Comparison of post-thaw motility and in vitro fertility between ejaculated and epididymal semen, and seminal cfDNA characterization in Pantaneiro bulls.

This study investigated the motility and fertility of frozen-thawed semen from Pantaneiro bulls, comparing samples collected via ejaculation and directly from the epididymis. The aim was to evaluate the potential of epididymal semen for use in conservation programs by assessing its post-thaw motilit...

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Detalles Bibliográficos
Autores: CARNEIRO, M. C., CASTRO, A. C. S. e, SILVA, R. R., CHAVES, J. E. V., SILVA, V. A. O., CAPOBIANCO, N. E., QUEIROZ, P. J. B., MELO, L. de F. e, BARBOSA, E. A., DODE, M. A. N., SPRÍCIGO, J. F. W.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:Brasil
Institución:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
Repositorio:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Idioma:inglés
OAI Identifier:oai:www.alice.cnptia.embrapa.br:doc/1176577
Acceso en línea:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1176577
https://doi.org/10.3390/biology14050465
Access Level:acceso abierto
Palabra clave:Indigenous cattle
Semen cryopreservation
IVP
Molecular marker
Descripción
Sumario:This study investigated the motility and fertility of frozen-thawed semen from Pantaneiro bulls, comparing samples collected via ejaculation and directly from the epididymis. The aim was to evaluate the potential of epididymal semen for use in conservation programs by assessing its post-thaw motility and in vitro fertility. Semen from five bulls was analyzed for fresh quality, cell-free DNA (cfDNA) levels, and sperm performance after freezing. Results showed that epididymal semen had fewer defects and exhibited superior motility after thawing compared to ejaculated semen. Despite these differences, both semen sources were equally effective in fertilizing oocytes in vitro. The characterization of cfDNA levels adds valuable insights to the field of male fertility and supports efforts to conserve indigenous cattle breeds. This research contributes to improving semen preservation techniques and highlights the motility of epididymal semen as a reproductive resource in livestock breeding and conservation strategies.

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