Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxid...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/54228 |
| Acceso en línea: | http://hdl.handle.net/11336/54228 |
| Access Level: | acceso abierto |
| Palabra clave: | Elisa Estuarine Crab Hepatopancreas Microcystin Depuration Toxin Bioaccumulation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication. |
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