Modulation of equol production via different dietary regimens in an artificial model of the human colon
In order to find dietary conditions favouring endogenous equol biosynthesis, a pooled faecal homogenate from equol-producing women was used to inoculate the TIM-2 artificial model of the human proximal colon. The model was fuelled with control diets not supplemented (C) or supplemented (C-ISO) with...
| Authors: | , , , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2020 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/223094 |
| Online Access: | http://hdl.handle.net/10261/223094 |
| Access Level: | Open access |
| Keyword: | Soy isoflavones Daidzein Equol Intestinal model TIM-2 Human faeces Intestinal microbiota |
| Summary: | In order to find dietary conditions favouring endogenous equol biosynthesis, a pooled faecal homogenate from equol-producing women was used to inoculate the TIM-2 artificial model of the human proximal colon. The model was fuelled with control diets not supplemented (C) or supplemented (C-ISO) with isoflavones, and two isoflavone-containing diets rich in carbohydrate (CH-ISO) or protein (PR-ISO). Compared to the C-ISO control, the CH-ISO diet doubled the production of equol, while with the PR-ISO diet the production of equol in cultures decreased sharply. The CH-ISO diet was also associated with enhanced butyrate production. The numbers of most bacterial populations analysed did not significantly change along cultures with any of the diets. Surprisingly, counts for a gene involved in equol production (tdr) were reduced in all cultures, reflecting a reduction in the number of equol-producing bacteria. In conclusion, under the TIM-2 culture conditions established, the CH-ISO diet favoured the synthesis of equol. |
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