Ovicidal activity of extracts from four plant species against the cattle nematode Cooperia punctata
Bioactive plants might represent an alternative for Cooperia punctata control in grazing cattle. The objectives of this study were (1) to assess the ovicidal activity of extracts from 4 plant species against C. punctata , (2) to determine the role of the polyphenols in the plants’ anthelmintic (AH)...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | México |
| Institución: | Universidad Nacional Autónoma de México |
| Repositorio: | Redalyc-UNAM |
| OAI Identifier: | oai:redalyc.org:493550289003 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=493550289003 |
| Access Level: | acceso abierto |
| Palabra clave: | Veterinaria Cattle Nematodes Plant extracts Ovicidal effect Cooperia punctata |
| Sumario: | Bioactive plants might represent an alternative for Cooperia punctata control in grazing cattle. The objectives of this study were (1) to assess the ovicidal activity of extracts from 4 plant species against C. punctata , (2) to determine the role of the polyphenols in the plants’ anthelmintic (AH) activity, and (3) to evaluate the best plant extraction procedure when searching for ovicidal activity. The egg hatch assay was used with different extraction procedures, aqueous (AQ), acetone:water (AW) and acetonic (AC), to evaluate the ovicid - al activity of Leucaena leucocephala , Gliricidia sepium , Guazuma ulmifolia and Cratylia argentea . Eggs of C. punctata were exposed in quadruplicate to 0.6, 1.2, 2.4, 4.8 and 9.6 mg mL -1 of each plant extract. The roles of the polyphenols were assessed using polyethylene glycol (PEG). The 12 plant extracts inhibited egg hatching in a dose-dependent manner. Best-fit LC 50 values were 1.03 ± 0.17 and 7.90 ± 1.19 mg mL -1 for G. sepium -AC and L. leucocephala -AQ, respectively. Differences in AH activity were found among the extraction procedures (P < 0.05). At the highest concentration, L. leu - cocephala -AQ inhibited more than 50% of C. punctata hatching. The G. sepium -AC extract fully inhibited egg hatching. The addition of polyethylene glycol revealed the role of the polyphenols in the bioactivity of most plant extracts; however, for G. sepium -AC, the polyphenols were not the main bio - active compounds. Overall, acetone:water extraction represented the best extraction procedure to obtain both ovicidal activity and higher yield. The inhibition rates suggested that L. leucocephala and G. sepium should be evaluated as a means of reducing larval density in pastures. |
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