In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells

Propolis is a sticky dark-colored material showing a very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activitie...

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Authors: Bufalo, Michelle C. [UNESP], Candeias, Joao M. G. [UNESP], Sforcin, Jose Mauricio [UNESP]
Format: article
Status:Published version
Publication Date:2009
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:English
OAI Identifier:oai:repositorio.unesp.br:11449/18267
Online Access:http://dx.doi.org/10.1093/ecam/nem147
http://hdl.handle.net/11449/18267
Access Level:Open access
Keyword:antitumor action
HEp-2 cells
propolis
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spelling In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cellsantitumor actionHEp-2 cellspropolisPropolis is a sticky dark-colored material showing a very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activities, among others. Its antitumor action in vivo and in vitro has also been reported, using propolis extracts or its isolated compounds. The goal of this work was to evaluate propoliss cytotoxic action in vitro on human laryngeal epidermoid carcinoma (Hep-2) cells. These cells were incubated with different concentrations of this bee product for different time periods, and morphology and the number of viable HEp-2 cells analyzed. Data showed that propolis exhibited a cytotoxic effect in vitro against HEp-2 cells, in a dose- and time-dependent way. Propolis solvent had no effects on morphology and number of viable cells, proving that the cytotoxic effects were exclusively due to propolis components. Since humans have been using propolis for a long time, further assays will provide a better comprehension of propoliss antitumor action.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP, Biosci Inst, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilUNESP, Biosci Inst, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilOxford University PressUniversidade Estadual Paulista (Unesp)2014-05-20T13:51:09Z2014-05-20T13:51:09Z2009-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article483-487application/pdfhttp://dx.doi.org/10.1093/ecam/nem147Evidence-based Complementary and Alternative Medicine. Oxford: Oxford Univ Press, v. 6, n. 4, p. 483-487, 2009.1741-427Xhttp://hdl.handle.net/11449/1826710.1093/ecam/nem147WOS:000272179600010WOS000272179600010.pdf0000-0003-1515-702XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine2.064info:eu-repo/semantics/openAccessBufalo, Michelle C. [UNESP]Candeias, Joao M. G. [UNESP]Sforcin, Jose Mauricio [UNESP]2024-10-14T15:06:11Zoai:repositorio.unesp.br:11449/18267Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-10-14T15:06:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells
title In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells
spellingShingle In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells
Bufalo, Michelle C. [UNESP]
antitumor action
HEp-2 cells
propolis
title_short In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells
title_full In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells
title_fullStr In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells
title_full_unstemmed In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells
title_sort In vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells
dc.creator.none.fl_str_mv Bufalo, Michelle C. [UNESP]
Candeias, Joao M. G. [UNESP]
Sforcin, Jose Mauricio [UNESP]
author Bufalo, Michelle C. [UNESP]
author_facet Bufalo, Michelle C. [UNESP]
Candeias, Joao M. G. [UNESP]
Sforcin, Jose Mauricio [UNESP]
author_role author
author2 Candeias, Joao M. G. [UNESP]
Sforcin, Jose Mauricio [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.subject.por.fl_str_mv antitumor action
HEp-2 cells
propolis
topic antitumor action
HEp-2 cells
propolis
description Propolis is a sticky dark-colored material showing a very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activities, among others. Its antitumor action in vivo and in vitro has also been reported, using propolis extracts or its isolated compounds. The goal of this work was to evaluate propoliss cytotoxic action in vitro on human laryngeal epidermoid carcinoma (Hep-2) cells. These cells were incubated with different concentrations of this bee product for different time periods, and morphology and the number of viable HEp-2 cells analyzed. Data showed that propolis exhibited a cytotoxic effect in vitro against HEp-2 cells, in a dose- and time-dependent way. Propolis solvent had no effects on morphology and number of viable cells, proving that the cytotoxic effects were exclusively due to propolis components. Since humans have been using propolis for a long time, further assays will provide a better comprehension of propoliss antitumor action.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
2014-05-20T13:51:09Z
2014-05-20T13:51:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/ecam/nem147
Evidence-based Complementary and Alternative Medicine. Oxford: Oxford Univ Press, v. 6, n. 4, p. 483-487, 2009.
1741-427X
http://hdl.handle.net/11449/18267
10.1093/ecam/nem147
WOS:000272179600010
WOS000272179600010.pdf
0000-0003-1515-702X
url http://dx.doi.org/10.1093/ecam/nem147
http://hdl.handle.net/11449/18267
identifier_str_mv Evidence-based Complementary and Alternative Medicine. Oxford: Oxford Univ Press, v. 6, n. 4, p. 483-487, 2009.
1741-427X
10.1093/ecam/nem147
WOS:000272179600010
WOS000272179600010.pdf
0000-0003-1515-702X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Evidence-based Complementary and Alternative Medicine
2.064
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 483-487
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1853671976011300864
score 15,223283

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