Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri

Toxic honeydew produced by Flatidae Epormenis cestri in Uruguay has been shown to cause among honeybees (Apis mellifera) colonies a massive larva death called “River disease”, but the intrinsic mechanisms are still unknown. Because fat body cells, oenocytes and trophocytes, are known to regulated la...

ver descrição completa

Detalhes bibliográficos
Autores: Viotti Cresci, María Helen, Juri, Pablo, Presentado, María Laura, Verdes García, José Manuel, Nogueira, Enrique, Invernizzi, Ciro, Pedrana, Graciela
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Uruguay
Recursos:Universidad de la República
Repositorio:COLIBRI
Idioma:inglés
OAI Identifier:oai:colibri.udelar.edu.uy:20.500.12008/48404
Acesso em linha:https://hdl.handle.net/20.500.12008/48404
Access Level:acceso abierto
Palavra-chave:ABEJAS
APIS MELLIFERA
APICULTURA
ENFERMEDADES
INTOXICACION
id UY_aa92b48a0636f068fb3df2e0e7eb204c
oai_identifier_str oai:colibri.udelar.edu.uy:20.500.12008/48404
network_acronym_str UY
network_name_str Uruguay
repository_id_str
dc.title.none.fl_str_mv Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
title Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
spellingShingle Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
Viotti Cresci, María Helen
ABEJAS
APIS MELLIFERA
APICULTURA
ENFERMEDADES
INTOXICACION
title_short Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
title_full Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
title_fullStr Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
title_full_unstemmed Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
title_sort Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri
dc.creator.none.fl_str_mv Viotti Cresci, María Helen
Juri, Pablo
Presentado, María Laura
Verdes García, José Manuel
Nogueira, Enrique
Invernizzi, Ciro
Pedrana, Graciela
author Viotti Cresci, María Helen
author_facet Viotti Cresci, María Helen
Juri, Pablo
Presentado, María Laura
Verdes García, José Manuel
Nogueira, Enrique
Invernizzi, Ciro
Pedrana, Graciela
author_role author
author2 Juri, Pablo
Presentado, María Laura
Verdes García, José Manuel
Nogueira, Enrique
Invernizzi, Ciro
Pedrana, Graciela
author2_role author
author
author
author
author
author
dc.contributor.filiacion.none.fl_str_mv Viotti Cresci María Helen, Universidad de la República (Uruguay). Facultad de Veterinaria
Juri Pablo, Universidad de la República (Uruguay). Facultad de Veterinaria
Presentado María Laura, Universidad de la República (Uruguay). Facultad de Veterinaria
Verdes García José Manuel, Universidad de la República (Uruguay). Facultad de Veterinaria
Nogueira Enrique, Universidad de la República (Uruguay). Facultad de Veterinaria
Invernizzi Ciro, Universidad de la República (Uruguay). Facultad de Veterinaria
Pedrana Graciela, Universidad de la República (Uruguay). Facultad de Veterinaria
dc.subject.other.es.fl_str_mv ABEJAS
APIS MELLIFERA
APICULTURA
ENFERMEDADES
INTOXICACION
topic ABEJAS
APIS MELLIFERA
APICULTURA
ENFERMEDADES
INTOXICACION
description Toxic honeydew produced by Flatidae Epormenis cestri in Uruguay has been shown to cause among honeybees (Apis mellifera) colonies a massive larva death called “River disease”, but the intrinsic mechanisms are still unknown. Because fat body cells, oenocytes and trophocytes, are known to regulated larvae metabolism, and to be affected by xenobiotics, we tested whether apoptosis of these cells can be an underlying cause of larvae death. Ten colonies were divided into two groups and fed with common honey or toxic honeydew obtained from colonies affected by “River disease”. Five-dayold larvae were collected and processed for histology and immunohistochemistry for caspase-3. The area, diameter, and immunostaining area in oenocytes and trophocytes were measured. The oenocyte and trophocyte cellular area decreased in the treated group (p=0.002; p<0.001 respectively) compared to the control group. The diameter of oenocytes (p=0.0002) and trophocytes (p<0.0001) decreased in the treated group. Caspase-3 was detected in cytoplasm in the control group but in the cytoplasm and nucleus in the treated group. The caspase-3 immunostaining area increased in oenocytes (p<0.002) and trophocytes (p<0.0001) of the treated group. The ingestion of toxic honeydew altered the morphology, localization and immunoexpression of caspase-3 in fat body cells, which suggests that the deregulation of the apoptotic mechanism affected the normal development in A. mellifera larvae.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021
dc.date.accessioned.none.fl_str_mv 2025-02-14T17:35:10Z
dc.date.available.none.fl_str_mv 2025-02-14T17:35:10Z
dc.type.es.fl_str_mv Artículo
dc.type.none.fl_str_mv info:eu-repo/semantics/article
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.citation.es.fl_str_mv Viotti Cresci, M, Juri, P, Presentado, M, Verdes García, J, Nogueira, E, Invernizzi, C y Pedrana, G. Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri. Journal of Apicultural Science.[en línea] 2021, 65(1), 101-108
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12008/48404
dc.identifier.doi.none.fl_str_mv 10.2478/jas-2021-0006
identifier_str_mv Viotti Cresci, M, Juri, P, Presentado, M, Verdes García, J, Nogueira, E, Invernizzi, C y Pedrana, G. Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri. Journal of Apicultural Science.[en línea] 2021, 65(1), 101-108
10.2478/jas-2021-0006
url https://hdl.handle.net/20.500.12008/48404
dc.language.iso.none.fl_str_mv en
eng
language_invalid_str_mv en
language eng
dc.relation.none.fl_str_mv Journal of Apicultural Science, 2021, 65(1), 101-108
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.license.none.fl_str_mv Licencia Creative Commons Atribución (CC - By 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv Licencia Creative Commons Atribución (CC - By 4.0)
dc.format.extent.es.fl_str_mv 8 p
dc.format.mimetype.es.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:COLIBRI
instname:Universidad de la República
instacron:Universidad de la República
instname_str Universidad de la República
instacron_str Universidad de la República
institution Universidad de la República
reponame_str COLIBRI
collection COLIBRI
bitstream.url.fl_str_mv http://localhost:8080/xmlui/bitstream/20.500.12008/48404/5/license.txt
http://localhost:8080/xmlui/bitstream/20.500.12008/48404/2/license_url
http://localhost:8080/xmlui/bitstream/20.500.12008/48404/3/license_text
http://localhost:8080/xmlui/bitstream/20.500.12008/48404/4/license_rdf
http://localhost:8080/xmlui/bitstream/20.500.12008/48404/1/V12.pdf
bitstream.checksum.fl_str_mv 6429389a7df7277b72b7924fdc7d47a9
a0ebbeafb9d2ec7cbb19d7137ebc392c
987f1fd600231524e26f581f92a3a278
71ed42ef0a0b648670f707320be37b90
9bb0ea0f95346d3d2487bb7f658395ae
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv COLIBRI - Universidad de la República
repository.mail.fl_str_mv karina.camps@seciu.edu.uy
_version_ 1871461182504697856
spelling Viotti Cresci María Helen, Universidad de la República (Uruguay). Facultad de VeterinariaJuri Pablo, Universidad de la República (Uruguay). Facultad de VeterinariaPresentado María Laura, Universidad de la República (Uruguay). Facultad de VeterinariaVerdes García José Manuel, Universidad de la República (Uruguay). Facultad de VeterinariaNogueira Enrique, Universidad de la República (Uruguay). Facultad de VeterinariaInvernizzi Ciro, Universidad de la República (Uruguay). Facultad de VeterinariaPedrana Graciela, Universidad de la República (Uruguay). Facultad de Veterinaria2025-02-14T17:35:10Z2025-02-14T17:35:10Z2021Viotti Cresci, M, Juri, P, Presentado, M, Verdes García, J, Nogueira, E, Invernizzi, C y Pedrana, G. Increased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestri. Journal of Apicultural Science.[en línea] 2021, 65(1), 101-108https://hdl.handle.net/20.500.12008/4840410.2478/jas-2021-0006Toxic honeydew produced by Flatidae Epormenis cestri in Uruguay has been shown to cause among honeybees (Apis mellifera) colonies a massive larva death called “River disease”, but the intrinsic mechanisms are still unknown. Because fat body cells, oenocytes and trophocytes, are known to regulated larvae metabolism, and to be affected by xenobiotics, we tested whether apoptosis of these cells can be an underlying cause of larvae death. Ten colonies were divided into two groups and fed with common honey or toxic honeydew obtained from colonies affected by “River disease”. Five-dayold larvae were collected and processed for histology and immunohistochemistry for caspase-3. The area, diameter, and immunostaining area in oenocytes and trophocytes were measured. The oenocyte and trophocyte cellular area decreased in the treated group (p=0.002; p<0.001 respectively) compared to the control group. The diameter of oenocytes (p=0.0002) and trophocytes (p<0.0001) decreased in the treated group. Caspase-3 was detected in cytoplasm in the control group but in the cytoplasm and nucleus in the treated group. The caspase-3 immunostaining area increased in oenocytes (p<0.002) and trophocytes (p<0.0001) of the treated group. The ingestion of toxic honeydew altered the morphology, localization and immunoexpression of caspase-3 in fat body cells, which suggests that the deregulation of the apoptotic mechanism affected the normal development in A. mellifera larvae.Submitted by García Alastra Leticia (lgarcia@fvet.edu.uy) on 2025-02-14T17:35:10Z No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) V12.pdf: 654366 bytes, checksum: 9bb0ea0f95346d3d2487bb7f658395ae (MD5)Made available in DSpace by García Alastra Leticia (lgarcia@fvet.edu.uy) on 2025-02-14T17:35:10Z (GMT). No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) V12.pdf: 654366 bytes, checksum: 9bb0ea0f95346d3d2487bb7f658395ae (MD5) Previous issue date: 20218 papplication/pdfenengJournal of Apicultural Science, 2021, 65(1), 101-108Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)ABEJASAPIS MELLIFERAAPICULTURAENFERMEDADESINTOXICACIONIncreased caspase-3 immunoexpression and morphology alterations in oenocytes and trophocytes of Apis mellifera larvae induced by toxic secretion of Epormenis cestriArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaViotti Cresci, María HelenJuri, PabloPresentado, María LauraVerdes García, José ManuelNogueira, EnriqueInvernizzi, CiroPedrana, GracielaLICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/48404/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-844http://localhost:8080/xmlui/bitstream/20.500.12008/48404/2/license_urla0ebbeafb9d2ec7cbb19d7137ebc392cMD52license_textlicense_texttext/html; charset=utf-826974http://localhost:8080/xmlui/bitstream/20.500.12008/48404/3/license_text987f1fd600231524e26f581f92a3a278MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-824251http://localhost:8080/xmlui/bitstream/20.500.12008/48404/4/license_rdf71ed42ef0a0b648670f707320be37b90MD54ORIGINALV12.pdfV12.pdfapplication/pdf654366http://localhost:8080/xmlui/bitstream/20.500.12008/48404/1/V12.pdf9bb0ea0f95346d3d2487bb7f658395aeMD5120.500.12008/484042025-02-14 14:35:10.073oai:colibri.udelar.edu.uy:20.500.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Institucionalhttps://www.colibri.udelar.edu.uyUniversidad públicahttps://udelar.edu.uy/https://www.colibri.udelar.edu.uy/oai/requestkarina.camps@seciu.edu.uyUruguayopendoar:47712025-02-14T17:35:10COLIBRI - Universidad de la Repúblicafalse
score 14.713841

Registos relacionados