Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins

Neohelice granulata were collected during a bloom dominated by Microcystis sp. in Patos Lagoon (RS, Brazil) and then sacrificed at different times of depuration in laboratory in order to verify microcystin (MC) content and toxic effects in hepatopancreas of the estuarine crab. Biochemical measuremen...

ver descrição completa

Detalhes bibliográficos
Autores: Dewes, Ligia Johansen, Monserrat, José María, Ihara, Priscilla Mieko, Yunes, João Sarkis
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2007
País:Brasil
Recursos:Universidade Federal do Rio Grande (FURG)
Repositório:Repositório Institucional da FURG (RI FURG)
Idioma:inglês
OAI Identifier:oai:repositorio.furg.br:1/1323
Acesso em linha:http://repositorio.furg.br/handle/1/1323
Access Level:Acceso aberto
Palavra-chave:Neohelice granulata
Cyanobacteria bloom
Depuration
Microcystins
Hepatopancreas
Oxidative damage
id BR_cfe82e0e458c66ae1cd3dde6559812ad
oai_identifier_str oai:repositorio.furg.br:1/1323
network_acronym_str BR
network_name_str Brasil
repository_id_str
spelling Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystinsAcúmulo de toxina e alterações bioquímicas no caranguejo estuarino Neohelice granulata (Decapoda, Bracyura) após exposição às microcistinasNeohelice granulataCyanobacteria bloomDepurationMicrocystinsHepatopancreasOxidative damageNeohelice granulata were collected during a bloom dominated by Microcystis sp. in Patos Lagoon (RS, Brazil) and then sacrificed at different times of depuration in laboratory in order to verify microcystin (MC) content and toxic effects in hepatopancreas of the estuarine crab. Biochemical measurements were: lipid peroxidation (LPO), activity of glutathione-S-transferase (GST), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). No variation of crab biochemical parameters and MC content mean value = 32 μg kg–1) was verified during the depuration period. MC content of the lyophilized bloom sample was determined by HPLC (0.129 μg mg–1). MC concentration in water at the sampling site was 1.92 μg L–1. Experimental assays were also performed via oral exposure (by gavage), in doses and time exposure varying between 0 and 55 μg kg–1 and 48 and 96 h. Analyzed variables were: GST activity (remained unaltered in all experimental conditions), LPO (augmented after 48 h in doses higher than 5.5 μg kg–1 but the opposite was observed after 96 h at the same doses) and oxygen consumption (increased in all doses and times of exposure). We conclude that (1) there is an absent or low depuration rate of MC; (2) oxidative damage should be attenuated by antioxidant defenses other than GST; (3) higher oxygen consumption should favor reactive oxygen species generation.2011-11-04T10:43:08Z2011-11-04T10:43:08Z2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfDEWES, Ligia Johansen et al. Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins. Journal Of The Brazilian Society Of Ecotoxicology, v. 2, p. 201-207, 2007. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=39>. Acesso em: 21 ago. 2011.1809-8401http://repositorio.furg.br/handle/1/1323enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGDewes, Ligia JohansenMonserrat, José MaríaIhara, Priscilla MiekoYunes, João Sarkis2023-01-05T23:39:52Zoai:repositorio.furg.br:1/1323Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestrepositorio@furg.br||sib.bdtd@furg.bropendoar:2023-01-05T23:39:52Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins
Acúmulo de toxina e alterações bioquímicas no caranguejo estuarino Neohelice granulata (Decapoda, Bracyura) após exposição às microcistinas
title Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins
spellingShingle Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins
Dewes, Ligia Johansen
Neohelice granulata
Cyanobacteria bloom
Depuration
Microcystins
Hepatopancreas
Oxidative damage
title_short Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins
title_full Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins
title_fullStr Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins
title_full_unstemmed Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins
title_sort Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins
dc.creator.none.fl_str_mv Dewes, Ligia Johansen
Monserrat, José María
Ihara, Priscilla Mieko
Yunes, João Sarkis
author Dewes, Ligia Johansen
author_facet Dewes, Ligia Johansen
Monserrat, José María
Ihara, Priscilla Mieko
Yunes, João Sarkis
author_role author
author2 Monserrat, José María
Ihara, Priscilla Mieko
Yunes, João Sarkis
author2_role author
author
author
dc.subject.por.fl_str_mv Neohelice granulata
Cyanobacteria bloom
Depuration
Microcystins
Hepatopancreas
Oxidative damage
topic Neohelice granulata
Cyanobacteria bloom
Depuration
Microcystins
Hepatopancreas
Oxidative damage
description Neohelice granulata were collected during a bloom dominated by Microcystis sp. in Patos Lagoon (RS, Brazil) and then sacrificed at different times of depuration in laboratory in order to verify microcystin (MC) content and toxic effects in hepatopancreas of the estuarine crab. Biochemical measurements were: lipid peroxidation (LPO), activity of glutathione-S-transferase (GST), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). No variation of crab biochemical parameters and MC content mean value = 32 μg kg–1) was verified during the depuration period. MC content of the lyophilized bloom sample was determined by HPLC (0.129 μg mg–1). MC concentration in water at the sampling site was 1.92 μg L–1. Experimental assays were also performed via oral exposure (by gavage), in doses and time exposure varying between 0 and 55 μg kg–1 and 48 and 96 h. Analyzed variables were: GST activity (remained unaltered in all experimental conditions), LPO (augmented after 48 h in doses higher than 5.5 μg kg–1 but the opposite was observed after 96 h at the same doses) and oxygen consumption (increased in all doses and times of exposure). We conclude that (1) there is an absent or low depuration rate of MC; (2) oxidative damage should be attenuated by antioxidant defenses other than GST; (3) higher oxygen consumption should favor reactive oxygen species generation.
publishDate 2007
dc.date.none.fl_str_mv 2007
2011-11-04T10:43:08Z
2011-11-04T10:43:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv DEWES, Ligia Johansen et al. Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins. Journal Of The Brazilian Society Of Ecotoxicology, v. 2, p. 201-207, 2007. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=39>. Acesso em: 21 ago. 2011.
1809-8401
http://repositorio.furg.br/handle/1/1323
identifier_str_mv DEWES, Ligia Johansen et al. Toxin accumulation and biochemical alterations in the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after exposure to microcystins. Journal Of The Brazilian Society Of Ecotoxicology, v. 2, p. 201-207, 2007. Disponível em: <http://ecotoxbrasil.org.br/index.php?option=com_rokdownloads&view=folder&Itemid=39>. Acesso em: 21 ago. 2011.
1809-8401
url http://repositorio.furg.br/handle/1/1323
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv repositorio@furg.br||sib.bdtd@furg.br
_version_ 1853661175027335168
score 15.301629

Registros relacionados