Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes

This article belongs to the Special Issue Innovative Molecular Target and Therapeutic Approaches in Nonalcoholic Fatty Liver Disease/Nonalcoholic Steatohepatitis (NAFLD/NASH).

Detalhes bibliográficos
Autores: Carvajal, Silvia, Perramón, Meritxell, Casals, Gregori, Oró, Denise, Ribera, Jordi, Morales-Ruiz, Manuel, Casals, Eudald, Casado, Pedro, Melgar-Lesmes, Pedro, Fernández-Varo, Guillermo, Cutillas, Pedro, Puntes, Víctor F., Jiménez, Wladimiro
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/209051
Acesso em linha:http://hdl.handle.net/10261/209051
Access Level:Acceso aberto
Palavra-chave:Cerium oxide nanoparticles
Phosphoproteomics
Oxidative stress
Human hepatic cells
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dc.title.none.fl_str_mv Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
spellingShingle Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
Carvajal, Silvia
Cerium oxide nanoparticles
Phosphoproteomics
Oxidative stress
Human hepatic cells
title_short Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title_full Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title_fullStr Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title_full_unstemmed Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
title_sort Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytes
dc.creator.none.fl_str_mv Carvajal, Silvia
Perramón, Meritxell
Casals, Gregori
Oró, Denise
Ribera, Jordi
Morales-Ruiz, Manuel
Casals, Eudald
Casado, Pedro
Melgar-Lesmes, Pedro
Fernández-Varo, Guillermo
Cutillas, Pedro
Puntes, Víctor F.
Jiménez, Wladimiro
author Carvajal, Silvia
author_facet Carvajal, Silvia
Perramón, Meritxell
Casals, Gregori
Oró, Denise
Ribera, Jordi
Morales-Ruiz, Manuel
Casals, Eudald
Casado, Pedro
Melgar-Lesmes, Pedro
Fernández-Varo, Guillermo
Cutillas, Pedro
Puntes, Víctor F.
Jiménez, Wladimiro
author_role author
author2 Perramón, Meritxell
Casals, Gregori
Oró, Denise
Ribera, Jordi
Morales-Ruiz, Manuel
Casals, Eudald
Casado, Pedro
Melgar-Lesmes, Pedro
Fernández-Varo, Guillermo
Cutillas, Pedro
Puntes, Víctor F.
Jiménez, Wladimiro
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Generalitat de Catalunya
European Commission
Fundació La Marató de TV3
Wuyi University
Natural Science Foundation of Guangdong Province
Instituto de Salud Carlos III
Perramón, Meritxell [0000-0002-1944-8337]
Casals, Gregori [0000-0002-3271-1371]
Ribera, Jordi [0000-0002-0288-4220]
Casals, Eudald [0000-0002-2900-7295]
Jiménez, Wladimiro [0000-0002-9376-0214]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cerium oxide nanoparticles
Phosphoproteomics
Oxidative stress
Human hepatic cells
topic Cerium oxide nanoparticles
Phosphoproteomics
Oxidative stress
Human hepatic cells
description This article belongs to the Special Issue Innovative Molecular Target and Therapeutic Approaches in Nonalcoholic Fatty Liver Disease/Nonalcoholic Steatohepatitis (NAFLD/NASH).
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/209051
url http://hdl.handle.net/10261/209051
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094734-B-C21
RTI2018-094734-B-C21/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75358-R
https://doi.org/10.3390/ijms20235959
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Cerium oxide nanoparticles protect against oxidant injury and interfere with oxidative mediated kinase signaling in human-derived hepatocytesCarvajal, SilviaPerramón, MeritxellCasals, GregoriOró, DeniseRibera, JordiMorales-Ruiz, ManuelCasals, EudaldCasado, PedroMelgar-Lesmes, PedroFernández-Varo, GuillermoCutillas, PedroPuntes, Víctor F.Jiménez, WladimiroCerium oxide nanoparticlesPhosphoproteomicsOxidative stressHuman hepatic cellsThis article belongs to the Special Issue Innovative Molecular Target and Therapeutic Approaches in Nonalcoholic Fatty Liver Disease/Nonalcoholic Steatohepatitis (NAFLD/NASH).Cerium oxide nanoparticles (CeO2NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO2NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO2NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO2NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO2NPs did not modify HepG2 cell viability in basal conditions but reduced H2O2- and lipopolysaccharide (LPS)-induced cell death and prevented H2O2-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO2NPs reverted the H2O2-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H2O2 effects on mTOR, MAPK/ERK, CK2A1 and PKACA signaling pathways. In conclusion, CeO2NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways.This research was funded by grants to W. Jiménez from Ministerio de Economia y Competitividad [grants, SAF2015-64126-R, RTI2018-094734-B-C21], to M. Morales-Ruiz [grant SAF2016-75358-R] and to G. Casals [grant PI-15/00777]; cofinanced by FEDER, European Union, a way of making Europe, Agència de Gestió d’Ajuts Universitaris i de Recerca [grant SGR 2014/219]; and Fundació La Marató de TV3 [grant Marató 120930];Wuyi University Funding for Hight Talents Introduction, grant number 2018TP010 and Foundation from Department of Education of Guangdong Province, grant number 2016KCXTD005 and 2017KSYS010, to Eudald Casals The Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd) is funded by the Instituto de Salud Carlos III.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Generalitat de CatalunyaEuropean CommissionFundació La Marató de TV3Wuyi UniversityNatural Science Foundation of Guangdong ProvinceInstituto de Salud Carlos IIIPerramón, Meritxell [0000-0002-1944-8337]Casals, Gregori [0000-0002-3271-1371]Ribera, Jordi [0000-0002-0288-4220]Casals, Eudald [0000-0002-2900-7295]Jiménez, Wladimiro [0000-0002-9376-0214]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/209051reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2015-64126-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094734-B-C21RTI2018-094734-B-C21/AEI/10.13039/501100011033info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75358-Rhttps://doi.org/10.3390/ijms20235959Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2090512026-05-22T06:33:51Z
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