Free radical scavenging activity of Pterogyne nitens Tul. (Fabaceae)

As part of our ongoing research on antioxidant agents from Brazilian flora, twenty extracts and fractions obtained from Pterogyne nitens Tulasne (Fabaceae) were screened for free radical scavenging activity by using ABTS [2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)] and DPPH (2,2di...

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Detalhes bibliográficos
Autores: Regasini, Luis Octavio [UNESP], de Oliveira, Camila Martins [UNESP], Rebuglio Vellosa, Jose Carlos [UNESP], Mascarenhas de Faria Oliveira, Olga Maria [UNESP], Siqueira Silva, Dulce Helena [UNESP], Bolzani, Vanderlan da Silva [UNESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/25262
Acesso em linha:http://dx.doi.org/10.5897/AJB08.883
http://hdl.handle.net/11449/25262
Access Level:acceso abierto
Palavra-chave:Antioxidant
free radical scavenging activity
DPPH
ABTS
Pterogyne nitens
Leguminosae
Fabaceae
Caesalpinioideae
flavonoid
flavonol
Descrição
Resumo:As part of our ongoing research on antioxidant agents from Brazilian flora, twenty extracts and fractions obtained from Pterogyne nitens Tulasne (Fabaceae) were screened for free radical scavenging activity by using ABTS [2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)] and DPPH (2,2diphenyl- 1-picrylhydrazyl-hydrate) radicals colorimetric assay and beta-carotene bleaching test. The strongest activity was found in ethyl acetate fraction from the stem barks, exhibiting IC(50) values (in mu g/ml) of 2.10 +/- 0.1 and 10.2 +/- 0.3 on ABTS(center dot+) and DPPH(center dot), respectively. Additionally, chromatographic fractionation of stem barks yielding myricetin, quercitrin and mirycetrin, three flavonols with remarkable antioxidant activity.

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