Proteasas de Bromeliaceae: V. Separación y purificación de sulfhidril - proteasas presentes en frutos de Bromelia balaasae Mez
Almost ripe fruits of Bromelia balansae Mez (Bromeliaceae) were blade homogeneized within cold (-20 O C ) acetone, providing a crude preparation that could be partialiy solubllized in phosphate buffer (pH 6,4) containing EDTA and cysteine 5 mM. Solutions hydrolize casein (maximum a c t ~ t ypH 6.25-...
| Autores: | , , , |
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| Tipo de documento: | artigo |
| Estado: | Versión enviada para evaluación y publicación |
| Data de publicação: | 1988 |
| País: | Argentina |
| Recursos: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repositório: | CIC Digital (CICBA) |
| Idioma: | espanhol |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/3687 |
| Acesso em linha: | https://digital.cic.gba.gob.ar/handle/11746/3687 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Farmacología y Farmacia Enzimas Hidrólisis Solubilidad |
| Resumo: | Almost ripe fruits of Bromelia balansae Mez (Bromeliaceae) were blade homogeneized within cold (-20 O C ) acetone, providing a crude preparation that could be partialiy solubllized in phosphate buffer (pH 6,4) containing EDTA and cysteine 5 mM. Solutions hydrolize casein (maximum a c t ~ t ypH 6.25-8.5O), the activity being enhanced by addition of cysteine. The crude enzyme was purified by molecular sieve chromatography (Sephadex G-75, Superfme) and ion exchange chromatography (DEAE- and CM-Sepharose). Three proteolitically active fractions were thus obtained |
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