Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis

Background: This study evaluated the effect of the solvent on the extraction of antioxidant compounds from black mulberry (Morus nigra), blackberry (Rubus ulmifolius) and strawberry (Fragaria x ananassa). Different extracts of each berry were evaluated from the determination of total phenolic conten...

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Detalhes bibliográficos
Autores: Boeing, Joana Schuelter, Barizao, Erica Oliveira, Costa e Silva, Beatriz [UNESP], Montanher, Paula Fernandes, Almeida, Vitor de Cinque, Visentainer, Jesui Vergilio
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2014
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositório:Repositório Institucional da UNESP
Idioma:inglês
OAI Identifier:oai:repositorio.unesp.br:11449/117338
Acesso em linha:http://dx.doi.org/10.1186/s13065-014-0048-1
http://hdl.handle.net/11449/117338
Access Level:Acceso aberto
Palavra-chave:Solvent extraction
Morus nigra
Rubus ulmifolius
Fragaria x ananassa
Anthocyanins
Multivariate analysis
Descrição
Resumo:Background: This study evaluated the effect of the solvent on the extraction of antioxidant compounds from black mulberry (Morus nigra), blackberry (Rubus ulmifolius) and strawberry (Fragaria x ananassa). Different extracts of each berry were evaluated from the determination of total phenolic content, anthocyanin content and antioxidant capacity, and data were applied to the principal component analysis (PCA) to gain an overview of the effect of the solvent in extraction method.Results: For all the berries analyzed, acetone/water (70/30, v/v) solvent mixture was more efficient solvent in the extracting of phenolic compounds, and methanol/water/acetic acid (70/29.5/0.5, v/v/v) showed the best values for anthocyanin content. Mixtures of ethanol/water (50/50, v/v), acetone water/acetic acid (70/29.5/0.5, v/v/v) and acetone/water (50/50, v/v) presented the highest antioxidant capacities for black mulberries, blackberries and strawberries, respectively.Conclusion: Antioxidants extractions are extremely affected by the solvent combination used. In addition, the obtained extracts with the organic solvent-water mixtures were distinguished from the extracts obtained with pure organic solvents, through the PCA analysis.