Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities

11 pages, 3 figures, 3 tables. PMID:21544177[PubMed] PMCID: PMC3086187

Bibliographic Details
Authors: Antúnez, José, Fernández, Julián, Lomonte, Bruno, Angulo, Yamileth, Sanz, Libia, Pérez, Alicia, Calvete, Juan J., Gutiérrez, José María
Format: article
Publication Date:2010
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/36773
Online Access:http://hdl.handle.net/10261/36773
Access Level:Open access
Keyword:Atropoides picadoi
Atropoides mexicanus
Antivenomics
Antivenoms
Neutralization
Toxicity
Immunodepletion
Snake venom
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spelling Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activitiesAntúnez, JoséFernández, JuliánLomonte, BrunoAngulo, YamilethSanz, LibiaPérez, AliciaCalvete, Juan J.Gutiérrez, José MaríaAtropoides picadoiAtropoides mexicanusAntivenomicsAntivenomsNeutralizationToxicityImmunodepletionSnake venom11 pages, 3 figures, 3 tables. PMID:21544177[PubMed] PMCID: PMC3086187Viperid snakes of the genus Atropoides are distributed in Mexico and Central America and, owing to their size and venom yield, are capable of provoking severe envenomings in humans. This study evaluated, using an 'antivenomics' approach, the ability of a polyspecific (polyvalent) antivenom manufactured in Costa Rica to recognize the proteins of Atropoides mexicanus and A. picadoi venoms, which are not included in the immunization mixture. In addition, the neutralization of lethal, hemorrhagic, myotoxic, coagulant, proteinase and phospholipase A(2) (PLA(2)) activities of these venoms by the antivenom was assessed. The antivenom was highly-effective in immunodepleting many venom components, particularly high molecular mass P-III metalloproteinases (SVMPs), L-amino acid oxidases, and some serine proteinases and P-I SVMPs. In contrast, PLA(2)s, certain serine proteinases and P-I SVMPs, and a C type lectin-like protein were only partially immunodepleted, and two PLA(2) molecules were not depleted at all. The antivenom was able to neutralize all toxic and enzymatic activities tested, although neutralization of lethality by A. nummifer venom was achieved when a challenge dose of 3 LD(50)s of venom was used, but was iffective when 4 LD(50)s were used. These results, and previously obtained evidence on the immunoreactivity of this antivenom towards homologous and heterologous venoms, revealed the low immunogenicity of a number of venom components (PLA(2)s, CRISPs, P-I SVMPs, and some serine proteinases), underscoring the need to search for innovative immunization protocols to improve the immune response to these antigens.This study was supported by projects from the Vicerrectoría de Investigación, Universidad de Costa Rica (741-A7- 611), CRUSA-CSIC (2007CR0004), CONARE, and grants BFU2007-61563 and BFU2010-17373 from the Ministerio de Ciencia e Innovación, Madrid, SpainPeer reviewedLibrary Publishing Media201120112010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/36773reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086187/pdf/JVR-01-008.pdfinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/367732026-05-22T06:33:51Z
dc.title.none.fl_str_mv Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
title Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
spellingShingle Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
Antúnez, José
Atropoides picadoi
Atropoides mexicanus
Antivenomics
Antivenoms
Neutralization
Toxicity
Immunodepletion
Snake venom
title_short Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
title_full Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
title_fullStr Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
title_full_unstemmed Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
title_sort Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
dc.creator.none.fl_str_mv Antúnez, José
Fernández, Julián
Lomonte, Bruno
Angulo, Yamileth
Sanz, Libia
Pérez, Alicia
Calvete, Juan J.
Gutiérrez, José María
author Antúnez, José
author_facet Antúnez, José
Fernández, Julián
Lomonte, Bruno
Angulo, Yamileth
Sanz, Libia
Pérez, Alicia
Calvete, Juan J.
Gutiérrez, José María
author_role author
author2 Fernández, Julián
Lomonte, Bruno
Angulo, Yamileth
Sanz, Libia
Pérez, Alicia
Calvete, Juan J.
Gutiérrez, José María
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Atropoides picadoi
Atropoides mexicanus
Antivenomics
Antivenoms
Neutralization
Toxicity
Immunodepletion
Snake venom
topic Atropoides picadoi
Atropoides mexicanus
Antivenomics
Antivenoms
Neutralization
Toxicity
Immunodepletion
Snake venom
description 11 pages, 3 figures, 3 tables. PMID:21544177[PubMed] PMCID: PMC3086187
publishDate 2010
dc.date.none.fl_str_mv 2010
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/36773
url http://hdl.handle.net/10261/36773
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086187/pdf/JVR-01-008.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Library Publishing Media
publisher.none.fl_str_mv Library Publishing Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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