Heterologous gene expression in an Escherichia coli population under starvation stress conditions
A novel system to study the evolution of transcription signals in heterologous systems under selective starvation conditions is described. It is based on the plasmid-mediated transfer of his biosynthetic genes from Azospirillum brasilense into a heterologous Escherichia coli mutant population lackin...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1998 |
| País: | México |
| Recursos: | Universidad Nacional Autónoma de México |
| Repositorio: | Sistema de Información de la Facultad de Ciencias, UNAM |
| OAI Identifier: | oai:repositorio.fciencias.unam.mx:11154/3333 |
| Acesso em linha: | http://hdl.handle.net/11154/3333 |
| Access Level: | acceso abierto |
| Palavra-chave: | Biochemistry & Molecular Biology Evolutionary Biology Genetics & Heredity starvation conditions promoter-creating mutations heterologous gene transfer metabolic evolution adaptive mutations |
| Resumo: | A novel system to study the evolution of transcription signals in heterologous systems under selective starvation conditions is described. It is based on the plasmid-mediated transfer of his biosynthetic genes from Azospirillum brasilense into a heterologous Escherichia coli mutant population lacking histidine biosynthetic ability. We show that under highly selective stressful conditions, genetic changes in the donor plasmid lead to mutated sequences that are efficiently recognized as promoters by the E. coli RNA polymerase. |
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