Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
At present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact...
| Autores: | , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | México |
| Recursos: | UNIVERSIDAD DE SONORA |
| Repositorio: | Epistemus |
| Idioma: | español |
| OAI Identifier: | oai:oai.epistemus.unison.mx:article/97 |
| Acesso em linha: | https://epistemus.unison.mx/index.php/epistemus/article/view/97 |
| Access Level: | acceso abierto |
| Palavra-chave: | Staphylococcus aureus MRSA PCR PCR-RT SARM |
| Resumo: | At present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact that these pathogens have developed resistance to environmental threats. The excessive use of sanitizers and antibiotics such as methicillin are the cause of the resistance that in thiscase comes from the mecA gene. The identification of this gene is crucial to differentiate resistant and non-resistant strains. However, taking into count that traditional processes for the identification of this pathogen are usually very slow, techniques such as PCR are applied because of their efficacy and efficiency. However, techniques such as PCR-RT have improvements that provide some advantage compared to its predecessor. |
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