancehospital outbreakssequencingplasmidpersistenceInfections caused by carbapenem-resistant Acinetobacter baumannii are a global threat causing a high number of fatal infections. This microorganism can also easily acquire antibiotic resistance determinants, making the treatment of infections a big challenge, and has the ability to persist in the hospital environment under a wide range of conditions. The objective of this work was to study the molecular epidemiology and genetic characteristics of two blaOXA24/40 Acinetobacter baumannii outbreaks (2009 and 2020-21) at a tertiary hospital in Northern Spain. Thirty-six isolates were investigated and genotypically screened by Whole Genome Sequencing to analyse the resistome and virulome. Isolates were resistant to carbapenems, aminoglycosides and fluoroquinolones. Multi-Locus Sequence Typing analysis identified that Outbreak 1 was mainly produced by isolates belonging to ST3Pas/ST106Oxf (IC3) containing blaOXA24/40, blaOXA71 and blaADC119. Outbreak 2 isolates were exclusively ST2Pas/ST801Oxf (IC2) blaOXA24/40, blaOXA66 and blaADC30, the same genotype seen in two isolates from 2009. Virulome analysis showed that IC2 isolates contained genes for capsular polysaccharide KL32 and lipooligosacharide OCL5. A 8.9 Kb plasmid encoding the blaOXA24/40 gene was common in all isolates. The persistance over time of a virulent IC2 clone highlights the need of active surveillance to control its spread.This research was funded by the Microbiology Department, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group, Biogipuzkoa Health Research Institute and the DEPARTMENT OF EDUCATION OF THE BASQUE GOVERNMENT (Research Groups of the Basque University System 2021), grant number Group IT1578-22, GIC21/18. SS-U is funded by the Basque Government’s Postdoctoral Fellowship (2023–2026).MDPI2024202420242024info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/66603reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.mdpi.com/1422-0067/25/4/2333info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/es/© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).oai:addi.ehu.eus:10810/666032026-06-18T09:23:17Z |
| dc.title.none.fl_str_mv |
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain |
| title |
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain |
| spellingShingle |
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain Aranzamendi, Maitane Acinetobacter baumannii carbapenem resistance hospital outbreaks sequencing plasmid persistence |
| title_short |
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain |
| title_full |
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain |
| title_fullStr |
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain |
| title_full_unstemmed |
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain |
| title_sort |
Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40 Acinetobacter baumannii Clone in a Tertiary Hospital in Northern Spain |
| dc.creator.none.fl_str_mv |
Aranzamendi, Maitane Xanthopoulou, Kyriaki Sánchez Urtaza, Sandra Burgwinkel, Tessa Arazo del Pino, Rocío Lucaßen, Kai Pérez Vázquez, M. Oteo Iglesias, Jesús Sota, Mercedes Marimón Ortiz de Zarate, José María Seifert, Harald Higgins, Paul G. Gallego Andrés, Lucía |
| author |
Aranzamendi, Maitane |
| author_facet |
Aranzamendi, Maitane Xanthopoulou, Kyriaki Sánchez Urtaza, Sandra Burgwinkel, Tessa Arazo del Pino, Rocío Lucaßen, Kai Pérez Vázquez, M. Oteo Iglesias, Jesús Sota, Mercedes Marimón Ortiz de Zarate, José María Seifert, Harald Higgins, Paul G. Gallego Andrés, Lucía |
| author_role |
author |
| author2 |
Xanthopoulou, Kyriaki Sánchez Urtaza, Sandra Burgwinkel, Tessa Arazo del Pino, Rocío Lucaßen, Kai Pérez Vázquez, M. Oteo Iglesias, Jesús Sota, Mercedes Marimón Ortiz de Zarate, José María Seifert, Harald Higgins, Paul G. Gallego Andrés, Lucía |
| author2_role |
author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Acinetobacter baumannii carbapenem resistance hospital outbreaks sequencing plasmid persistence |
| topic |
Acinetobacter baumannii carbapenem resistance hospital outbreaks sequencing plasmid persistence |
| description |
Infections caused by carbapenem-resistant Acinetobacter baumannii are a global threat causing a high number of fatal infections. This microorganism can also easily acquire antibiotic resistance determinants, making the treatment of infections a big challenge, and has the ability to persist in the hospital environment under a wide range of conditions. The objective of this work was to study the molecular epidemiology and genetic characteristics of two blaOXA24/40 Acinetobacter baumannii outbreaks (2009 and 2020-21) at a tertiary hospital in Northern Spain. Thirty-six isolates were investigated and genotypically screened by Whole Genome Sequencing to analyse the resistome and virulome. Isolates were resistant to carbapenems, aminoglycosides and fluoroquinolones. Multi-Locus Sequence Typing analysis identified that Outbreak 1 was mainly produced by isolates belonging to ST3Pas/ST106Oxf (IC3) containing blaOXA24/40, blaOXA71 and blaADC119. Outbreak 2 isolates were exclusively ST2Pas/ST801Oxf (IC2) blaOXA24/40, blaOXA66 and blaADC30, the same genotype seen in two isolates from 2009. Virulome analysis showed that IC2 isolates contained genes for capsular polysaccharide KL32 and lipooligosacharide OCL5. A 8.9 Kb plasmid encoding the blaOXA24/40 gene was common in all isolates. The persistance over time of a virulent IC2 clone highlights the need of active surveillance to control its spread. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024 2024 2024 |
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info:eu-repo/semantics/article |
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article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10810/66603 |
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http://hdl.handle.net/10810/66603 |
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Inglés |
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Inglés |
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https://www.mdpi.com/1422-0067/25/4/2333 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/es/ |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/es/ |
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application/pdf |
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MDPI |
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MDPI |
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reponame:Addi. Archivo Digital para la Docencia y la Investigación instname:Universidad del País Vasco |
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