Susceptibility of Litopenaeus schmitti and Cherax quadricarinatus to white spot syndrome virus (WSSV)

 Bioassays were carried out in a closed system to evaluate the susceptibility of Litopenaeus schmitti and Cherax quadricarinatus to white spot syndrome virus (WSSV) using L. vannamei as the positive control reference species. Animals were injected intramuscularly (20 ∞L shrimp–1) with a WSS...

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Detalhes bibliográficos
Autores: Unzueta-Bustamante, Marco Linné, Silveira-Cofficny, Raquel, Prieto, Adela A, Aguirre-Guzmán, Gabriel, Vázquez-Juárez, Ricardo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:México
Recursos:UNIVERSIDAD AUTÓNOMA DE BAJA CALIFORNIA
Repositorio:Ciencias Marinas
Idioma:inglés
OAI Identifier:oai:cienciasmarinas.com.mx:article/344
Acesso em linha:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/344
Access Level:acceso abierto
Palavra-chave:WSSV
Litopenaeus vannamei
Cherax quadricarinatus
susceptibility
in situ hybridization
susceptibilidad
hibridacion in situ
Descrição
Resumo: Bioassays were carried out in a closed system to evaluate the susceptibility of Litopenaeus schmitti and Cherax quadricarinatus to white spot syndrome virus (WSSV) using L. vannamei as the positive control reference species. Animals were injected intramuscularly (20 ∞L shrimp–1) with a WSSV preparation obtained from the Aquaculture Pathology Laboratory of the University of Arizona. These infected organisms were reared separately in their respective experimental tank. Susceptibility to WSSV was significantly greater in L. vannamei than in the other two species (P < 0.05). The histological analysis showed characteristic inclusion bodies of WSSV in these three crustacean species, with a different distribution of infection depending on the tissue and species. In situ hybridization also showed these signs of infection in hemocytes and hepatopancreas.