LC–MS/MS simultaneous analysis of allopregnanolone, epiallopregnanolone, pregnanolone, dehydroepiandrosterone and dehydroepiandrosterone 3-sulfate in human plasma
Aim: Several neuropsychopharmacological properties have been attributed to the 3#945;-reduced pregnane steroids, allopregnanolone and pregnanolone, as well as to dehydroepiandrosterone sulfate because of their ability to modulate #947;-aminobutyric acid (GABAA) receptors in the CNS. In order to unde...
| Authors: | , , , , , , , , , , , |
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| Format: | article |
| Publication Date: | 2017 |
| Country: | España |
| Institution: | Universidad de Castilla-La Mancha |
| Repository: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/13151 |
| Online Access: | http://hdl.handle.net/10578/13151 |
| Access Level: | Open access |
| Keyword: | Allopregnanolone Dehydroepiandrosterone Mass spectrometry Neurosteroids Plasma Pregnanolone |
| Summary: | Aim: Several neuropsychopharmacological properties have been attributed to the 3#945;-reduced pregnane steroids, allopregnanolone and pregnanolone, as well as to dehydroepiandrosterone sulfate because of their ability to modulate #947;-aminobutyric acid (GABAA) receptors in the CNS. In order to understand better their role in several mechanisms in CNS, a new methodology is proposed to monitor these compounds in human plasma. Methodology results: The analytes were first derivatized with 2-hydrazinopyridine and extracted from plasma using SPE. Then, the compounds were separated and detected by LC?MS/MS. A mobile phase of formic acid (0.1%) in water and methanol through a gradient of composition and a flow rate of 0.3 ml min-1 resulted in good separations of the analytes. Linear responses in wide range of concentrations and LOQs ranging from 10 (dehydroepiandrosterone 3-sulfate) to 40 pg ml-1 (dehydroepiandrosterone) were obtained in |
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