Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation
Ethnopharmacological relevance: Costus spiralis (Jacq.). Roscoe (Costaceae) is traditionally used in Brazil for the treatment of kidney diseases such as pyelonephritis, urethra inflammation, kidney stones, and inflammatory conditions. There are reports of its use by Brazilian Indians since the 17th...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Brasil |
| Institución: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/79378 |
| Acceso en línea: | https://doi.org/10.1016/j.jep.2022.115510 http://hdl.handle.net/1843/79378 https://orcid.org/0000-0001-9888-3064 https://orcid.org/0000-0001-6779-4130 https://orcid.org/0000-0002-1049-6754 https://orcid.org/0000-0001-9222-3882 https://orcid.org/0000-0003-4882-4992 |
| Access Level: | acceso abierto |
| Palabra clave: | Cisplatin Costus spiralis Flavonoids Vicenin II Schaftoside Nephrotoxicity Testes de toxicidade Flavonóides Nefrologia Plantas medicinais |
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Brasil |
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| dc.title.none.fl_str_mv |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation |
| title |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation |
| spellingShingle |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation Juliana Mendes Amorim Cisplatin Costus spiralis Flavonoids Vicenin II Schaftoside Nephrotoxicity Testes de toxicidade Flavonóides Nefrologia Plantas medicinais |
| title_short |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation |
| title_full |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation |
| title_fullStr |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation |
| title_full_unstemmed |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation |
| title_sort |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigation |
| dc.creator.none.fl_str_mv |
Juliana Mendes Amorim Larissa Camila Ribeiro de Souza Carlos Alberto Tagliati Ana Cristina Simões e Silva Rachel Oliveira Castilho |
| author |
Juliana Mendes Amorim |
| author_facet |
Juliana Mendes Amorim Larissa Camila Ribeiro de Souza Carlos Alberto Tagliati Ana Cristina Simões e Silva Rachel Oliveira Castilho |
| author_role |
author |
| author2 |
Larissa Camila Ribeiro de Souza Carlos Alberto Tagliati Ana Cristina Simões e Silva Rachel Oliveira Castilho |
| author2_role |
author author author author |
| dc.subject.por.fl_str_mv |
Cisplatin Costus spiralis Flavonoids Vicenin II Schaftoside Nephrotoxicity Testes de toxicidade Flavonóides Nefrologia Plantas medicinais |
| topic |
Cisplatin Costus spiralis Flavonoids Vicenin II Schaftoside Nephrotoxicity Testes de toxicidade Flavonóides Nefrologia Plantas medicinais |
| description |
Ethnopharmacological relevance: Costus spiralis (Jacq.). Roscoe (Costaceae) is traditionally used in Brazil for the treatment of kidney diseases such as pyelonephritis, urethra inflammation, kidney stones, and inflammatory conditions. There are reports of its use by Brazilian Indians since the 17th century when it was known as “pacocatinga.” Currently, the use of the Costus species in Brazil is widespread, which was evidenced by the inclusion of the genus in the Brazilian National List of Medicinal Plants of Interest to the Unified Health System (RENISUS). Aim of the study: This study aimed to confirm the ethnopharmacological use of Costus spiralis in the treatment of kidney diseases, toxicity study using animal models, and the phytochemistry of the species. Materials and methods: The chemical profile of Costus spiralis leaves extract (CSLE) was obtained for the hydroethanolic extract by ultra-performance liquid chromatography coupled to a mass spectrometer and ultraviolet detector with diode array (UPLC-UV/DAD-ESI-MS). The acute oral toxicity of the extract was predicted using the neutral red uptake cytotoxicity assay. Wistar rats were used in a model in vivo for confirmation of acute oral toxicity (2000 mg/kg p.o. for 14 days.) and determination of the effect on a cisplatin-induced nephrotoxicity model. Results: The analysis by UPLC-UV/DAD-ESI-MS showed that the chemical composition of the extract is mostly di-glycosylated flavones of apigenin. In the extract were identified the flavones vicenin II and schaftoside. The quantification of total flavonoids by spectrometry showed 0.880%. CSLE proved to be safe for acute oral administration (2000 mg/kg) with an IC50 value of 222.9 μg/mL and predicted oral toxic dose of 523.82 μg/mL in a neutral red uptake cytotoxicity assay. The absence of death allows the classification of the extract in class 5 according to OECD 423 guidelines and therefore it can be considered as a high acute safety product, which is highly relevant, considering the wide popular use of the species. In the cisplatin-induced nephrotoxicity model, C. spiralis extract (5, 15, and 30 mg/kg) significantly improved renal function, reversing almost completely the effects on plasma creatinine levels and creatinine clearance (p < 0.001). Conclusions: This study demonstrates that oral administration of Costus spiralis extract leaves is safe and effective in restoring the renal function in rats in a cisplatin-induced nephrotoxicity. It is suggested that the observed activity is related to the flavonoids present. This hypothesis should be confirmed, and the participation of other secondary metabolites should be investigated in the future. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-12-05 2025-01-21T20:32:21Z 2025-01-21T20:32:21Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.jep.2022.115510 0378-8741 http://hdl.handle.net/1843/79378 https://orcid.org/0000-0001-9888-3064 https://orcid.org/0000-0001-6779-4130 https://orcid.org/0000-0002-1049-6754 https://orcid.org/0000-0001-9222-3882 https://orcid.org/0000-0003-4882-4992 |
| url |
https://doi.org/10.1016/j.jep.2022.115510 http://hdl.handle.net/1843/79378 https://orcid.org/0000-0001-9888-3064 https://orcid.org/0000-0001-6779-4130 https://orcid.org/0000-0002-1049-6754 https://orcid.org/0000-0001-9222-3882 https://orcid.org/0000-0003-4882-4992 |
| identifier_str_mv |
0378-8741 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais Brasil FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS MED - DEPARTAMENTO DE PEDIATRIA UFMG |
| publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais Brasil FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS MED - DEPARTAMENTO DE PEDIATRIA UFMG |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
| instacron_str |
UFMG |
| institution |
UFMG |
| reponame_str |
Repositório Institucional da UFMG |
| collection |
Repositório Institucional da UFMG |
| repository.name.fl_str_mv |
Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
| repository.mail.fl_str_mv |
repositorio@ufmg.br |
| _version_ |
1853663077916999680 |
| spelling |
Costus spiralis extract restores kidney function in cisplatin-induced nephrotoxicity model: ethnopharmacological use, chemical and toxicological investigationCisplatinCostus spiralisFlavonoidsVicenin IISchaftosideNephrotoxicityTestes de toxicidadeFlavonóidesNefrologiaPlantas medicinaisEthnopharmacological relevance: Costus spiralis (Jacq.). Roscoe (Costaceae) is traditionally used in Brazil for the treatment of kidney diseases such as pyelonephritis, urethra inflammation, kidney stones, and inflammatory conditions. There are reports of its use by Brazilian Indians since the 17th century when it was known as “pacocatinga.” Currently, the use of the Costus species in Brazil is widespread, which was evidenced by the inclusion of the genus in the Brazilian National List of Medicinal Plants of Interest to the Unified Health System (RENISUS). Aim of the study: This study aimed to confirm the ethnopharmacological use of Costus spiralis in the treatment of kidney diseases, toxicity study using animal models, and the phytochemistry of the species. Materials and methods: The chemical profile of Costus spiralis leaves extract (CSLE) was obtained for the hydroethanolic extract by ultra-performance liquid chromatography coupled to a mass spectrometer and ultraviolet detector with diode array (UPLC-UV/DAD-ESI-MS). The acute oral toxicity of the extract was predicted using the neutral red uptake cytotoxicity assay. Wistar rats were used in a model in vivo for confirmation of acute oral toxicity (2000 mg/kg p.o. for 14 days.) and determination of the effect on a cisplatin-induced nephrotoxicity model. Results: The analysis by UPLC-UV/DAD-ESI-MS showed that the chemical composition of the extract is mostly di-glycosylated flavones of apigenin. In the extract were identified the flavones vicenin II and schaftoside. The quantification of total flavonoids by spectrometry showed 0.880%. CSLE proved to be safe for acute oral administration (2000 mg/kg) with an IC50 value of 222.9 μg/mL and predicted oral toxic dose of 523.82 μg/mL in a neutral red uptake cytotoxicity assay. The absence of death allows the classification of the extract in class 5 according to OECD 423 guidelines and therefore it can be considered as a high acute safety product, which is highly relevant, considering the wide popular use of the species. In the cisplatin-induced nephrotoxicity model, C. spiralis extract (5, 15, and 30 mg/kg) significantly improved renal function, reversing almost completely the effects on plasma creatinine levels and creatinine clearance (p < 0.001). Conclusions: This study demonstrates that oral administration of Costus spiralis extract leaves is safe and effective in restoring the renal function in rats in a cisplatin-induced nephrotoxicity. It is suggested that the observed activity is related to the flavonoids present. This hypothesis should be confirmed, and the participation of other secondary metabolites should be investigated in the future.Universidade Federal de Minas GeraisBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSMED - DEPARTAMENTO DE PEDIATRIAUFMG2025-01-21T20:32:21Z2025-01-21T20:32:21Z2022-12-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdfhttps://doi.org/10.1016/j.jep.2022.1155100378-8741http://hdl.handle.net/1843/79378https://orcid.org/0000-0001-9888-3064https://orcid.org/0000-0001-6779-4130https://orcid.org/0000-0002-1049-6754https://orcid.org/0000-0001-9222-3882https://orcid.org/0000-0003-4882-4992engJournal of Ethnopharmacologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGJuliana Mendes AmorimLarissa Camila Ribeiro de SouzaCarlos Alberto TagliatiAna Cristina Simões e SilvaRachel Oliveira Castilho2025-01-21T20:32:21Zoai:repositorio.ufmg.br:1843/79378Repositório InstitucionalPUBhttps://repositorio.ufmg.br/oairepositorio@ufmg.bropendoar:2025-01-21T20:32:21Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
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15.301629 |