Zerumbone from Zingiber zerumbet (L.) smith: A potential prophylactic and therapeutic agent against the cariogenic bacterium Streptococcus mutans
Background: Essential oil obtained from rhizomes of the Zingiber zerumbet (L.) Smith (popularly known in Brazil as bitter ginger) is mainly constituted by the biomolecule zerumbone, which exhibit untapped antimicrobial potential. The aim of this study was to investigate the antimicrobial activity of...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | Brasil |
| Institución: | Instituto Nacional de Pesquisas da Amazônia (INPA) |
| Repositorio: | Repositório Institucional do INPA |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio:1/15596 |
| Acceso en línea: | https://repositorio.inpa.gov.br/handle/1/15596 |
| Access Level: | acceso abierto |
| Palabra clave: | Essential Oil Plant Extract Unclassified Drug Zerumbone Zingiber Zerumbet Extract Antiinfective Agent Sesquiterpenes Animals Cell Anti-microbial Activity Bacterial Strain Biological Activity Concentration Response Controlled Study Crystallization Cytotoxicity Dental Caries Dental Prevention Dilution Drug Determination Drug Isolation Drug Purification Drug Purity High Performance Liquid Chromatography Hydrodistillation Minimum Bactericidal Concentration Minimum Inhibitory Concentration Mtt Assay Nonhuman Rhizome Streptococcus Mutans Zingiber Zerumbet Chemistry Drug Effect Isolation And Purification Microbial Sensitivity Test Microbial Viability Zingiberaceae Anti-bacterial Agents Microbial Sensitivity Tests Oils, Volatile |
| Sumario: | Background: Essential oil obtained from rhizomes of the Zingiber zerumbet (L.) Smith (popularly known in Brazil as bitter ginger) is mainly constituted by the biomolecule zerumbone, which exhibit untapped antimicrobial potential. The aim of this study was to investigate the antimicrobial activity of the zerumbone from bitter ginger rhizomes against the cariogenic agent Streptococcus mutans. Methods: Firstly, the essential oil from rhizomes of Zingiber zerumbet (L.) Smith extracted by hydrodistillation was submitted to purification and recrystallization process to obtain the zerumbone compound. The purity of zerumbone was determined through high-performance liquid chromatography analysis. Different concentrations of zerumbone were tested against the standard strain S. mutans (ATCC 35668) by using microdilution method. The speed of cidal activity was determined through a time kill-curve assay. The biological cytotoxicity activity of zerumbone was assessed using Vero cell line through MTT assay. Results: The zerumbone showed a minimum inhibitory concentration (MIC) of 250 μg/mL and a minimum bactericidal concentration (MBC) of 500 μg/mL against S. mutans. After six hours of bacteria-zerumbone interaction, all concentrations tested starts to kill the bacteria and all bacteria were killed between 48 and 72 h period at the concentration of 500 μg/mL (99,99% of bacteria were killed in comparison with original inoculum). In addition, zerumbone showed no cytotoxicity activity on mammalian continuous cells line. Conclusions: These results draw attention to the potential of zerumbone as antimicrobial agent against S. mutans infection, indicating its possible use in the phyto-pharmaceutical formulations as new approach to prevent and treat tooth decay disease. © 2018 The Author(s). |
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