Propagation of Dendrobium aggregatum by green capsule culture

An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP...

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Detalles Bibliográficos
Autores: Vijayakumar, S., Rajalkshmi, G., Kalimuthu, K.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Costa Rica
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Idioma:inglés
OAI Identifier:oai:portal.ucr.ac.cr:article/11763
Acceso en línea:https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763
Access Level:acceso abierto
Palabra clave:dendrobium
protocorm like bodies
acclimatization
in vitro
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spelling Propagation of Dendrobium aggregatum by green capsule culturePropagation of Dendrobium aggregatum by green capsule cultureVijayakumar, S.Rajalkshmi, G.Kalimuthu, K.dendrobiumprotocorm like bodiesacclimatizationin vitrodendrobiumprotocorm like bodiesacclimatizationin vitroAn efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.Universidad de Costa Rica2013-09-04info:eu-repo/semantics/publishedVersionArticleartículo originalhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/1176310.15517/lank.v0i0.11763Lankesteriana: International Journal on Orchidology; 2012: Lankesteriana: Volumen 12, Número 2Lankesteriana: International Journal on Orchidology; 2012: Lankesteriana: Volumen 12, Número 22215-20671409-3871reponame:Portal de Revistas UCRinstname:Universidad de Costa Ricainstacron:UCRenghttps://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763/11080acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccess2022-07-20T01:01:07Zoai:portal.ucr.ac.cr:article/11763Portal de revistashttps://revistas.ucr.ac.cr/Universidadhttp://www.ucr.ac.crhttps://revistas.ucr.ac.cr/index.php/index/oaijorge.polanco@ucr.ac.crCosta RicaNo aplicaNo aplicaNo aplicaopendoar:2025-08-13T10:50:45.147Portal de Revistas UCR - Universidad de Costa Ricafalse
dc.title.none.fl_str_mv Propagation of Dendrobium aggregatum by green capsule culture
Propagation of Dendrobium aggregatum by green capsule culture
title Propagation of Dendrobium aggregatum by green capsule culture
spellingShingle Propagation of Dendrobium aggregatum by green capsule culture
Vijayakumar, S.
dendrobium
protocorm like bodies
acclimatization
in vitro
dendrobium
protocorm like bodies
acclimatization
in vitro
title_short Propagation of Dendrobium aggregatum by green capsule culture
title_full Propagation of Dendrobium aggregatum by green capsule culture
title_fullStr Propagation of Dendrobium aggregatum by green capsule culture
title_full_unstemmed Propagation of Dendrobium aggregatum by green capsule culture
title_sort Propagation of Dendrobium aggregatum by green capsule culture
dc.creator.none.fl_str_mv Vijayakumar, S.
Rajalkshmi, G.
Kalimuthu, K.
author Vijayakumar, S.
author_facet Vijayakumar, S.
Rajalkshmi, G.
Kalimuthu, K.
author_role author
author2 Rajalkshmi, G.
Kalimuthu, K.
author2_role author
author
dc.subject.none.fl_str_mv dendrobium
protocorm like bodies
acclimatization
in vitro
dendrobium
protocorm like bodies
acclimatization
in vitro
topic dendrobium
protocorm like bodies
acclimatization
in vitro
dendrobium
protocorm like bodies
acclimatization
in vitro
description An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-04
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Article
artículo original
http://purl.org/coar/resource_type/c_2df8fbb1
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763
10.15517/lank.v0i0.11763
url https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763
identifier_str_mv 10.15517/lank.v0i0.11763
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763/11080
dc.rights.none.fl_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
rights_invalid_str_mv acceso abierto
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Lankesteriana: International Journal on Orchidology; 2012: Lankesteriana: Volumen 12, Número 2
Lankesteriana: International Journal on Orchidology; 2012: Lankesteriana: Volumen 12, Número 2
2215-2067
1409-3871
reponame:Portal de Revistas UCR
instname:Universidad de Costa Rica
instacron:UCR
instname_str Universidad de Costa Rica
instacron_str UCR
institution UCR
reponame_str Portal de Revistas UCR
collection Portal de Revistas UCR
repository.name.fl_str_mv Portal de Revistas UCR - Universidad de Costa Rica
repository.mail.fl_str_mv jorge.polanco@ucr.ac.cr
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score 15.198674

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