YRNAs overexpression and potential implications in allergy

[EN] Background Small non-coding RNAs (snRNAs) develop important functions related to epigenetic regulation. YRNAs are snRNAs involved in the initiation of DNA replication and RNA stability that regulate gene expression. They have been related to autoimmune, cancer and inflammatory diseases but neve...

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Authors: Isidoro García, María, García Sánchez, María Asunción, Sanz Lozano, Catalina Sofía, Estravís Satre, Miguel, Marcos Vadillo, Elena, Pascual, Marien, Roa, Sergio, Marqués-García, Fernando, Triviño, Juan Carlos, Dávila González, Ignacio Jesús
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Universidad de Salamanca (USAL)
Repository:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/158564
Online Access:http://hdl.handle.net/10366/158564
Access Level:Open access
Keyword:Allergy
Epigenetics
Expression
Transcriptomics
YRNAs
ncRNA
Genetics
Allergy and Immunology
3207.01 Alergias
2409 Genética
alergia e inmunología
genética
id ES_fa97569d0f3b88ab8f05f36a391273ce
oai_identifier_str oai:gredos.usal.es:10366/158564
network_acronym_str ES
network_name_str España
repository_id_str
spelling YRNAs overexpression and potential implications in allergyIsidoro García, MaríaGarcía Sánchez, María AsunciónSanz Lozano, Catalina SofíaEstravís Satre, MiguelMarcos Vadillo, ElenaPascual, MarienRoa, SergioMarqués-García, FernandoTriviño, Juan CarlosDávila González, Ignacio JesúsAllergyEpigeneticsExpressionTranscriptomicsYRNAsncRNAGeneticsAllergy and Immunology3207.01 Alergias2409 Genéticaalergia e inmunologíagenética[EN] Background Small non-coding RNAs (snRNAs) develop important functions related to epigenetic regulation. YRNAs are snRNAs involved in the initiation of DNA replication and RNA stability that regulate gene expression. They have been related to autoimmune, cancer and inflammatory diseases but never before to allergy. In this work we described for the first time in allergic patients the differential expression profile of YRNAs, their regulatory mechanisms and their potential as new diagnostic and therapeutic targets. Methods From a previous whole RNAseq study in B cells of allergic patients, differential expression profiles of coding and non-coding transcripts were obtained. To select the most differentially expressed non coding transcripts, fold change and p-values were analyzed. A validation of the expression differences detected was developed in an independent cohort of 304 individuals, 208 allergic patients and 96 controls by using qPCR. Potential binding and retrotransponibility capacity were characterized by in silico structural analysis. Using a novel bioinformatics approach, RNA targets identification, functional enrichment and network analyses were performed. Results We found that almost 70% of overexpressed non-coding transcripts in allergic patients corresponded to YRNAs. From the three more differentially overexpressed candidates, increased expression was independently confirmed in the peripheral blood of allergic patients. Structural analysis suggested a protein binding capacity decrease and an increase in retrotransponibility. Studies of RNA targets allowed the identification of sequences related to the immune mechanisms underlying allergy. Conclusions Overexpression of YRNAs is observed for the first time in allergic patients. Structural and functional information points to their implication on regulatory mechanisms of the disease.Instituto de Salud Carlos III; European Regional Development Fund; Junta de Castilla y León; Consolidated Research Unit of Castilla y León; Ramón y Cajal program; FEDER/Ministerio de Ciencia, Innovación y Universidades - Agencia Estatal de InvestigaciónElservier202420242019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/158564reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 InternacionalAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1585642026-06-07T06:28:51Z
dc.title.none.fl_str_mv YRNAs overexpression and potential implications in allergy
title YRNAs overexpression and potential implications in allergy
spellingShingle YRNAs overexpression and potential implications in allergy
Isidoro García, María
Allergy
Epigenetics
Expression
Transcriptomics
YRNAs
ncRNA
Genetics
Allergy and Immunology
3207.01 Alergias
2409 Genética
alergia e inmunología
genética
title_short YRNAs overexpression and potential implications in allergy
title_full YRNAs overexpression and potential implications in allergy
title_fullStr YRNAs overexpression and potential implications in allergy
title_full_unstemmed YRNAs overexpression and potential implications in allergy
title_sort YRNAs overexpression and potential implications in allergy
dc.creator.none.fl_str_mv Isidoro García, María
García Sánchez, María Asunción
Sanz Lozano, Catalina Sofía
Estravís Satre, Miguel
Marcos Vadillo, Elena
Pascual, Marien
Roa, Sergio
Marqués-García, Fernando
Triviño, Juan Carlos
Dávila González, Ignacio Jesús
author Isidoro García, María
author_facet Isidoro García, María
García Sánchez, María Asunción
Sanz Lozano, Catalina Sofía
Estravís Satre, Miguel
Marcos Vadillo, Elena
Pascual, Marien
Roa, Sergio
Marqués-García, Fernando
Triviño, Juan Carlos
Dávila González, Ignacio Jesús
author_role author
author2 García Sánchez, María Asunción
Sanz Lozano, Catalina Sofía
Estravís Satre, Miguel
Marcos Vadillo, Elena
Pascual, Marien
Roa, Sergio
Marqués-García, Fernando
Triviño, Juan Carlos
Dávila González, Ignacio Jesús
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Allergy
Epigenetics
Expression
Transcriptomics
YRNAs
ncRNA
Genetics
Allergy and Immunology
3207.01 Alergias
2409 Genética
alergia e inmunología
genética
topic Allergy
Epigenetics
Expression
Transcriptomics
YRNAs
ncRNA
Genetics
Allergy and Immunology
3207.01 Alergias
2409 Genética
alergia e inmunología
genética
description [EN] Background Small non-coding RNAs (snRNAs) develop important functions related to epigenetic regulation. YRNAs are snRNAs involved in the initiation of DNA replication and RNA stability that regulate gene expression. They have been related to autoimmune, cancer and inflammatory diseases but never before to allergy. In this work we described for the first time in allergic patients the differential expression profile of YRNAs, their regulatory mechanisms and their potential as new diagnostic and therapeutic targets. Methods From a previous whole RNAseq study in B cells of allergic patients, differential expression profiles of coding and non-coding transcripts were obtained. To select the most differentially expressed non coding transcripts, fold change and p-values were analyzed. A validation of the expression differences detected was developed in an independent cohort of 304 individuals, 208 allergic patients and 96 controls by using qPCR. Potential binding and retrotransponibility capacity were characterized by in silico structural analysis. Using a novel bioinformatics approach, RNA targets identification, functional enrichment and network analyses were performed. Results We found that almost 70% of overexpressed non-coding transcripts in allergic patients corresponded to YRNAs. From the three more differentially overexpressed candidates, increased expression was independently confirmed in the peripheral blood of allergic patients. Structural analysis suggested a protein binding capacity decrease and an increase in retrotransponibility. Studies of RNA targets allowed the identification of sequences related to the immune mechanisms underlying allergy. Conclusions Overexpression of YRNAs is observed for the first time in allergic patients. Structural and functional information points to their implication on regulatory mechanisms of the disease.
publishDate 2019
dc.date.none.fl_str_mv 2019
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/158564
url http://hdl.handle.net/10366/158564
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elservier
publisher.none.fl_str_mv Elservier
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425224970141696
score 15,198674

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