Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon

Gastrointestinal motility disturbances are a hallmark of inflammatory bowel disease (IBD); however, their mechanisms remain unclear. This study used a dextran sulfate sodium–induced colitis mouse model, deficient in mature B and T lymphocytes, to assess intestinal motility and the role of the adapti...

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Detalhes bibliográficos
Autores: Gomez-Bris, Raquel, Rodríguez-Rodríguez, Pilar, Ortega-Zapero, Marina, Ruvira, Santiago, Castillo-González, Raquel, Fernández-Aceñero, María Jesús, Cruz-Adalia, Aránzazu, Saez, Angela, Arribas, Silvia Magdalena, Gonzalez-Granado, Jose Maria
Formato: artículo
Fecha de publicación:2025
País:España
Recursos:Universidad Francisco de Vitoria
Repositorio:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
Idioma:inglés
OAI Identifier:oai:ddfv.ufv.es:10641/7143
Acesso em linha:https://hdl.handle.net/10641/7143
Access Level:acceso abierto
Palavra-chave:Pathology and Forensic Medicine
Journal Article
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yes
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spelling Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and ColonGomez-Bris, RaquelRodríguez-Rodríguez, PilarOrtega-Zapero, MarinaRuvira, SantiagoCastillo-González, RaquelFernández-Aceñero, María JesúsCruz-Adalia, AránzazuSaez, AngelaArribas, Silvia MagdalenaGonzalez-Granado, Jose MariaPathology and Forensic MedicineJournal ArticleYesyesGastrointestinal motility disturbances are a hallmark of inflammatory bowel disease (IBD); however, their mechanisms remain unclear. This study used a dextran sulfate sodium–induced colitis mouse model, deficient in mature B and T lymphocytes, to assess intestinal motility and the role of the adaptive immune system in health and IBD. In healthy mice, the absence of adaptive lymphocytes reduced acetylcholine (ACh) sensitivity in the ileum. During colitis, it decreases motility by reducing the intensity and frequency of spontaneous contractions while increasing cholinergic responsiveness. In the proximal colon, adaptive immunity deficiency led to increased contractility and reduced ACh sensitivity in homeostasis, whereas colitis reduced contractile capacity. In the mid colon, immune-deficient mice had reduced ACh sensitivity in homeostasis and exacerbated contractile responses during colitis. In the distal colon, adaptive immunity loss reduced contractility in health and cholinergic responsiveness during colitis. These motility alterations were associated with altered acetylcholinesterase and M2/M3 muscarinic receptor expression. Notably, adaptive lymphocyte deficiency resulted in reduced tissue damage and lower tumor necrosis factor-α expression in the colon during colitis, paralleling intestinal motility changes. Overall, the adaptive immune system critically regulates motility and inflammation across different intestinal segments in IBD.Facultad de Ciencias Experimentales20252025-02-0120252025-02-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10641/7143reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoriainstname:Universidad Francisco de VitoriaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddfv.ufv.es:10641/71432026-06-11T12:44:57Z
dc.title.none.fl_str_mv Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon
title Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon
spellingShingle Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon
Gomez-Bris, Raquel
Pathology and Forensic Medicine
Journal Article
Yes
yes
title_short Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon
title_full Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon
title_fullStr Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon
title_full_unstemmed Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon
title_sort Segmental Regulation of Intestinal Motility by Colitis and the Adaptive Immune System in the Mouse Ileum and Colon
dc.creator.none.fl_str_mv Gomez-Bris, Raquel
Rodríguez-Rodríguez, Pilar
Ortega-Zapero, Marina
Ruvira, Santiago
Castillo-González, Raquel
Fernández-Aceñero, María Jesús
Cruz-Adalia, Aránzazu
Saez, Angela
Arribas, Silvia Magdalena
Gonzalez-Granado, Jose Maria
author Gomez-Bris, Raquel
author_facet Gomez-Bris, Raquel
Rodríguez-Rodríguez, Pilar
Ortega-Zapero, Marina
Ruvira, Santiago
Castillo-González, Raquel
Fernández-Aceñero, María Jesús
Cruz-Adalia, Aránzazu
Saez, Angela
Arribas, Silvia Magdalena
Gonzalez-Granado, Jose Maria
author_role author
author2 Rodríguez-Rodríguez, Pilar
Ortega-Zapero, Marina
Ruvira, Santiago
Castillo-González, Raquel
Fernández-Aceñero, María Jesús
Cruz-Adalia, Aránzazu
Saez, Angela
Arribas, Silvia Magdalena
Gonzalez-Granado, Jose Maria
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Facultad de Ciencias Experimentales
dc.subject.none.fl_str_mv Pathology and Forensic Medicine
Journal Article
Yes
yes
topic Pathology and Forensic Medicine
Journal Article
Yes
yes
description Gastrointestinal motility disturbances are a hallmark of inflammatory bowel disease (IBD); however, their mechanisms remain unclear. This study used a dextran sulfate sodium–induced colitis mouse model, deficient in mature B and T lymphocytes, to assess intestinal motility and the role of the adaptive immune system in health and IBD. In healthy mice, the absence of adaptive lymphocytes reduced acetylcholine (ACh) sensitivity in the ileum. During colitis, it decreases motility by reducing the intensity and frequency of spontaneous contractions while increasing cholinergic responsiveness. In the proximal colon, adaptive immunity deficiency led to increased contractility and reduced ACh sensitivity in homeostasis, whereas colitis reduced contractile capacity. In the mid colon, immune-deficient mice had reduced ACh sensitivity in homeostasis and exacerbated contractile responses during colitis. In the distal colon, adaptive immunity loss reduced contractility in health and cholinergic responsiveness during colitis. These motility alterations were associated with altered acetylcholinesterase and M2/M3 muscarinic receptor expression. Notably, adaptive lymphocyte deficiency resulted in reduced tissue damage and lower tumor necrosis factor-α expression in the colon during colitis, paralleling intestinal motility changes. Overall, the adaptive immune system critically regulates motility and inflammation across different intestinal segments in IBD.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-02-01
2025
2025-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10641/7143
url https://hdl.handle.net/10641/7143
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
instname:Universidad Francisco de Vitoria
instname_str Universidad Francisco de Vitoria
reponame_str DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
collection DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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