In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani

Flavonolignans from the seeds of the milk thistle (Silybum marianum) have been extensively used in folk medicine for centuries. Confirmation of their properties as hepatoprotective, antioxidant and anticancer has been obtained using standardized extracts and purified flavonolignans. Information on t...

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Detalles Bibliográficos
Autores: Olías Molero, Ana Isabel, Jiménez Antón, María Dolores, Biedermann, David, Corral Caridad, María Jesús, Alunda Rodríguez, José María
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/12525
Acceso en línea:https://hdl.handle.net/20.500.14352/12525
Access Level:acceso abierto
Palabra clave:leishmaniasis
Leishmania infantum
L. donovani
silybin
dehydrosilybin
dehydroisosilybin
amphotericin
paromomycin
SbIII
Patología veterinaria
3109.07 Patología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/12525
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repository_id_str
spelling In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovaniOlías Molero, Ana IsabelJiménez Antón, María DoloresBiedermann, DavidCorral Caridad, María JesúsAlunda Rodríguez, José MaríaleishmaniasisLeishmania infantumL. donovanisilybindehydrosilybindehydroisosilybinamphotericinparomomycinSbIIIPatología veterinaria3109.07 PatologíaFlavonolignans from the seeds of the milk thistle (Silybum marianum) have been extensively used in folk medicine for centuries. Confirmation of their properties as hepatoprotective, antioxidant and anticancer has been obtained using standardized extracts and purified flavonolignans. Information on their potential effect on Leishmania is very scarce. We have investigated the effect of silymarin, silybin and related flavonolignans on the multiplication of promastigotes in vitro and ex vivo on intracellular amastigotes of L. infantum (Li) and L. donovani (Ld), causative agents of human and canine visceral leishmaniasis (VL). In addition, the potential synergistic effect of the most active molecule and well-established antileishmanial drugs against promastigotes was explored. Dehydroisosilybin A elicited the highest inhibition against Ld and Li promastigotes with an approximate IC50 of 90.23 µM. This molecule showed a moderate synergism with amphotericin B (AmB) but not with SbIII or paromomycin, although it was ineffective against amastigotes. Antileishmanial activity on intracellular amastigotes of the two diastereoisomers of dehydrosilybin (10 µM) was comparable to that elicited by 0.1 µM AmB. Antiproliferative activity and safety of flavonolignans suggest the interest of exploring their potential value in combination therapy against VL.MDPIUniversidad Complutense de Madrid20182018-06-2720182018-06-27journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/12525reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/125252026-06-02T12:44:21Z
dc.title.none.fl_str_mv In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani
title In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani
spellingShingle In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani
Olías Molero, Ana Isabel
leishmaniasis
Leishmania infantum
L. donovani
silybin
dehydrosilybin
dehydroisosilybin
amphotericin
paromomycin
SbIII
Patología veterinaria
3109.07 Patología
title_short In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani
title_full In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani
title_fullStr In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani
title_full_unstemmed In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani
title_sort In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L. donovani
dc.creator.none.fl_str_mv Olías Molero, Ana Isabel
Jiménez Antón, María Dolores
Biedermann, David
Corral Caridad, María Jesús
Alunda Rodríguez, José María
author Olías Molero, Ana Isabel
author_facet Olías Molero, Ana Isabel
Jiménez Antón, María Dolores
Biedermann, David
Corral Caridad, María Jesús
Alunda Rodríguez, José María
author_role author
author2 Jiménez Antón, María Dolores
Biedermann, David
Corral Caridad, María Jesús
Alunda Rodríguez, José María
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv leishmaniasis
Leishmania infantum
L. donovani
silybin
dehydrosilybin
dehydroisosilybin
amphotericin
paromomycin
SbIII
Patología veterinaria
3109.07 Patología
topic leishmaniasis
Leishmania infantum
L. donovani
silybin
dehydrosilybin
dehydroisosilybin
amphotericin
paromomycin
SbIII
Patología veterinaria
3109.07 Patología
description Flavonolignans from the seeds of the milk thistle (Silybum marianum) have been extensively used in folk medicine for centuries. Confirmation of their properties as hepatoprotective, antioxidant and anticancer has been obtained using standardized extracts and purified flavonolignans. Information on their potential effect on Leishmania is very scarce. We have investigated the effect of silymarin, silybin and related flavonolignans on the multiplication of promastigotes in vitro and ex vivo on intracellular amastigotes of L. infantum (Li) and L. donovani (Ld), causative agents of human and canine visceral leishmaniasis (VL). In addition, the potential synergistic effect of the most active molecule and well-established antileishmanial drugs against promastigotes was explored. Dehydroisosilybin A elicited the highest inhibition against Ld and Li promastigotes with an approximate IC50 of 90.23 µM. This molecule showed a moderate synergism with amphotericin B (AmB) but not with SbIII or paromomycin, although it was ineffective against amastigotes. Antileishmanial activity on intracellular amastigotes of the two diastereoisomers of dehydrosilybin (10 µM) was comparable to that elicited by 0.1 µM AmB. Antiproliferative activity and safety of flavonolignans suggest the interest of exploring their potential value in combination therapy against VL.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-06-27
2018
2018-06-27
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/12525
url https://hdl.handle.net/20.500.14352/12525
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.228081

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