Nitrocellulose Membrane-Based Enzyme-Linked Immunoassay for Dengue Serotype-1 IgM Detection
OBJETIVES: To evaluate the sensitivity and specifity of a nitrocellulose membrane-based immunoassay for dengue IgM, with respect to capture enzyme immunoassay, for the diagnosis of dengue virus infection. METHODS: 101 serum samples were processed and divided into 2 groups: 53 from dengue serotype 1...
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 1999 |
| País: | Perú |
| Recursos: | Universidad Nacional Mayor de San Marcos |
| Repositório: | Revistas - Universidad Nacional Mayor de San Marcos |
| Idioma: | espanhol |
| OAI Identifier: | oai:revistasinvestigacion.unmsm.edu.pe:article/4475 |
| Acesso em linha: | https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4475 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Dengue Collodion Immunoassay IgM Colodion Inmunoensayo |
| Resumo: | OBJETIVES: To evaluate the sensitivity and specifity of a nitrocellulose membrane-based immunoassay for dengue IgM, with respect to capture enzyme immunoassay, for the diagnosis of dengue virus infection. METHODS: 101 serum samples were processed and divided into 2 groups: 53 from dengue serotype 1 (DEN1) infected patients, and 48 from healthy subjects. Both groups were tested with a nitrocellulose membrane-based IgM capture enzyme immunoassay (NMB-EIA) and also with an ELISA as referential pattern. RESULTS: NMB-EIA testing detected IgM anti-DEN1 in 94,34% of samples from infected patients, and in 14,58% of control samples, whereas ELISA fails to report false positive or false negative results. CONCLUSION: NMB-EIA appears to be a good alternative for dengue infection diagnosis. |
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