Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis

12 pages, 8 figures.-- PMID: 18256290 [PubMed].-- PMCID: PMC2291433.-- Printed version published Apr 2008.

Detalles Bibliográficos
Autores: Pinar, Mario, Coll, Pedro M., Rincón, Sergio A., Pérez González, Pilar
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/11172
Acceso en línea:http://hdl.handle.net/10261/11172
Access Level:acceso abierto
Palabra clave:Schizosaccharomyces pombe
Cytokinesis
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spelling Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesisPinar, MarioColl, Pedro M.Rincón, Sergio A.Pérez González, PilarSchizosaccharomyces pombeCytokinesis12 pages, 8 figures.-- PMID: 18256290 [PubMed].-- PMCID: PMC2291433.-- Printed version published Apr 2008.Supporting information (Suppl. Figures S1-S6 and Videos S1-S6) available at: http://www.molbiolcell.org/cgi/content/full/E07-07-0718/DC1Schizosaccharomyces pombe Rho GTPases regulate actin cytoskeleton organization and cell integrity. We studied the fission yeast gene SPBC4F6.12 based on its ability to suppress the thermosensitivity of cdc42-1625 mutant strain. This gene, named pxl1 , encodes a protein with three LIM domains that is similar to paxillin. Pxl1 does not interact with Cdc42 but it interacts with Rho1, and it negatively regulates this GTPase. Fission yeast Pxl1 forms a contractile ring in the cell division region and deletion of pxl1 causes a delay in cell– cell separation, suggesting that it has a function in cytokinesis. Pxl1 N-terminal region is required and sufficient for its localization to the medial ring, whereas the LIM domains are necessary for its function. Pxl1 localization requires actin polymerization and the actomyosin ring, but it is independent of the septation initiation network (SIN) function. Moreover, Pxl1 colocalizes and interacts with Myo2, and Cdc15, suggesting that it is part of the actomyosin ring. Here, we show that in cells lacking Pxl1, the myosin ring is not correctly assembled and that actomyosin ring contraction is delayed. Together, these data suggest that Pxl1 modulates Rho1 GTPase signaling and plays a role in the formation and contraction of the actomyosin ring during cytokinesis.M. Pinar was supported by a fellowship from the Spanish Ministerio de Educación. This work was supported by grant BIO2004-0834 from the Comisión Interministerial de Ciencia y Tecnología, Spain.Peer reviewedAmerican Society for Cell Biology200920092008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65012917252 bytesapplication/pdfhttp://hdl.handle.net/10261/11172reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1091/mbc.E07-07-0718info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/111722026-05-22T06:33:51Z
dc.title.none.fl_str_mv Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis
title Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis
spellingShingle Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis
Pinar, Mario
Schizosaccharomyces pombe
Cytokinesis
title_short Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis
title_full Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis
title_fullStr Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis
title_full_unstemmed Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis
title_sort Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis
dc.creator.none.fl_str_mv Pinar, Mario
Coll, Pedro M.
Rincón, Sergio A.
Pérez González, Pilar
author Pinar, Mario
author_facet Pinar, Mario
Coll, Pedro M.
Rincón, Sergio A.
Pérez González, Pilar
author_role author
author2 Coll, Pedro M.
Rincón, Sergio A.
Pérez González, Pilar
author2_role author
author
author
dc.subject.none.fl_str_mv Schizosaccharomyces pombe
Cytokinesis
topic Schizosaccharomyces pombe
Cytokinesis
description 12 pages, 8 figures.-- PMID: 18256290 [PubMed].-- PMCID: PMC2291433.-- Printed version published Apr 2008.
publishDate 2008
dc.date.none.fl_str_mv 2008
2009
2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/11172
url http://hdl.handle.net/10261/11172
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1091/mbc.E07-07-0718
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2917252 bytes
application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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