THE CARBOXY-TERMINAL TAIL OF THE STE2 RECEPTOR IS INVOLVED IN ACTIVATION OF THE G PROTEIN IN THE SACCHAROMYCES CEREVISIAE Α-PHEROMONE RESPONSE PATHWAY
The Ste2 gene encodes the yeast K-pheromone receptor that belongs to the superfamily of seven-transmembrane G protein-coupled receptors. Binding of pheromone induces activation of the heterotrimeric G protein triggering growth arrest in G1 phase and induction of genes required for mating. By random...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2001 |
| País: | México |
| Recursos: | Universidad Autónoma de Nayarit |
| Repositorio: | Repositorio Institucional Aramara de la UAN |
| Idioma: | inglés |
| OAI Identifier: | oai:dspace.uan.mx:123456789/1964 |
| Acesso em linha: | https://doi.org/10.1111/j.1574-6968.2001.tb10584.x http://dspace.uan.mx:8080/jspui/handle/123456789/1964 |
| Access Level: | acceso abierto |
| Palavra-chave: | Ste2 C-terminal Proteína G Transducción de señales Estructura-función Levadura C-terminus G protein Signal transduction Structure-function Yeast Saccharomyces cerevisiae MEDICINA Y CIENCIAS DE LA SALUD [3] |
| Resumo: | The Ste2 gene encodes the yeast K-pheromone receptor that belongs to the superfamily of seven-transmembrane G protein-coupled receptors. Binding of pheromone induces activation of the heterotrimeric G protein triggering growth arrest in G1 phase and induction of genes required for mating. By random PCR-mediated mutagenesis we isolated mutant 8L4, which presents a substitution of an asparagine residue by serine at position 388 of the K-factor receptor. The 8L4 mutant strain shows phenotypic defects such as: reduction in growth arrest after pheromone treatment, diminished activation of the Fus1 gene, and impaired mating competence. The asparagine residue lies in the second half of the intracellular protruding C-terminal tail of the receptor, and its replacement by serine affects interaction with both the GK and GL subunits. Since expression of the receptor as well as its kinetic parameters, i.e., ligand affinity and receptor number, are unaffected in the mutant strain, we propose that association of the C-terminal tail of the receptor with GK and GL subunits is required for proper activation of the heterotrimeric G protein. Besides its described role in downregulation and in formation of preactivation complex, the results here shown indicate that the C-terminal tail of the receptor plays an active role in transmitting the stimulus of mating pheromone to the heterotrimeric G protein. |
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