In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity

This study investigated the impact of a novel passion fruit peel–extracted pectin (PFPEP) with and without probiotics on the human intestinal microbiota composition and metabolic activity, compared to commercial pectin (CP). Independent batch fermentations were conducted using fecal samples from fou...

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Autores: Pimisa, Rattanaporn, Prasongsuk, Sehanat, Bankeeree, Wichanee, Pongcharoen, Pongsanat, Arboleya, Silvia, Nogacka, Alicja, González de los Reyes-Gavilán, Clara, Hwanhlem, Noraphat, Gueimonde Fernández, Miguel
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376349
Acesso em linha:http://hdl.handle.net/10261/376349
https://api.elsevier.com/content/abstract/scopus_id/85209732851
Access Level:Acceso aberto
Palavra-chave:Fecal microbiota
Pectin
Prebiotic
Probiotic
Short chain fatty acids
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spelling In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activityPimisa, RattanapornPrasongsuk, SehanatBankeeree, WichaneePongcharoen, PongsanatArboleya, SilviaNogacka, AlicjaGonzález de los Reyes-Gavilán, ClaraHwanhlem, NoraphatGueimonde Fernández, MiguelFecal microbiotaPectinPrebioticProbioticShort chain fatty acidsThis study investigated the impact of a novel passion fruit peel–extracted pectin (PFPEP) with and without probiotics on the human intestinal microbiota composition and metabolic activity, compared to commercial pectin (CP). Independent batch fermentations were conducted using fecal samples from four healthy donors aged between 25 and 40 years to assess changes in pH, gas accumulation, microbiota composition, short–chain fatty acids (SCFAs) production, and metabolic activity. Our findings revealed distinct responses to pectin types and probiotic strains. The fecal culture supplemented with CP resulted in a rapid drop in pH and promoted higher butyrate levels, as well as exhibited greater glucose content than PFPEP before fermentation. Conversely, PFPEP with and without probiotics stimulated large levels of acetate production. Whereas, the fermentation of PFPEP, with and without probiotics added, or CP showed similar results, a higher level of cumulative gas, an increase in levels of Erysipelotrichaceae, Coriobacteriaceae, and Bifidobacteriaceae families, a reduced in Bacteroidaceae and Prevotellaceae families and Bacteroides spp., and significant changes in metabolic activity were found. These results underscore the potential of PFPEP for modulating gut microbiota, guiding avenues for further research of this ingredient in human intervention studies.This work was financially supported by the National Research Council of Thailand (NRCT) and the Thailand Science Research and Innovation under the Program of Research and Researchers for Industries (RRi) [Grant Number: PHD62I0013]. We thank Khaokor Agro–Industry Co., Ltd., Khao Kho, Phetchabun, Thailand, for the passion fruit peel samples. It also acknowledges the Scientific & Technology Services of IPLA-CSIC, for their excellent assistance.Peer reviewedElsevier BVNational Research Council of ThailandThailand Science Research and InnovationGueimonde Fernández, Miguel [0000-0002-0192-901X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/376349https://api.elsevier.com/content/abstract/scopus_id/85209732851reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.lwt.2024.117082https://doi.org/10.1016/j.lwt.2024.117082Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3763492026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity
title In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity
spellingShingle In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity
Pimisa, Rattanaporn
Fecal microbiota
Pectin
Prebiotic
Probiotic
Short chain fatty acids
title_short In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity
title_full In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity
title_fullStr In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity
title_full_unstemmed In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity
title_sort In vitro assessment of the impact of passion fruit peel–extracted pectin added with probiotic strains on the human intestinal microbiota and metabolic activity
dc.creator.none.fl_str_mv Pimisa, Rattanaporn
Prasongsuk, Sehanat
Bankeeree, Wichanee
Pongcharoen, Pongsanat
Arboleya, Silvia
Nogacka, Alicja
González de los Reyes-Gavilán, Clara
Hwanhlem, Noraphat
Gueimonde Fernández, Miguel
author Pimisa, Rattanaporn
author_facet Pimisa, Rattanaporn
Prasongsuk, Sehanat
Bankeeree, Wichanee
Pongcharoen, Pongsanat
Arboleya, Silvia
Nogacka, Alicja
González de los Reyes-Gavilán, Clara
Hwanhlem, Noraphat
Gueimonde Fernández, Miguel
author_role author
author2 Prasongsuk, Sehanat
Bankeeree, Wichanee
Pongcharoen, Pongsanat
Arboleya, Silvia
Nogacka, Alicja
González de los Reyes-Gavilán, Clara
Hwanhlem, Noraphat
Gueimonde Fernández, Miguel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Research Council of Thailand
Thailand Science Research and Innovation
Gueimonde Fernández, Miguel [0000-0002-0192-901X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fecal microbiota
Pectin
Prebiotic
Probiotic
Short chain fatty acids
topic Fecal microbiota
Pectin
Prebiotic
Probiotic
Short chain fatty acids
description This study investigated the impact of a novel passion fruit peel–extracted pectin (PFPEP) with and without probiotics on the human intestinal microbiota composition and metabolic activity, compared to commercial pectin (CP). Independent batch fermentations were conducted using fecal samples from four healthy donors aged between 25 and 40 years to assess changes in pH, gas accumulation, microbiota composition, short–chain fatty acids (SCFAs) production, and metabolic activity. Our findings revealed distinct responses to pectin types and probiotic strains. The fecal culture supplemented with CP resulted in a rapid drop in pH and promoted higher butyrate levels, as well as exhibited greater glucose content than PFPEP before fermentation. Conversely, PFPEP with and without probiotics stimulated large levels of acetate production. Whereas, the fermentation of PFPEP, with and without probiotics added, or CP showed similar results, a higher level of cumulative gas, an increase in levels of Erysipelotrichaceae, Coriobacteriaceae, and Bifidobacteriaceae families, a reduced in Bacteroidaceae and Prevotellaceae families and Bacteroides spp., and significant changes in metabolic activity were found. These results underscore the potential of PFPEP for modulating gut microbiota, guiding avenues for further research of this ingredient in human intervention studies.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/376349
https://api.elsevier.com/content/abstract/scopus_id/85209732851
url http://hdl.handle.net/10261/376349
https://api.elsevier.com/content/abstract/scopus_id/85209732851
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.lwt.2024.117082
https://doi.org/10.1016/j.lwt.2024.117082
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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