Proteasas de Bromeliaceae: V. Separación y purificación de sulfhidril - proteasas presentes en frutos de Bromelia balaasae Mez
Almost mature fruits of Bromelia serra Griseb., blade homogenized (Waring blender) with cold acetone (-20ºC), provide a light, proteolitically active acetone powder (1, 93g%, w/w). Extractives obtained by gentle stirring with phosphate buffer pH 6,4 containing 5 mM cysteine and 5 mM EDTA (protein co...
| Autores: | , , , |
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| Tipo de documento: | artigo |
| Estado: | Versión enviada para evaluación y publicación |
| Data de publicação: | 1988 |
| País: | Argentina |
| Recursos: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repositório: | CIC Digital (CICBA) |
| Idioma: | espanhol |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/3694 |
| Acesso em linha: | https://digital.cic.gba.gob.ar/handle/11746/3694 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Farmacología y Farmacia Hidrolasas de péptidos Enzimas Cromatografía |
| Resumo: | Almost mature fruits of Bromelia serra Griseb., blade homogenized (Waring blender) with cold acetone (-20ºC), provide a light, proteolitically active acetone powder (1, 93g%, w/w). Extractives obtained by gentle stirring with phosphate buffer pH 6,4 containing 5 mM cysteine and 5 mM EDTA (protein content 8, 24 g per 100 g of powder) show good thermal stability at moderates temperatures and highest caseinolytic activity at pH 6.0-7.8. Gel- filtration (Sephadex G-75, Superfine) allows to separate one active fraction which is resolved in two new, more pursed fractions through ion-exchange chromatography (DEAE Sephacel and CM Sepharose). |
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