Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring

This contains three Microsoft Excel files for global methylation, AFLP and MSAP data, respectively. On each file one sheet contains the information of maternal plants and the other the information of the offspring. Each row refers to individual samples. For AFLP and MSAP each column refers to specif...

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Detalhes bibliográficos
Autores: Herrera, Carlos M., Alonso, Conchita, Medrano, Mónica, Pérez, Ricardo, Bazaga, Pilar
Formato: conjunto de datos
Fecha de publicación:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/157179
Acesso em linha:http://hdl.handle.net/10261/157179
Access Level:acceso abierto
Palavra-chave:Amplified fragment length polymorphisms (AFLP)
Epigenetic variation
Global DNA methylation
HPLC
Inheritance
Lavandula latifolia
Methylation-sensitive amplified fragment length polymorphisms (MSAP)
Spain
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spelling Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspringHerrera, Carlos M.Alonso, ConchitaMedrano, MónicaPérez, RicardoBazaga, PilarAmplified fragment length polymorphisms (AFLP)Epigenetic variationGlobal DNA methylationHPLCInheritanceLavandula latifoliaMethylation-sensitive amplified fragment length polymorphisms (MSAP)SpainThis contains three Microsoft Excel files for global methylation, AFLP and MSAP data, respectively. On each file one sheet contains the information of maternal plants and the other the information of the offspring. Each row refers to individual samples. For AFLP and MSAP each column refers to specific (although anonymous) markers labeled similarly in both sheets. Leaves from 15 adult plants were sampled along a 125-m transect in Cuevas Bermejas population (Sierra de Cazorla, Jaén Province, southeastern Spain), in October 2015. Offspring were obtained from seeds under greenhouse conditions, leaf samples were collected when they were 8-months old when only offspring of 13 mothers remained. DNA was extracted from leaves using Qiagen DNeasy Plant Mini Kit and the manufacturer protocol. Maternal parents and greenhouse plants were genetically fingerprinted using amplified fragment polymorphism (AFLP) markers. The AFLP and MSAP analysis were performed using standard protocols involving the use of fluorescent dye-labeled selective primers. For AFLP, each plant was fingerprinted using three different EcoRI + 3 / MseI + 3 primer pair combinations. MSAP assays used four HpaII-MspI + 2 / MseI + 3 primer combinations. In the two analyses fragment separation and detection was made using an ABI PRISM 3130xl DNA sequencer, only fragments ≥ 150 base pairs in size were considered and the presence (1) or absence (0) of fragments in each sample was scored manually by visualizing electropherograms with GeneMapper 3.7 softwareThis dataset includes (i) global DNA cytosine methylation estimates (= percentage of all genomic cytosines that are methylated) obtained by reverse phase HPLC with spectrofluorimetric detection, (ii) the scoring (presence/absence) of individual genetic markers (AFLP), and (iii) the scoring (presence/absence) of individual epigenetic markers (MSAP) in maternal plants of the shrub Lavandula latifolia and their greenhouse-grown offspring.Peer reviewedHerrera, Carlos M. <herrera@ebd.csic.es>Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1http://hdl.handle.net/10261/157179reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésHerrera, Carlos M.; Alonso, Conchita; Medrano, Mónica; Pérez, Ricardo; Bazaga, Pilar. Transgenerational epigenetics: inheritance of global cytosine methylation and methylation-related epigenetic markers in the shrub. Lavandula latifolia. American Journal of Botany. http://dx.doi.org/10.1002/ajb2.1074Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1571792026-05-22T06:33:51Z
dc.title.none.fl_str_mv Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring
title Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring
spellingShingle Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring
Herrera, Carlos M.
Amplified fragment length polymorphisms (AFLP)
Epigenetic variation
Global DNA methylation
HPLC
Inheritance
Lavandula latifolia
Methylation-sensitive amplified fragment length polymorphisms (MSAP)
Spain
title_short Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring
title_full Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring
title_fullStr Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring
title_full_unstemmed Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring
title_sort Global cytosine methylation, genetic markers (AFLP) and epigenetic markers (MSAP) in 15 maternal plants of the shrub Lavandula latifolia (Lamiaceae) and their offspring
dc.creator.none.fl_str_mv Herrera, Carlos M.
Alonso, Conchita
Medrano, Mónica
Pérez, Ricardo
Bazaga, Pilar
author Herrera, Carlos M.
author_facet Herrera, Carlos M.
Alonso, Conchita
Medrano, Mónica
Pérez, Ricardo
Bazaga, Pilar
author_role author
author2 Alonso, Conchita
Medrano, Mónica
Pérez, Ricardo
Bazaga, Pilar
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Herrera, Carlos M. <herrera@ebd.csic.es>
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Amplified fragment length polymorphisms (AFLP)
Epigenetic variation
Global DNA methylation
HPLC
Inheritance
Lavandula latifolia
Methylation-sensitive amplified fragment length polymorphisms (MSAP)
Spain
topic Amplified fragment length polymorphisms (AFLP)
Epigenetic variation
Global DNA methylation
HPLC
Inheritance
Lavandula latifolia
Methylation-sensitive amplified fragment length polymorphisms (MSAP)
Spain
description This contains three Microsoft Excel files for global methylation, AFLP and MSAP data, respectively. On each file one sheet contains the information of maternal plants and the other the information of the offspring. Each row refers to individual samples. For AFLP and MSAP each column refers to specific (although anonymous) markers labeled similarly in both sheets. Leaves from 15 adult plants were sampled along a 125-m transect in Cuevas Bermejas population (Sierra de Cazorla, Jaén Province, southeastern Spain), in October 2015. Offspring were obtained from seeds under greenhouse conditions, leaf samples were collected when they were 8-months old when only offspring of 13 mothers remained. DNA was extracted from leaves using Qiagen DNeasy Plant Mini Kit and the manufacturer protocol. Maternal parents and greenhouse plants were genetically fingerprinted using amplified fragment polymorphism (AFLP) markers. The AFLP and MSAP analysis were performed using standard protocols involving the use of fluorescent dye-labeled selective primers. For AFLP, each plant was fingerprinted using three different EcoRI + 3 / MseI + 3 primer pair combinations. MSAP assays used four HpaII-MspI + 2 / MseI + 3 primer combinations. In the two analyses fragment separation and detection was made using an ABI PRISM 3130xl DNA sequencer, only fragments ≥ 150 base pairs in size were considered and the presence (1) or absence (0) of fragments in each sample was scored manually by visualizing electropherograms with GeneMapper 3.7 software
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/157179
url http://hdl.handle.net/10261/157179
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Herrera, Carlos M.; Alonso, Conchita; Medrano, Mónica; Pérez, Ricardo; Bazaga, Pilar. Transgenerational epigenetics: inheritance of global cytosine methylation and methylation-related epigenetic markers in the shrub. Lavandula latifolia. American Journal of Botany. http://dx.doi.org/10.1002/ajb2.1074
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424174860075008
score 15,228081

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