A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome

The microbiome plays a key role in homeostasis and health and it has been also linked to fertility and semen quality in several animal species including swine. Despite the more than likely importance of sperm bacteria on the boar’s reproductive ability and the dissemination of pathogens and antimicr...

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Detalles Bibliográficos
Autores: Gòdia, Marta, Ramayo-Caldas, Yuliaxis, Zingaretti, Laura M., Darwich, Laila, López, Samantha, Rodríguez-Gil, Joan E., Yeste, Marc, Sánchez, Armand, Clop, Alex
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/228977
Acceso en línea:http://hdl.handle.net/10261/228977
Access Level:acceso abierto
Palabra clave:Spermatozoa
Microbiome
Pig
Sperm quality
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spelling A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiomeGòdia, MartaRamayo-Caldas, YuliaxisZingaretti, Laura M.Darwich, LailaLópez, SamanthaRodríguez-Gil, Joan E.Yeste, MarcSánchez, ArmandClop, AlexSpermatozoaMicrobiomePigSperm qualityThe microbiome plays a key role in homeostasis and health and it has been also linked to fertility and semen quality in several animal species including swine. Despite the more than likely importance of sperm bacteria on the boar’s reproductive ability and the dissemination of pathogens and antimicrobial resistance genes, the high throughput characterization of the swine sperm microbiome remains scarce. We carried RNA-seq on 40 ejaculates each from a different Pietrain boar and found that a proportion of the sequencing reads did not map to the Sus scrofa genome. The current study aimed at using these reads not belonging to pig to carry a pilot study to profile the boar sperm bacterial population and its relation with 7 semen quality traits. We found that the boar sperm contains a broad population of bacteria. The most abundant phyla were Proteobacteria (39.1%), Firmicutes (27.5%), Actinobacteria (14.9%) and Bacteroidetes (5.7%). The predominant species contaminated sperm after ejaculation from soil, faeces and water sources (Bacillus megaterium, Brachybacterium faecium, Bacillus coagulans). Some potential pathogens were also found but at relatively low levels (Escherichia coli, Clostridioides difficile, Clostridium perfringens, Clostridium botulinum and Mycobacterium tuberculosis). We also identified 3 potential antibiotic resistant genes from E. coli against chloramphenicol, Neisseria meningitidis against spectinomycin and Staphylococcus aureus against linezolid. None of these genes were highly abundant. Finally, we classified the ejaculates into categories according to their bacterial features and semen quality parameters and identified two categories that significantly differed for 5 semen quality traits and 13 bacterial features including the genera Acinetobacter, Stenotrophomonas and Rhodobacter. Our results show that boar semen contains a bacterial community, including potential pathogens and putative antibiotic resistance genes, and that these bacteria may affect its reproductive performance.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/228977reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.theriogenology.2020.08.001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2289772026-05-22T06:33:51Z
dc.title.none.fl_str_mv A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome
title A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome
spellingShingle A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome
Gòdia, Marta
Spermatozoa
Microbiome
Pig
Sperm quality
title_short A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome
title_full A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome
title_fullStr A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome
title_full_unstemmed A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome
title_sort A pilot RNA-seq study in 40 pietrain ejaculates to characterize the porcine sperm microbiome
dc.creator.none.fl_str_mv Gòdia, Marta
Ramayo-Caldas, Yuliaxis
Zingaretti, Laura M.
Darwich, Laila
López, Samantha
Rodríguez-Gil, Joan E.
Yeste, Marc
Sánchez, Armand
Clop, Alex
author Gòdia, Marta
author_facet Gòdia, Marta
Ramayo-Caldas, Yuliaxis
Zingaretti, Laura M.
Darwich, Laila
López, Samantha
Rodríguez-Gil, Joan E.
Yeste, Marc
Sánchez, Armand
Clop, Alex
author_role author
author2 Ramayo-Caldas, Yuliaxis
Zingaretti, Laura M.
Darwich, Laila
López, Samantha
Rodríguez-Gil, Joan E.
Yeste, Marc
Sánchez, Armand
Clop, Alex
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Spermatozoa
Microbiome
Pig
Sperm quality
topic Spermatozoa
Microbiome
Pig
Sperm quality
description The microbiome plays a key role in homeostasis and health and it has been also linked to fertility and semen quality in several animal species including swine. Despite the more than likely importance of sperm bacteria on the boar’s reproductive ability and the dissemination of pathogens and antimicrobial resistance genes, the high throughput characterization of the swine sperm microbiome remains scarce. We carried RNA-seq on 40 ejaculates each from a different Pietrain boar and found that a proportion of the sequencing reads did not map to the Sus scrofa genome. The current study aimed at using these reads not belonging to pig to carry a pilot study to profile the boar sperm bacterial population and its relation with 7 semen quality traits. We found that the boar sperm contains a broad population of bacteria. The most abundant phyla were Proteobacteria (39.1%), Firmicutes (27.5%), Actinobacteria (14.9%) and Bacteroidetes (5.7%). The predominant species contaminated sperm after ejaculation from soil, faeces and water sources (Bacillus megaterium, Brachybacterium faecium, Bacillus coagulans). Some potential pathogens were also found but at relatively low levels (Escherichia coli, Clostridioides difficile, Clostridium perfringens, Clostridium botulinum and Mycobacterium tuberculosis). We also identified 3 potential antibiotic resistant genes from E. coli against chloramphenicol, Neisseria meningitidis against spectinomycin and Staphylococcus aureus against linezolid. None of these genes were highly abundant. Finally, we classified the ejaculates into categories according to their bacterial features and semen quality parameters and identified two categories that significantly differed for 5 semen quality traits and 13 bacterial features including the genera Acinetobacter, Stenotrophomonas and Rhodobacter. Our results show that boar semen contains a bacterial community, including potential pathogens and putative antibiotic resistance genes, and that these bacteria may affect its reproductive performance.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/228977
url http://hdl.handle.net/10261/228977
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.theriogenology.2020.08.001
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.228081

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