Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain

Background: Different species of apicomplexan protozoans of the genera Hepatozoon and Cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. The aim of this study was to assess the molecular prevalence of Hepatozoon spp. and Cytauxzoon spp. and t...

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Autores: Díaz-Regañón Fernández, David Rafael, Villaescusa Fernández, Alejandra, Ayllón Santiago, Tania, Rodríguez Franco, Fernando, Baneth, Gad, Calleja-Bueno, Lydia, García-Sancho Téllez, Mercedes Guadalupe, Agulla Pérez, Beatriz, Sainz Rodríguez, Ángel
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/115224
Acesso em linha:https://hdl.handle.net/20.500.14352/115224
Access Level:acceso abierto
Palavra-chave:579.62
Cat
Central Spain
Cytauxzoon sp.
Hepatozoon canis
Hepatozoon felis
PCR
Microbiología (Veterinaria)
3109 Ciencias Veterinarias
3109.05 Microbiología
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spelling Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, SpainDíaz-Regañón Fernández, David RafaelVillaescusa Fernández, AlejandraAyllón Santiago, TaniaRodríguez Franco, FernandoBaneth, GadCalleja-Bueno, LydiaGarcía-Sancho Téllez, Mercedes GuadalupeAgulla Pérez, BeatrizSainz Rodríguez, Ángel579.62CatCentral SpainCytauxzoon sp.Hepatozoon canisHepatozoon felisPCRMicrobiología (Veterinaria)3109 Ciencias Veterinarias3109.05 MicrobiologíaBackground: Different species of apicomplexan protozoans of the genera Hepatozoon and Cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. The aim of this study was to assess the molecular prevalence of Hepatozoon spp. and Cytauxzoon spp. and to identify associated risk factors and clinical and laboratory abnormalities in a population of cats from Madrid, Spain. Methods: Six hundred and forty-four client-owned and stray cats from Madrid, Spain, were included in this study. DNA samples were analyzed by two polymerase chain reaction (PCR) tests to detect a partial sequence of the 18S rRNA gene of Hepatozoon spp. and Cytauxzoon spp. In order to evaluate possible associations between infection by these protozoans and epidemiological or clinical parameters, data were collected related to: the season of sample collection, age, gender, spayed/neutered status, breed, living area, lifestyle, outdoor access, contact with other animals, prey on wild animals, history of tick or flea infestation, travel history, ectoparasiticide treatment, previous blood transfusion, previous tetracycline administration in the last 60 days, Feline Leukemia virus (FeLV) and Feline Immunodeficiency virus (FIV) status, positivity to other vector-borne diseases, the presence or absence of clinical signs and hematological or biochemical alterations. Results: DNA of Hepatozoon spp. and Cytauxzoon sp. was amplified from the blood of 10 (1.6%) and 8 (1.2%) cats, respectively. Previous treatment with tetracyclines in the last 60 days, previous administration of blood transfusion, a decrease in haematocrit and an increase in creatinine were associated with Hepatozoon spp. infection. Cytauxzoon sp. infection was more frequent in samples collected during the winter months and in cats living in rural areas. This infection was associated with a FIV-positive status. Some of the cats that were positive for Hepatozoon spp. or Cytauxzoon sp. had been exposed to other vector-borne pathogens, such as Ehrlichia canis and Bartonella henselae. Conclusions: Our results indicate that cats from Madrid, central Spain, are infected with Hepatozoon spp. and Cytauxzoon sp., although with a low prevalence. Further studies are needed to determine the virulence of these agents in Spanish cats.BioMed CentralUniversidad Complutense de Madrid20172017-03-1320172017-03-13journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/115224reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1152242026-06-02T12:44:21Z
dc.title.none.fl_str_mv Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain
title Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain
spellingShingle Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain
Díaz-Regañón Fernández, David Rafael
579.62
Cat
Central Spain
Cytauxzoon sp.
Hepatozoon canis
Hepatozoon felis
PCR
Microbiología (Veterinaria)
3109 Ciencias Veterinarias
3109.05 Microbiología
title_short Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain
title_full Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain
title_fullStr Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain
title_full_unstemmed Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain
title_sort Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain
dc.creator.none.fl_str_mv Díaz-Regañón Fernández, David Rafael
Villaescusa Fernández, Alejandra
Ayllón Santiago, Tania
Rodríguez Franco, Fernando
Baneth, Gad
Calleja-Bueno, Lydia
García-Sancho Téllez, Mercedes Guadalupe
Agulla Pérez, Beatriz
Sainz Rodríguez, Ángel
author Díaz-Regañón Fernández, David Rafael
author_facet Díaz-Regañón Fernández, David Rafael
Villaescusa Fernández, Alejandra
Ayllón Santiago, Tania
Rodríguez Franco, Fernando
Baneth, Gad
Calleja-Bueno, Lydia
García-Sancho Téllez, Mercedes Guadalupe
Agulla Pérez, Beatriz
Sainz Rodríguez, Ángel
author_role author
author2 Villaescusa Fernández, Alejandra
Ayllón Santiago, Tania
Rodríguez Franco, Fernando
Baneth, Gad
Calleja-Bueno, Lydia
García-Sancho Téllez, Mercedes Guadalupe
Agulla Pérez, Beatriz
Sainz Rodríguez, Ángel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579.62
Cat
Central Spain
Cytauxzoon sp.
Hepatozoon canis
Hepatozoon felis
PCR
Microbiología (Veterinaria)
3109 Ciencias Veterinarias
3109.05 Microbiología
topic 579.62
Cat
Central Spain
Cytauxzoon sp.
Hepatozoon canis
Hepatozoon felis
PCR
Microbiología (Veterinaria)
3109 Ciencias Veterinarias
3109.05 Microbiología
description Background: Different species of apicomplexan protozoans of the genera Hepatozoon and Cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. The aim of this study was to assess the molecular prevalence of Hepatozoon spp. and Cytauxzoon spp. and to identify associated risk factors and clinical and laboratory abnormalities in a population of cats from Madrid, Spain. Methods: Six hundred and forty-four client-owned and stray cats from Madrid, Spain, were included in this study. DNA samples were analyzed by two polymerase chain reaction (PCR) tests to detect a partial sequence of the 18S rRNA gene of Hepatozoon spp. and Cytauxzoon spp. In order to evaluate possible associations between infection by these protozoans and epidemiological or clinical parameters, data were collected related to: the season of sample collection, age, gender, spayed/neutered status, breed, living area, lifestyle, outdoor access, contact with other animals, prey on wild animals, history of tick or flea infestation, travel history, ectoparasiticide treatment, previous blood transfusion, previous tetracycline administration in the last 60 days, Feline Leukemia virus (FeLV) and Feline Immunodeficiency virus (FIV) status, positivity to other vector-borne diseases, the presence or absence of clinical signs and hematological or biochemical alterations. Results: DNA of Hepatozoon spp. and Cytauxzoon sp. was amplified from the blood of 10 (1.6%) and 8 (1.2%) cats, respectively. Previous treatment with tetracyclines in the last 60 days, previous administration of blood transfusion, a decrease in haematocrit and an increase in creatinine were associated with Hepatozoon spp. infection. Cytauxzoon sp. infection was more frequent in samples collected during the winter months and in cats living in rural areas. This infection was associated with a FIV-positive status. Some of the cats that were positive for Hepatozoon spp. or Cytauxzoon sp. had been exposed to other vector-borne pathogens, such as Ehrlichia canis and Bartonella henselae. Conclusions: Our results indicate that cats from Madrid, central Spain, are infected with Hepatozoon spp. and Cytauxzoon sp., although with a low prevalence. Further studies are needed to determine the virulence of these agents in Spanish cats.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-03-13
2017
2017-03-13
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/115224
url https://hdl.handle.net/20.500.14352/115224
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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