Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.

CARNEIRO, M. C.; CASTRO, A. C. S. e; SILVA, R. R.; CHAVES, J. E. V.; SILVA, V. A. O.; CAPOBIANCO, N. E.; QUEIROZ, P. J. B.; MELO, L. de F. e; BARBOSA, E. A.; DODE, M. A. N.; SPRÍCIGO, J. F. W.

Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.

This study investigated the motility and fertility of frozen-thawed semen from Pantaneiro bulls, comparing samples collected via ejaculation and directly from the epididymis. The aim was to evaluate the potential of epididymal semen for use in conservation programs by assessing its post-thaw motilit...

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Detalles Bibliográficos
Autores:	CARNEIRO, M. C., CASTRO, A. C. S. e, SILVA, R. R., CHAVES, J. E. V., SILVA, V. A. O., CAPOBIANCO, N. E., QUEIROZ, P. J. B., MELO, L. de F. e, BARBOSA, E. A., DODE, M. A. N., SPRÍCIGO, J. F. W.
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2025
País:	Brasil
Institución:	Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Repositorio:	Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Idioma:	inglés
OAI Identifier:	oai:www.alice.cnptia.embrapa.br:doc/1176577
Acceso en línea:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1176577 https://doi.org/10.3390/biology14050465
Access Level:	acceso abierto
Palabra clave:	Indigenous cattle Semen cryopreservation IVP Molecular marker

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spelling	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.Indigenous cattleSemen cryopreservationIVPMolecular markerThis study investigated the motility and fertility of frozen-thawed semen from Pantaneiro bulls, comparing samples collected via ejaculation and directly from the epididymis. The aim was to evaluate the potential of epididymal semen for use in conservation programs by assessing its post-thaw motility and in vitro fertility. Semen from five bulls was analyzed for fresh quality, cell-free DNA (cfDNA) levels, and sperm performance after freezing. Results showed that epididymal semen had fewer defects and exhibited superior motility after thawing compared to ejaculated semen. Despite these differences, both semen sources were equally effective in fertilizing oocytes in vitro. The characterization of cfDNA levels adds valuable insights to the field of male fertility and supports efforts to conserve indigenous cattle breeds. This research contributes to improving semen preservation techniques and highlights the motility of epididymal semen as a reproductive resource in livestock breeding and conservation strategies.MARCOS COURA CARNEIRO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ALICE CAROLINE SOUZA E CASTRO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ROBERTA REIS SILVA, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); JOSÉ EDUARDO VIEIRA CHAVES, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); VENÂNCIO AUGUSTO OLIVEIRA SILVA, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); NATALIA ERNANDES CAPOBIANCO; PAULO JOSÉ BASTOS QUEIROZ, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); LEONARDO DE FRANÇA E MELO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ELEONORA ARAÚJO BARBOSA, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; MARGOT ALVES NUNES DODE, CENARGEN; JOSÉ FELIPE WARMLING SPRÍCIGO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG).2025-06-12T17:47:38Z2025-06-12T17:47:38Z2025-06-122025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBiology, v. 14, 465, 2025.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1176577https://doi.org/10.3390/biology14050465enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPACARNEIRO, M. C.CASTRO, A. C. S. eSILVA, R. R.CHAVES, J. E. V.SILVA, V. A. O.CAPOBIANCO, N. E.QUEIROZ, P. J. B.MELO, L. de F. eBARBOSA, E. A.DODE, M. A. N.SPRÍCIGO, J. F. W.2025-06-14T11:36:47Zoai:www.alice.cnptia.embrapa.br:doc/1176577Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542025-06-14T11:36:47Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.
title	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.
spellingShingle	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls. CARNEIRO, M. C. Indigenous cattle Semen cryopreservation IVP Molecular marker
title_short	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.
title_full	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.
title_fullStr	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.
title_full_unstemmed	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.
title_sort	Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.
dc.creator.none.fl_str_mv	CARNEIRO, M. C. CASTRO, A. C. S. e SILVA, R. R. CHAVES, J. E. V. SILVA, V. A. O. CAPOBIANCO, N. E. QUEIROZ, P. J. B. MELO, L. de F. e BARBOSA, E. A. DODE, M. A. N. SPRÍCIGO, J. F. W.
author	CARNEIRO, M. C.
author_facet	CARNEIRO, M. C. CASTRO, A. C. S. e SILVA, R. R. CHAVES, J. E. V. SILVA, V. A. O. CAPOBIANCO, N. E. QUEIROZ, P. J. B. MELO, L. de F. e BARBOSA, E. A. DODE, M. A. N. SPRÍCIGO, J. F. W.
author_role	author
author2	CASTRO, A. C. S. e SILVA, R. R. CHAVES, J. E. V. SILVA, V. A. O. CAPOBIANCO, N. E. QUEIROZ, P. J. B. MELO, L. de F. e BARBOSA, E. A. DODE, M. A. N. SPRÍCIGO, J. F. W.
author2_role	author author author author author author author author author author
dc.contributor.none.fl_str_mv	MARCOS COURA CARNEIRO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ALICE CAROLINE SOUZA E CASTRO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ROBERTA REIS SILVA, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); JOSÉ EDUARDO VIEIRA CHAVES, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); VENÂNCIO AUGUSTO OLIVEIRA SILVA, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); NATALIA ERNANDES CAPOBIANCO; PAULO JOSÉ BASTOS QUEIROZ, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); LEONARDO DE FRANÇA E MELO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG); ELEONORA ARAÚJO BARBOSA, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; MARGOT ALVES NUNES DODE, CENARGEN; JOSÉ FELIPE WARMLING SPRÍCIGO, UNIVERSIDADE FEDERAL DE GOIÁS (UFG).
dc.subject.por.fl_str_mv	Indigenous cattle Semen cryopreservation IVP Molecular marker
topic	Indigenous cattle Semen cryopreservation IVP Molecular marker
description	This study investigated the motility and fertility of frozen-thawed semen from Pantaneiro bulls, comparing samples collected via ejaculation and directly from the epididymis. The aim was to evaluate the potential of epididymal semen for use in conservation programs by assessing its post-thaw motility and in vitro fertility. Semen from five bulls was analyzed for fresh quality, cell-free DNA (cfDNA) levels, and sperm performance after freezing. Results showed that epididymal semen had fewer defects and exhibited superior motility after thawing compared to ejaculated semen. Despite these differences, both semen sources were equally effective in fertilizing oocytes in vitro. The characterization of cfDNA levels adds valuable insights to the field of male fertility and supports efforts to conserve indigenous cattle breeds. This research contributes to improving semen preservation techniques and highlights the motility of epididymal semen as a reproductive resource in livestock breeding and conservation strategies.
publishDate	2025
dc.date.none.fl_str_mv	2025-06-12T17:47:38Z 2025-06-12T17:47:38Z 2025-06-12 2025
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	Biology, v. 14, 465, 2025. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1176577 https://doi.org/10.3390/biology14050465
identifier_str_mv	Biology, v. 14, 465, 2025.
url	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1176577 https://doi.org/10.3390/biology14050465
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA
instname_str	Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str	EMBRAPA
institution	EMBRAPA
reponame_str	Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection	Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv	Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv	cg-riaa@embrapa.br
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Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.

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