Estudio de la producción y extracción de α-L-fucosidasa de bifidobacterias
The fucosylated human milk oligosaccharides are compounds of interest for their prebiotic action in the intestinal microbiota of infants. That is why there is an increase in research associated with the synthesis of oligosaccharides by different techniques. One way of obtaining is enzymatic synthesi...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:j38606956 |
| Acceso en línea: | https://doi.org/10.24275/uami.j38606956 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Enzymes -- Synthesis info:eu-repo/classification/LEM/Bifidobacterium info:eu-repo/classification/LEM/Oligosaccharides -- Synthesis info:eu-repo/classification/LEM/Enzimas -- Síntesis info:eu-repo/classification/LEM/Oligosacáridos -- Síntesis info:eu-repo/classification/LEM/Bifidobacterias info:eu-repo/classification/cti/6 |
| Sumario: | The fucosylated human milk oligosaccharides are compounds of interest for their prebiotic action in the intestinal microbiota of infants. That is why there is an increase in research associated with the synthesis of oligosaccharides by different techniques. One way of obtaining is enzymatic synthesis by glycosyl hydrolases such as α-L-fucosidase, which is an enzyme found in a wide variety of organisms. Thus, in this work, the objective was to produce and extract α-L-fucosidase from bifidobacteria. For this research, the work is divided into three parts, using three species of bifidobacteria: Bifidobacterium longum, Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum. First, the enzymatic activity of the three bifidobacteria was evaluated, both for the cell associated activity and extracellular activity, finding cell associated activity only. In the second stage, enzyme production was increased by inducing it´s production by using inulin as a sole carbon source in the culture medium, achieving the desired increase in the specific activity of α-L-fucosidase in the three bifidobacteria. Finally, an ultrasound method was performed to perform a partial purification of α-L-fucosidase from Bifidobacterium longum grown in culture medium with inulin, optimizing the extraction using a response surface method with three variables (time, power and volume). Releasing, approximately one third of the cell associated residual activity. With these results it was found that it is possible to increase the activity of α-L-fucosidase and extract and purify the α-Lfucosidase from Bifidobacterium longum. |
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