Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability

Hyperlipidic diets limit glucose oxidation and favor amino acid preservation, hampering the elimination of excess dietary nitrogen and the catabolic utilization of amino acids.We analyzed whether reduced urea excretion was a consequence of higherNO; (nitrite,nitrate, and other derivatives) availabil...

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Autores: Sabater Martínez, David, Agnelli, Silvia, Arriarán, Sofía, Fernández López, José Antonio, Romero Romero, María del Mar, Alemany, Marià, 1946-, Remesar Betlloch, Xavier
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/53473
Acesso em linha:https://hdl.handle.net/2445/53473
Access Level:acceso abierto
Palavra-chave:Síndrome metabòlica
Metabolisme
Dieta
Urea
Nitrogen
Aminoàcids
Rates (Animals de laboratori)
Metabolic syndrome
Metabolism
Diet
Amino acids
Rats as laboratory animals
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spelling Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availabilitySabater Martínez, DavidAgnelli, SilviaArriarán, SofíaFernández López, José AntonioRomero Romero, María del MarAlemany, Marià, 1946-Remesar Betlloch, XavierSíndrome metabòlicaMetabolismeDietaUreaNitrogenAminoàcidsRates (Animals de laboratori)Metabolic syndromeMetabolismDietAmino acidsRats as laboratory animalsHyperlipidic diets limit glucose oxidation and favor amino acid preservation, hampering the elimination of excess dietary nitrogen and the catabolic utilization of amino acids.We analyzed whether reduced urea excretion was a consequence of higherNO; (nitrite,nitrate, and other derivatives) availability caused by increased nitric oxide production in metabolic syndrome. Rats fed a cafeteria diet for 30 days had a higher intake and accumulation of amino acid nitrogen and lower urea excretion.There were no differences in plasma nitrate or nitrite. NO and creatinine excretion accounted for only a small part of total nitrogen excretion. Rats fed a cafeteria diet had higher plasma levels of glutamine, serine, threonine, glycine, and ornithinewhen comparedwith controls,whereas arginine was lower. Liver carbamoyl-phosphate synthetase I activity was higher in cafeteria diet-fed rats, but arginase I was lower. The high carbamoyl-phosphate synthetase activity and ornithine levels suggest activation of the urea cycle in cafeteria diet-fed rats, but low arginine levels point to a block in the urea cycle between ornithine and arginine, thereby preventing the elimination of excess nitrogen as urea. The ultimate consequence of this paradoxical block in the urea cycle seems to be the limitation of arginine production and/or availability.Hindawi Publishing Corporation2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/53473Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1155/2014/959420BioMed Research International, 2014, vol. 2014http://dx.doi.org/10.1155/2014/959420cc-by (c) Sabater Martínez, David et al., 2014http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/534732026-05-27T06:46:51Z
dc.title.none.fl_str_mv Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability
title Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability
spellingShingle Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability
Sabater Martínez, David
Síndrome metabòlica
Metabolisme
Dieta
Urea
Nitrogen
Aminoàcids
Rates (Animals de laboratori)
Metabolic syndrome
Metabolism
Diet
Amino acids
Rats as laboratory animals
title_short Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability
title_full Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability
title_fullStr Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability
title_full_unstemmed Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability
title_sort Altered nitrogen balance and decreased urea excretion in male rats fed cafeteria diet are related to arginine availability
dc.creator.none.fl_str_mv Sabater Martínez, David
Agnelli, Silvia
Arriarán, Sofía
Fernández López, José Antonio
Romero Romero, María del Mar
Alemany, Marià, 1946-
Remesar Betlloch, Xavier
author Sabater Martínez, David
author_facet Sabater Martínez, David
Agnelli, Silvia
Arriarán, Sofía
Fernández López, José Antonio
Romero Romero, María del Mar
Alemany, Marià, 1946-
Remesar Betlloch, Xavier
author_role author
author2 Agnelli, Silvia
Arriarán, Sofía
Fernández López, José Antonio
Romero Romero, María del Mar
Alemany, Marià, 1946-
Remesar Betlloch, Xavier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Síndrome metabòlica
Metabolisme
Dieta
Urea
Nitrogen
Aminoàcids
Rates (Animals de laboratori)
Metabolic syndrome
Metabolism
Diet
Amino acids
Rats as laboratory animals
topic Síndrome metabòlica
Metabolisme
Dieta
Urea
Nitrogen
Aminoàcids
Rates (Animals de laboratori)
Metabolic syndrome
Metabolism
Diet
Amino acids
Rats as laboratory animals
description Hyperlipidic diets limit glucose oxidation and favor amino acid preservation, hampering the elimination of excess dietary nitrogen and the catabolic utilization of amino acids.We analyzed whether reduced urea excretion was a consequence of higherNO; (nitrite,nitrate, and other derivatives) availability caused by increased nitric oxide production in metabolic syndrome. Rats fed a cafeteria diet for 30 days had a higher intake and accumulation of amino acid nitrogen and lower urea excretion.There were no differences in plasma nitrate or nitrite. NO and creatinine excretion accounted for only a small part of total nitrogen excretion. Rats fed a cafeteria diet had higher plasma levels of glutamine, serine, threonine, glycine, and ornithinewhen comparedwith controls,whereas arginine was lower. Liver carbamoyl-phosphate synthetase I activity was higher in cafeteria diet-fed rats, but arginase I was lower. The high carbamoyl-phosphate synthetase activity and ornithine levels suggest activation of the urea cycle in cafeteria diet-fed rats, but low arginine levels point to a block in the urea cycle between ornithine and arginine, thereby preventing the elimination of excess nitrogen as urea. The ultimate consequence of this paradoxical block in the urea cycle seems to be the limitation of arginine production and/or availability.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/53473
url https://hdl.handle.net/2445/53473
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1155/2014/959420
BioMed Research International, 2014, vol. 2014
http://dx.doi.org/10.1155/2014/959420
dc.rights.none.fl_str_mv cc-by (c) Sabater Martínez, David et al., 2014
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Sabater Martínez, David et al., 2014
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674