Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer

Antimicrobial peptides (AMPs) constitute part of a broad range of bioactive compounds present on diverse organisms, including frogs. Peptides, produced in the granular glands of amphibian skin, constitute a component of their innate immune response, providing protection against pathogenic microorgan...

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Autores: Cuesta, Sebastian A., Morales, Felipe, Pilaquinga, Fernanda, Morán Marcillo, Giovanna, Proaño Bolaños, Carolina, Blasco Zúñiga, Ailín, Rivera, Miryan, Meneses, Lorena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Ecuador
Institución:Universidad Regional Amazónica
Repositorio:Repositorio Universidad Regional Amazónica
OAI Identifier:oai:repositorio.ikiam.edu.ec:RD_IKIAM/461
Acceso en línea:https://doi.org/10.1007/s00726-021-02986-w
http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/461
Access Level:acceso abierto
Palabra clave:Cruzioseptins
Antimicrobial peptides
Amphibians skin secretion
Molecular cloning
Molecular docking
Cruziohyla calcarifer
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spelling Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcariferCuesta, Sebastian A.Morales, FelipePilaquinga, FernandaMorán Marcillo, GiovannaProaño Bolaños, CarolinaBlasco Zúñiga, AilínRivera, MiryanMeneses, LorenaCruzioseptinsAntimicrobial peptidesAmphibians skin secretionMolecular cloningMolecular dockingCruziohyla calcariferAntimicrobial peptides (AMPs) constitute part of a broad range of bioactive compounds present on diverse organisms, including frogs. Peptides, produced in the granular glands of amphibian skin, constitute a component of their innate immune response, providing protection against pathogenic microorganisms. In this work, two novel cruzioseptins peptides, cruzioseptin-16 and -17, extracted from the splendid leaf frog Cruziohyla calcarifer are presented. These peptides were identified using molecular cloning and tandem mass spectrometry. Later, peptides were synthetized using solid-phase peptide synthesis, and their minimal inhibitory concentration and haemolytic activity were tested. Furthermore, these two cruzioseptins plus three previously reported (CZS-1, CZS-2, CZS-3) were computationally characterized. Results show that cruzioseptins are 21–23 residues long alpha helical cationic peptides, with antimicrobial activity against E. coli, S. aureus, and C. albicans and low haemolytic effect. Docking results agree with the principal action mechanism of cationic AMPs that goes through cell membrane disruption due to electrostatic interactions between cationic residues in the cruzioseptins and negative phosphate groups in the pathogen cell membrane. An action mechanism through enzymes inhibition was also tried, but no conclusive results about this mechanism were obtained.Scopus2021-09-20T16:56:35Z2021-09-20T16:56:35Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCuesta, S.A., Reinoso, C., Morales, F. et al. Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer. Amino Acids 53, 853–868 (2021). doi.org/10.1007/s00726-021-02986-whttps://doi.org/10.1007/s00726-021-02986-whttp://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/461enProducción científica - Artículos Científicos;A-IKIAM-000341info:eu-repo/semantics/openAccessreponame:Repositorio Universidad Regional Amazónicainstname:Universidad Regional Amazónicainstacron:IKIAM2023-04-05T08:00:22Zoai:repositorio.ikiam.edu.ec:RD_IKIAM/461Institucionalhttps://repositorio.ikiam.edu.ec/Universidad públicahttps://www.ikiam.edu.ec/https://repositorio.ikiam.edu.ec/oaiEcuador...opendoar:02023-04-05T08:00:22falseInstitucionalhttps://repositorio.ikiam.edu.ec/Universidad públicahttps://www.ikiam.edu.ec/https://repositorio.ikiam.edu.ec/oai.Ecuador...opendoar:02023-04-05T08:00:22Repositorio Universidad Regional Amazónica - Universidad Regional Amazónicafalse
dc.title.none.fl_str_mv Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
title Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
spellingShingle Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
Cuesta, Sebastian A.
Cruzioseptins
Antimicrobial peptides
Amphibians skin secretion
Molecular cloning
Molecular docking
Cruziohyla calcarifer
title_short Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
title_full Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
title_fullStr Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
title_full_unstemmed Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
title_sort Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer
dc.creator.none.fl_str_mv Cuesta, Sebastian A.
Morales, Felipe
Pilaquinga, Fernanda
Morán Marcillo, Giovanna
Proaño Bolaños, Carolina
Blasco Zúñiga, Ailín
Rivera, Miryan
Meneses, Lorena
author Cuesta, Sebastian A.
author_facet Cuesta, Sebastian A.
Morales, Felipe
Pilaquinga, Fernanda
Morán Marcillo, Giovanna
Proaño Bolaños, Carolina
Blasco Zúñiga, Ailín
Rivera, Miryan
Meneses, Lorena
author_role author
author2 Morales, Felipe
Pilaquinga, Fernanda
Morán Marcillo, Giovanna
Proaño Bolaños, Carolina
Blasco Zúñiga, Ailín
Rivera, Miryan
Meneses, Lorena
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cruzioseptins
Antimicrobial peptides
Amphibians skin secretion
Molecular cloning
Molecular docking
Cruziohyla calcarifer
topic Cruzioseptins
Antimicrobial peptides
Amphibians skin secretion
Molecular cloning
Molecular docking
Cruziohyla calcarifer
description Antimicrobial peptides (AMPs) constitute part of a broad range of bioactive compounds present on diverse organisms, including frogs. Peptides, produced in the granular glands of amphibian skin, constitute a component of their innate immune response, providing protection against pathogenic microorganisms. In this work, two novel cruzioseptins peptides, cruzioseptin-16 and -17, extracted from the splendid leaf frog Cruziohyla calcarifer are presented. These peptides were identified using molecular cloning and tandem mass spectrometry. Later, peptides were synthetized using solid-phase peptide synthesis, and their minimal inhibitory concentration and haemolytic activity were tested. Furthermore, these two cruzioseptins plus three previously reported (CZS-1, CZS-2, CZS-3) were computationally characterized. Results show that cruzioseptins are 21–23 residues long alpha helical cationic peptides, with antimicrobial activity against E. coli, S. aureus, and C. albicans and low haemolytic effect. Docking results agree with the principal action mechanism of cationic AMPs that goes through cell membrane disruption due to electrostatic interactions between cationic residues in the cruzioseptins and negative phosphate groups in the pathogen cell membrane. An action mechanism through enzymes inhibition was also tried, but no conclusive results about this mechanism were obtained.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-20T16:56:35Z
2021-09-20T16:56:35Z
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Cuesta, S.A., Reinoso, C., Morales, F. et al. Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer. Amino Acids 53, 853–868 (2021). doi.org/10.1007/s00726-021-02986-w
https://doi.org/10.1007/s00726-021-02986-w
http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/461
identifier_str_mv Cuesta, S.A., Reinoso, C., Morales, F. et al. Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer. Amino Acids 53, 853–868 (2021). doi.org/10.1007/s00726-021-02986-w
url https://doi.org/10.1007/s00726-021-02986-w
http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/461
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv Producción científica - Artículos Científicos;A-IKIAM-000341
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Scopus
publisher.none.fl_str_mv Scopus
dc.source.none.fl_str_mv reponame:Repositorio Universidad Regional Amazónica
instname:Universidad Regional Amazónica
instacron:IKIAM
instname_str Universidad Regional Amazónica
instacron_str IKIAM
institution IKIAM
reponame_str Repositorio Universidad Regional Amazónica
collection Repositorio Universidad Regional Amazónica
repository.name.fl_str_mv Repositorio Universidad Regional Amazónica - Universidad Regional Amazónica
repository.mail.fl_str_mv .
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