Chemical composition and biotoxicity of red algae Kappaphycus alvarezii Doty (Solieriaceae)
A phytochemical and bioactivity study was carried out on the chloroform, ethyl acetate and methanol extracts of the Kappaphycus alvarezii Doty algae (collected on the Island of Cubagua, Venezuela). The LC50 values&nb...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Ecuador |
| Institución: | Universidad Técnica de Manabi |
| Repositorio: | Revista Aquatechnica |
| Idioma: | español |
| OAI Identifier: | oai:ojs2.revistas.utm.edu.ec:article/2412 |
| Acceso en línea: | https://revistas.utm.edu.ec/index.php/aquatechnica/article/view/2412 |
| Access Level: | acceso abierto |
| Palabra clave: | Artemia salina Phytochemistry Kappaphycus alvarezii Secondary metabolites Toxicity Fitoquímica Metabolitos secundarios Toxicidad |
| Sumario: | A phytochemical and bioactivity study was carried out on the chloroform, ethyl acetate and methanol extracts of the Kappaphycus alvarezii Doty algae (collected on the Island of Cubagua, Venezuela). The LC50 values obtained in the bioassay performed with all the extracts, showed toxicity to crustacean larvae Artemia salina (LC50 <300 μg.ml-1). By means of column chromatography and preparative thin layer, the extracts were continuously fractionated with chloroform and ethyl acetate, some sub- fractions obtained by GC-MS were analyzed, and some of their constituents were identified by their fragmentation patterns and comparison of their mass spectra with the existing in the database. Finding that fraction A2 from the ethyl acetate extract showed hexadecanoic acid, dibutyl phthalate and the steroidal compound cholestane as major components; in addition to 2,6-diter-butylcyclohexa-2,5-diene-1,4-dione, 6,10,14- trimethyl-2-pentadecanone, 2- phenylacetic acid and 1- (1-propoxy) propane as minor components. While the FD fraction of the chloroform extract exhibited saturated palmitic, stearic and myristic fatty acids as abundant components, and 3-ethyl-4-methyl-1H- pyrrole-2,5-dione in smaller proportion. Octadecanoic acid was identified in the EM7 fraction from the methanolic extract through uni and two-dimensional NMR. 1- phenylbutan-1-ol was characterized by the same spectroscopic technique in the FG fraction of the chloroform extract. The different secondary metabolites identified are the first report for K. alvarezii and are possibly responsible for the in vitro bioactivity observed. It can be inferred that this red algae is a promising source of bioactive compounds. |
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