Brassinolides and IAA induce the transcription of four α-expansin genes related to development in Cicer arietinum
Four different cDNAs encoding a-expansins have been identified in Cicer arietinum (Ca-EXPA1, Ca-EXPA2, Ca-EXPA3 and Ca-EXPA4). The shared amino acid sequence similarity among the four a-expansin proteins ranged from 67 to 89%. All of them display common characteristics such as molecular mass (around...
| Autores: | , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2004 |
| País: | España |
| Recursos: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/156958 |
| Acesso em linha: | http://hdl.handle.net/10366/156958 |
| Access Level: | acceso abierto |
| Palavra-chave: | Brassinolides Cell wall Cicer arietinum Epicotyl Expansin Growth |
| Resumo: | Four different cDNAs encoding a-expansins have been identified in Cicer arietinum (Ca-EXPA1, Ca-EXPA2, Ca-EXPA3 and Ca-EXPA4). The shared amino acid sequence similarity among the four a-expansin proteins ranged from 67 to 89%. All of them display common characteristics such as molecular mass (around 24 kDa), amino acid numbers, and also the presence of a signal peptide. The transcription pattern of chickpea a-expansin genes in seedlings and plants suggests a specific role for each of the four a-expansins in different phases of development or in different plant organs. High levels of Ca-EXPA2 transcripts coincide with maximum epicotyl and stem growth, indicating an important involvement of this particular a-expansin in elongating tissues. Ca-EXPA3 would be related to radicle development, while Ca-EXPA4 seems to be involved in pod development.A considerable increase in the level of all Ca-EXPA transcripts accompanied the indole acetic acid (IAA) plus brassinolide (BR)-induced elongation of excised epicotyl segments. This IAA + BR induction was seen even for the chickpea expansin genes whose transcription was not affected by IAA or BR alone. |
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