Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock

Grapevine trunk diseases (GTDs) are caused by cryptic complexes of fungal pathogens andhave become a growing problem for new grapevine (Vitis vinifera) plantations. We studied the roleof the nursery, variety, and rootstock in the composition of the fungal communities in root collarsand graft unions...

Full description

Bibliographic Details
Authors: Lade Lee, Sarah Boyd, Oliva Palau, Jonàs, Štraus, Dora
Format: article
Status:Published version
Publication Date:2022
Country:España
Institution:Universitat de Lleida (UdL)
Repository:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/73259
Online Access:https://doi.org/10.3390/jof8010047
http://hdl.handle.net/10459.1/73259
Access Level:Open access
Keyword:Grapevine trunk diseases (GTDs)
Vitis vinifera
Metabarcoding
id ES_eea626b73f9fe8f74817b221cd3d374d
oai_identifier_str oai:repositori.udl.cat:10459.1/73259
network_acronym_str ES
network_name_str España
repository_id_str
spelling Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstockLade Lee, Sarah BoydOliva Palau, JonàsŠtraus, DoraGrapevine trunk diseases (GTDs)Vitis viniferaMetabarcodingGrapevine trunk diseases (GTDs) are caused by cryptic complexes of fungal pathogens andhave become a growing problem for new grapevine (Vitis vinifera) plantations. We studied the roleof the nursery, variety, and rootstock in the composition of the fungal communities in root collarsand graft unions of planting material in Catalonia (NE Spain). We compared necrosis and fungalcommunities in graft unions and root collars at harvest, and then after three months of cold storage.We evaluated combinations of eleven red and five white varieties with four common rootstockscoming from six nurseries. Fungal communities were characterized by isolation and metabarcodingof the ITS2 region. Our data suggests that nursery followed by rootstock and variety had significanteffects on necrosis and fungal community structure in graft and root tissues. Within the plant, wefound large differences in terms fungal community distribution between graft and root tissues. Graftunions housed a significantly higher relative abundance of GTD-related Operational Taxonomic Units(OTUs) than root collars. More severe necrosis was correlated with a lower relative abundance ofGTD-related OTUs based on isolation and metabarcoding analyses. Our results suggest that nurseriesand therefore their plant production practices play a major role in determining the fungal and GTD-related fungal community in grapevine plants sold for planting. GTD variation across rootstocks andvarieties could be explored as a venue for minimizing pathogen load in young plantations.MPDI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/jof8010047http://hdl.handle.net/10459.1/73259reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a https://doi.org/10.3390/jof8010047Journal of fungi, 2022, vol. 8, núm.1, p. 1-21cc-by (c) Lade et al., 2022info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/732592026-06-24T12:42:17Z
dc.title.none.fl_str_mv Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock
title Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock
spellingShingle Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock
Lade Lee, Sarah Boyd
Grapevine trunk diseases (GTDs)
Vitis vinifera
Metabarcoding
title_short Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock
title_full Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock
title_fullStr Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock
title_full_unstemmed Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock
title_sort Variation in fungal community in grapevine (vitis vinifera) nursery stock depends on nursery, variety and rootstock
dc.creator.none.fl_str_mv Lade Lee, Sarah Boyd
Oliva Palau, Jonàs
Štraus, Dora
author Lade Lee, Sarah Boyd
author_facet Lade Lee, Sarah Boyd
Oliva Palau, Jonàs
Štraus, Dora
author_role author
author2 Oliva Palau, Jonàs
Štraus, Dora
author2_role author
author
dc.subject.none.fl_str_mv Grapevine trunk diseases (GTDs)
Vitis vinifera
Metabarcoding
topic Grapevine trunk diseases (GTDs)
Vitis vinifera
Metabarcoding
description Grapevine trunk diseases (GTDs) are caused by cryptic complexes of fungal pathogens andhave become a growing problem for new grapevine (Vitis vinifera) plantations. We studied the roleof the nursery, variety, and rootstock in the composition of the fungal communities in root collarsand graft unions of planting material in Catalonia (NE Spain). We compared necrosis and fungalcommunities in graft unions and root collars at harvest, and then after three months of cold storage.We evaluated combinations of eleven red and five white varieties with four common rootstockscoming from six nurseries. Fungal communities were characterized by isolation and metabarcodingof the ITS2 region. Our data suggests that nursery followed by rootstock and variety had significanteffects on necrosis and fungal community structure in graft and root tissues. Within the plant, wefound large differences in terms fungal community distribution between graft and root tissues. Graftunions housed a significantly higher relative abundance of GTD-related Operational Taxonomic Units(OTUs) than root collars. More severe necrosis was correlated with a lower relative abundance ofGTD-related OTUs based on isolation and metabarcoding analyses. Our results suggest that nurseriesand therefore their plant production practices play a major role in determining the fungal and GTD-related fungal community in grapevine plants sold for planting. GTD variation across rootstocks andvarieties could be explored as a venue for minimizing pathogen load in young plantations.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/jof8010047
http://hdl.handle.net/10459.1/73259
url https://doi.org/10.3390/jof8010047
http://hdl.handle.net/10459.1/73259
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.3390/jof8010047
Journal of fungi, 2022, vol. 8, núm.1, p. 1-21
dc.rights.none.fl_str_mv cc-by (c) Lade et al., 2022
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Lade et al., 2022
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MPDI
publisher.none.fl_str_mv MPDI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869423740663627776
score 15,812455

Similar Items