Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056

A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain...

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Detalhes bibliográficos
Autores: Rodrigues, José Ariévilo Gurgel, Vanderlei, Edfranck de Sousa Oliveira, Quinderé, Ana Luíza Gomes, Fontes, Bruno Pedrosa, Queiroz, Ismael Nilo Lino de, Benevides, Norma Maria Barros
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2011
País:Brasil
Recursos:Universidade Estadual de Maringá (UEM)
Repositório:Acta Scientiarum Biological Sciences
Idioma:português
OAI Identifier:oai:periodicos.uem.br/ojs:article/6056
Acesso em linha:http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056
Access Level:Acceso aberto
Palavra-chave:marine teleosts
sulfated glycosaminoglycans
blood coagulation
APTT test
teleósteos marinhos
glicosaminoglicanos sulfatados
coagulação sanguínea
teste do TTPA
Química de macromoléculas
Descrição
Resumo:A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1.

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