A cross-sectional study on canine Leishmania (L) infantum chagasi infection in Amazonian Brazil ratifies a higher prevalence of specific IgG-antibody response than delayed-type hypersensitivity in symptomatic and asymptomatic dogs
This was a cross-sectional study which analyzed the prevalence and the clinical and immunological spectrum of canine Leishmania (L.) infantum chagasi infection in a cohort of 320 mongrel dogs living in an endemic area of American visceral leishmaniasis in the Amazonian Brazil by using, mainly, the i...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Brasil |
| Institución: | Instituto Evandro Chagas (IEC) |
| Repositorio: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
| Idioma: | inglés |
| OAI Identifier: | oai:patua.iec.gov.br:iec/868 |
| Acceso en línea: | https://patua.iec.gov.br/handle/iec/868 |
| Access Level: | acceso abierto |
| Palabra clave: | Leishmania infantum / parasitologia Leishmaniose Visceral / transmissão Estudos Transversais Saúde Pública |
| Sumario: | This was a cross-sectional study which analyzed the prevalence and the clinical and immunological spectrum of canine Leishmania (L.) infantum chagasi infection in a cohort of 320 mongrel dogs living in an endemic area of American visceral leishmaniasis in the Amazonian Brazil by using, mainly, the indirect fluorescence antibody test (IFAT-IgG) and the delayed-type hypersensitivity (DTH), and the parasite research by the popliteal lymph node aspiration. The IFAT and DTH reactivity recognized three different immune response profiles: (1) IFAT((+))/DTH((-)) (107 dogs), (2) IFAT((-))/DTH((+)) (18 dogs), and (3) IFAT((+))/DTH((+)) (13 dogs), providing an overall prevalence of infection of 43 per cent (138/320). Thus, the specific prevalence of IFAT( (+) )/DTH( (-) ) 33.4 per cent (107/320) was higher than those of IFAT( (-) )/DTH( (+) ) 5.6 per cent (18/320) and IFAT( (+) )/DTH( (+) ) 4.0 per cent (13/320). Moreover, the frequency of these profiles among 138 infected dogs showed that the IFAT( (+) )/DTH( (-) ) rate of 77.5 per cent (107/138) was also higher than those of 13.0 per cent (18/138) of IFAT( (-) )/DTH( (+) ) and 9.5 per cent (13/138) of IFAT( (+) )/DTH( (+) ) rates. The frequency of asymptomatic dogs (76 per cent-105) was higher than those of symptomatic (16.6 per cent-23) and oligosymptomatic ones (7.4 per cent-10). A total of 16 (11.6 per cent) L. (L.) i. chagasi isolates were obtained from infected dogs, all from the IFAT( (+) ) /DTH( (-) ) profile: 41 per cent (9/22) from symptomatic, 33.3 per cent (3/9) from oligosymptomatic, and 5.2 per cent (4/76) from asymptomatic dogs. These findings strongly suggested that despite the higher frequency of asymptomatic dogs (76 per cent-105), the majority (72.4 per cent-76) was characterized by the IFAT( (+) ) /DTH( (-) ) profile with a doubtful immunogenetic resistance against infection. |
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