Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571

The objective of this study was to investigate the process of immobilization of inulinases using natural montmorillonite as inorganic support. The enzyme to buffer ratio of 3:10 and 10 min of immobilization led to the highest specific activity, 375.07 U/mg protein. The immobilized inulinase kept its...

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Autores: Coghetto, Chaline C., Scherer, Robison P., Silva, Marceli F., Golunski, Simone, Pergher, Sibele Berenice Castellã, Oliveira, Débora de, Oliveira, J. Vladimir, Treichel, Helen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Brasil
Institución:Universidade Federal do Rio Grande do Norte (UFRN)
Repositorio:Repositório Institucional da UFRN
Idioma:inglés
OAI Identifier:oai:repositorio.ufrn.br:123456789/29187
Acceso en línea:https://repositorio.ufrn.br/jspui/handle/123456789/29187
Access Level:acceso abierto
Palabra clave:Kluyveromyces marxianus NRRL Y-7571
Inulinase
Inorganic support
Immobilization
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spelling Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571Kluyveromyces marxianus NRRL Y-7571InulinaseInorganic supportImmobilizationThe objective of this study was to investigate the process of immobilization of inulinases using natural montmorillonite as inorganic support. The enzyme to buffer ratio of 3:10 and 10 min of immobilization led to the highest specific activity, 375.07 U/mg protein. The immobilized inulinase kept its activity after 1968 h under storage at low temperatures and after 456–1826 h at high temperatures. The pH value of 3.5 led to the highest specific activity. Km values of 1.46 and 0.38 mM, and vmax of 0.2487 and 0.2396 mol/L min, were obtained, respectively, for sucrose and inulin.Elsevier2020-06-09T01:47:09Z2020-06-09T01:47:09Z2012-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCOGHETTO, Chaline C.; SCHERER, Robison P.; SILVA, Marceli F.; GOLUNSKI, Simone; PERGHER, Sibele Berenice Castellã; OLIVEIRA, Débora de; OLIVEIRA, J. Vladimir; TREICHEL, Helen. Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 284-289, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.005. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001028. Acesso em: 08 jun. 2020.1878-8181https://repositorio.ufrn.br/jspui/handle/123456789/2918710.1016/j.bcab.2012.06.005ark:/41046/001300000qnjsengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCoghetto, Chaline C.Scherer, Robison P.Silva, Marceli F.Golunski, SimonePergher, Sibele Berenice CastellãOliveira, Débora deOliveira, J. VladimirTreichel, Helen2023-01-26T17:54:54Zoai:repositorio.ufrn.br:123456789/29187Repositório InstitucionalPUBhttp://repositorio.ufrn.br/oai/repositorio@bczm.ufrn.bropendoar:2023-01-26T17:54:54Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.none.fl_str_mv Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
title Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
spellingShingle Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
Coghetto, Chaline C.
Kluyveromyces marxianus NRRL Y-7571
Inulinase
Inorganic support
Immobilization
title_short Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
title_full Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
title_fullStr Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
title_full_unstemmed Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
title_sort Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
dc.creator.none.fl_str_mv Coghetto, Chaline C.
Scherer, Robison P.
Silva, Marceli F.
Golunski, Simone
Pergher, Sibele Berenice Castellã
Oliveira, Débora de
Oliveira, J. Vladimir
Treichel, Helen
author Coghetto, Chaline C.
author_facet Coghetto, Chaline C.
Scherer, Robison P.
Silva, Marceli F.
Golunski, Simone
Pergher, Sibele Berenice Castellã
Oliveira, Débora de
Oliveira, J. Vladimir
Treichel, Helen
author_role author
author2 Scherer, Robison P.
Silva, Marceli F.
Golunski, Simone
Pergher, Sibele Berenice Castellã
Oliveira, Débora de
Oliveira, J. Vladimir
Treichel, Helen
author2_role author
author
author
author
author
author
author
dc.subject.por.fl_str_mv Kluyveromyces marxianus NRRL Y-7571
Inulinase
Inorganic support
Immobilization
topic Kluyveromyces marxianus NRRL Y-7571
Inulinase
Inorganic support
Immobilization
description The objective of this study was to investigate the process of immobilization of inulinases using natural montmorillonite as inorganic support. The enzyme to buffer ratio of 3:10 and 10 min of immobilization led to the highest specific activity, 375.07 U/mg protein. The immobilized inulinase kept its activity after 1968 h under storage at low temperatures and after 456–1826 h at high temperatures. The pH value of 3.5 led to the highest specific activity. Km values of 1.46 and 0.38 mM, and vmax of 0.2487 and 0.2396 mol/L min, were obtained, respectively, for sucrose and inulin.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
2020-06-09T01:47:09Z
2020-06-09T01:47:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv COGHETTO, Chaline C.; SCHERER, Robison P.; SILVA, Marceli F.; GOLUNSKI, Simone; PERGHER, Sibele Berenice Castellã; OLIVEIRA, Débora de; OLIVEIRA, J. Vladimir; TREICHEL, Helen. Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 284-289, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.005. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001028. Acesso em: 08 jun. 2020.
1878-8181
https://repositorio.ufrn.br/jspui/handle/123456789/29187
10.1016/j.bcab.2012.06.005
dc.identifier.dark.fl_str_mv ark:/41046/001300000qnjs
identifier_str_mv COGHETTO, Chaline C.; SCHERER, Robison P.; SILVA, Marceli F.; GOLUNSKI, Simone; PERGHER, Sibele Berenice Castellã; OLIVEIRA, Débora de; OLIVEIRA, J. Vladimir; TREICHEL, Helen. Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 284-289, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.005. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001028. Acesso em: 08 jun. 2020.
1878-8181
10.1016/j.bcab.2012.06.005
ark:/41046/001300000qnjs
url https://repositorio.ufrn.br/jspui/handle/123456789/29187
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
repository.mail.fl_str_mv repositorio@bczm.ufrn.br
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score 15,198674

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