The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles
The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA or kDNA or mtDNA, consists of a few maxicircles and thousands of minicircles concatenated together into a huge complex network. These structures present species-specific sizes, from 20 to 40 Kb in maxicircles and from 0.5 to 10 K...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/270273 |
| Acceso en línea: | http://hdl.handle.net/10261/270273 |
| Access Level: | acceso abierto |
| Palabra clave: | Kinetoplast DNA Maxicircle Minicircle Strain Trypanosoma cruzi |
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The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and MinicirclesCallejas-Hernández, FranciscoHerreros-Cabello, AlfonsoDel Moral-Salmoral, JavierFresno, ManuelGironès, NúriaKinetoplast DNAMaxicircleMinicircleStrainTrypanosoma cruziThe mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA or kDNA or mtDNA, consists of a few maxicircles and thousands of minicircles concatenated together into a huge complex network. These structures present species-specific sizes, from 20 to 40 Kb in maxicircles and from 0.5 to 10 Kb in minicircles. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles contain coding guide RNAs involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. The knowledge about this mitochondrial genome is especially relevant since the expression of nuclear and mitochondrial genes involved in oxidative phosphorylation must be coordinated. In Trypanosoma cruzi (T. cruzi), the mtDNA has a dual relevance; the production of energy, and its use as a phylogenetic marker due to its high conservation among strains. Therefore, this study aimed to assemble, annotate, and analyze the complete repertoire of maxicircle and minicircle sequences of different T. cruzi strains by using DNA sequencing. We assembled and annotated the complete maxicircle sequence of the Y and Bug2148 strains. For Bug2148, our results confirm that the maxicircle sequence is the longest assembled to date, and is composed of 21 genes, most of them conserved among Trypanosomatid species. In agreement with previous results, T. cruzi minicircles show a conserved structure around 1.4 Kb, with four highly conserved regions and other four hypervariable regions interspersed between them. However, our results suggest that the parasite minicircles display several sizes and numbers of conserved and hypervariable regions, contrary to those previous studies. Besides, this heterogeneity is also reflected in the three conserved sequence blocks of the conserved regions that play a key role in the minicircle replication. Our results using sequencing technologies of second and third-generation indicate that the different consensus sequences of the maxicircles and minicircles seem to be more complex than previously described indicating at least four different groups in T. cruzi minicircles.Ministerio de Economı́a y competitividad” and “Fondo Europeo de Desarrollo Regional” (SAF2015-63868-R (MINECO/FEDER) to NG, and SAF2016-75988-R (MINECO/FEDER) to MF); “Ministerio de Ciencia, Innovación y Universidades-Agencia Estatal de Investigación” and “Fondo Europeo de Desarrollo Regional” (PGC2018-096132-B-I00 (MICINN/FEDER) to NG); “Red de Investigación de Centros de Enfermedades Tropicales” (RICETRD12/0018/0004 to MF); Comunidad de Madrid (S-2010/BMD-2332 to MF); Consejo Nacional de Ciencia y Tecnologı́a” (CONACYT, Mexico) and the “Consejo de Ciencia, Tecnologı́a e Innovación de Hidalgo” (CITNOVA, Mexico)); AH-C was recipient of a FPU contract of the “Ministerio de Ciencia, Innovación y Universidades and “Fundación Ramón Areces” and “Banco de SantanderMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Red de Investigación Cooperativa en Enfermedades Tropicales (España)Comunidad de MadridConsejo Nacional de Ciencia y Tecnología (México)Consejo de Ciencia y Tecnología e Innovación de HidalgoFundación Ramón ArecesBanco SantanderConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/270273reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3389/fcimb.2021.672448Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2702732026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles |
| title |
The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles |
| spellingShingle |
The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles Callejas-Hernández, Francisco Kinetoplast DNA Maxicircle Minicircle Strain Trypanosoma cruzi |
| title_short |
The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles |
| title_full |
The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles |
| title_fullStr |
The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles |
| title_full_unstemmed |
The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles |
| title_sort |
The Complete Mitochondrial DNA of Trypanosoma cruzi: Maxicircles and Minicircles |
| dc.creator.none.fl_str_mv |
Callejas-Hernández, Francisco Herreros-Cabello, Alfonso Del Moral-Salmoral, Javier Fresno, Manuel Gironès, Núria |
| author |
Callejas-Hernández, Francisco |
| author_facet |
Callejas-Hernández, Francisco Herreros-Cabello, Alfonso Del Moral-Salmoral, Javier Fresno, Manuel Gironès, Núria |
| author_role |
author |
| author2 |
Herreros-Cabello, Alfonso Del Moral-Salmoral, Javier Fresno, Manuel Gironès, Núria |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) Red de Investigación Cooperativa en Enfermedades Tropicales (España) Comunidad de Madrid Consejo Nacional de Ciencia y Tecnología (México) Consejo de Ciencia y Tecnología e Innovación de Hidalgo Fundación Ramón Areces Banco Santander Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Kinetoplast DNA Maxicircle Minicircle Strain Trypanosoma cruzi |
| topic |
Kinetoplast DNA Maxicircle Minicircle Strain Trypanosoma cruzi |
| description |
The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA or kDNA or mtDNA, consists of a few maxicircles and thousands of minicircles concatenated together into a huge complex network. These structures present species-specific sizes, from 20 to 40 Kb in maxicircles and from 0.5 to 10 Kb in minicircles. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles contain coding guide RNAs involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. The knowledge about this mitochondrial genome is especially relevant since the expression of nuclear and mitochondrial genes involved in oxidative phosphorylation must be coordinated. In Trypanosoma cruzi (T. cruzi), the mtDNA has a dual relevance; the production of energy, and its use as a phylogenetic marker due to its high conservation among strains. Therefore, this study aimed to assemble, annotate, and analyze the complete repertoire of maxicircle and minicircle sequences of different T. cruzi strains by using DNA sequencing. We assembled and annotated the complete maxicircle sequence of the Y and Bug2148 strains. For Bug2148, our results confirm that the maxicircle sequence is the longest assembled to date, and is composed of 21 genes, most of them conserved among Trypanosomatid species. In agreement with previous results, T. cruzi minicircles show a conserved structure around 1.4 Kb, with four highly conserved regions and other four hypervariable regions interspersed between them. However, our results suggest that the parasite minicircles display several sizes and numbers of conserved and hypervariable regions, contrary to those previous studies. Besides, this heterogeneity is also reflected in the three conserved sequence blocks of the conserved regions that play a key role in the minicircle replication. Our results using sequencing technologies of second and third-generation indicate that the different consensus sequences of the maxicircles and minicircles seem to be more complex than previously described indicating at least four different groups in T. cruzi minicircles. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/270273 |
| url |
http://hdl.handle.net/10261/270273 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
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http://dx.doi.org/10.3389/fcimb.2021.672448 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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